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Licensed Unlicensed Requires Authentication Published by De Gruyter May 5, 2022

Evaluation of serum neurofilament light in the early management of mTBI patients

  • Samy Kahouadji , Jean-Baptiste Bouillon-Minois , Charlotte Oris , Julie Durif , Bruno Pereira , Jérémy Pinguet , Agathe Rozand , Jeannot Schmidt , Vincent Sapin and Damien Bouvier EMAIL logo



Serum S100B allows a one-third reduction of computed tomography (CT) scans performed for mild traumatic brain injury (mTBI) patients. In this study, we evaluated the diagnostic performance of serum NF-L in the detection of intracranial lesions induced by mTBI.


One hundred seventy-nine adult mTBI patients presenting to the emergency department of Clermont-Ferrand University Hospital with a Glasgow Coma Scale (GCS) score of 14–15 were included. S100B assays were performed for clinical routine while NF-L samples were stored at −80 °C until analysis. CT scans were performed for patients with S100B levels above the decision threshold of 0.10 μg/L. Later, NF-L and S100B levels were compared to CT scan findings to evaluate the biomarkers’ performances.


The area under the ROC curve (AUC) evaluating the diagnostic ability in the prediction of intracranial lesions was 0.72 (95% CI; 0.58–0.87) for S100B and 0.58 (95% CI; 0.45–0.71) for NF-L, the specificities (at a threshold allowing a 100% sensitivity) were 35.7% for S100B, and 28% for NF-L (p=0.096). AUCs of NF-L and S100B for the identification of patients with neurological disorders were statistically different (p<0.001). The AUCs were 0.87 (95% CI; 0.82–0.93) for NF-L and 0.57 (95% CI; 0.48–0.66) for S100B. There was a poor correlation between NF-L and S100B, and NF-L levels were correlated to patients’ age (Spearman coefficient of 0.79).


NF-L showed poor performances in the early management of mTBI patients. NF-L levels are strongly correlated to neurodegeneration, whether physiological, age-related, or pathological.

Corresponding author: Pr. Damien Bouvier, Service de Biochimie et Génétique Moléculaire, Centre de Biologie, CHU Gabriel Montpied, 58 Rue Montalembert, Clermont-Ferrand, 63000, France, Phone: +33 473754882, Fax: +33 473751855, E-mail:

  1. Research funding: None declared.

  2. Author contributions: SK and CO analyzed and interpreted the data. SK wrote the initial version of the manuscript. DB and VS designed the study and assisted with interpretation of the data and writing of the manuscript. JBBM and JS supervised the trial and data collection. SK, JD, AR, and JP carried out assays. BP provided statistical advice for the study design and analyzed the data. All the authors contributed to revision of the manuscript before submission. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: This research complied with the tenets of the Helsinki Declaration (as revised in 2013), and has received approval by the French Patient Protection Committee (CPP Ile-de-France X) (Reference 52–2019).


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Received: 2022-02-25
Accepted: 2022-04-20
Published Online: 2022-05-05
Published in Print: 2022-07-26

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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