Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter May 6, 2022

Comparison of thyroid stimulating hormone, free thyroxine, total triiodothyronine, thyroglobulin and peroxidase antibodies measurements by two different platforms

Anis Al Achkar, Elie Naous ORCID logo, Christy Salameh ORCID logo, Colin Charbel Cordahi, Myrna Germanos-Haddad, Ghassan Sleilaty and Marie-Hélène Gannagé-Yared ORCID logo

Corresponding author: Marie-Hélène Gannagé-Yared, MD MSc, Department of Endocrinology, Faculty of Medicine, Saint-Joseph University, Beirut, Lebanon, and Laboratory of Hormonology, Hôtel-Dieu de France Hospital, Department of laboratory medicine, Faculty of Medicine, Saint-Joseph University, Beirut, Lebanon, Phone: 9613291301, Fax: 9611615295, E-mail:
Anis Al Achkar and Elie Naous share first authorship.

Funding source: Conseil de recherche de l’Université Saint-Joseph, Beirut

Award Identifier / Grant number: FM406

  1. Research funding: This work was supported by a grant of the “Conseil de recherche de l’Université Saint-Joseph” FM406, Beirut.

  2. Author Contributions: AA, CS and CCC has performed the recruitment of volunteers and has contributed to the redaction of the manuscript, GS perform the statistical analysis. EN contributed to the redaction of the manuscript. MGH reviewed the manuscript and MHGY has conceived and designed the study, and wrote the manuscript. All the authors approved the final version of the paper. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The study had the approval of the ethics committee of Hôtel-Dieu de France hospital (CEHDF1524).

  6. Data availability: The data that support the findings of this study are available upon request from the corresponding author.

References

1. Beckett, G, MacKenzie, F. Thyroid guidelines - are thyroid-stimulating hormone assays fit for purpose? Ann Clin Biochem 2007;44:203–8. https://doi.org/10.1258/000456307780480945.Search in Google Scholar PubMed

2. Thienpont, LM, Van Uytfanghe, K, De Grande, LAC, Reynders, D, Das, B, Faix, JD, et al.. IFCC committee for standardization of thyroid function tests (C-STFT). Harmonization of serum thyroid-stimulating hormone measurements paves the way for the adoption of a more uniform reference interval. Clin Chem 2017;63:1248–60. https://doi.org/10.1373/clinchem.2016.269456.Search in Google Scholar PubMed

3. De Grande, LAC, Van Uytfanghe, K, Reynders, D, Das, B, Faix, JD, MacKenzie, F, et al.. IFCC committee for standardization of thyroid function tests (C-STFT). Standardization of free thyroxine measurements allows the adoption of a more uniform reference interval. Clin Chem 2017;63:1642–52. https://doi.org/10.1373/clinchem.2017.274407.Search in Google Scholar PubMed

4. Barth, JH, Luvai, A, Jassam, N, Mbagaya, W, Kilpatrick, ES, Narayanan, D, et al.. Comparison of method-related reference intervals for thyroid hormones: studies from a prospective reference population and a literature review. Ann Clin Biochem 2018;55:107–12. https://doi.org/10.1177/0004563217691549.Search in Google Scholar PubMed

5. Kratzsch, J, Baumann, NA, Ceriotti, F, Lu, ZX, Schott, M, van Herwaarden, AE, et al.. Global FT4 immunoassay standardization: an expert opinion review. Clin Chem Lab Med 2020;59:1013–23. https://doi.org/10.1515/cclm-2020-1696.Search in Google Scholar PubMed

6. La’ulu, SL, Slev, PR, Roberts, WL. Performance characteristics of 5 automated thyroglobulin autoantibody and thyroid peroxidase autoantibody assays. Clin Chim Acta 2007;376:88–95.10.1016/j.cca.2006.07.018Search in Google Scholar PubMed

7. Rahmani, N, Sebbar, E, Rifaii, S, Benhamza, N. Comparison between two automated immunoassay methods for TSH: Architect ci8200 ® versus Cobas e411®. Journal of Critical Care Obstetrics and Gynecology 2021;7:2–65.Search in Google Scholar

8. Kuzmanovska, S, Miladinova, D. Comparison of thyroid -stimulating hormone and free thyroxine immunoassays performed on immulite 2000 and Maglumi 800 automated analyzers. J Med Sci 2020;8:170–6. https://doi.org/10.3889/oamjms.2020.4318.Search in Google Scholar

9. Kazerouni, F, Amirrasouli, H. Performance characteristics of three automated immunoassays for thyroid hormones. Caspian J Intern Med 2012;3:400–104.Search in Google Scholar

10. Jonklaas, J, Sathasivam, A, Wang, H, Gu, J, Burman, KD, Soldin, SJ. Total and free thyroxine and triiodothyronine: measurement discrepancies, particularly in inpatients. Clin Biochem 2014;47:1272–8. https://doi.org/10.1016/j.clinbiochem.2014.06.007.Search in Google Scholar PubMed PubMed Central

11. Pickett, AJ, Jones, M, Evans, C. Causes of discordance between thyroglobulin antibody assays. Ann Clin Biochem 2012;49:463–7. https://doi.org/10.1258/acb.2012.012008.Search in Google Scholar PubMed

12. D’Aurizio, F, Metus, P, Ferrari, A, Caruso, B, Castello, R, Villalta, D, et al.. Definition of the upper reference limit for thyroglobulin antibodies according to the National Academy of Clinical Biochemistry guidelines: comparison of eleven different automated methods. Auto Immun Highlights 2017;8:8. https://doi.org/10.1007/s13317-017-0096-3.Search in Google Scholar PubMed PubMed Central


Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2022-0139).


Received: 2022-01-19
Revised: 2022-04-23
Accepted: 2022-04-24
Published Online: 2022-05-06
Published in Print: 2022-07-26

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Scroll Up Arrow