Accessible Requires Authentication Published by De Gruyter October 8, 2011

From “Clinical Proteomics” to “Clinical Chemistry Proteomics”: considerations using quantitative mass-spectrometry as a model approach

Sylvain Lehmann, Pauline Poinot, Laurent Tiers, Christophe Junot, François Becher and Christophe Hirtz

Abstract

Clinical Proteomics biomarker discovery programs lead to the selection of putative new biomarkers of human pathologies. Following an initial discovery phase, validation of these candidates in larger populations is a major task that recently started relying upon the use of mass spectrometry approaches, especially in cases where classical immune-detection methods were lacking. Thanks to highly sensitive spectrometers, adapted measurement methods like selective reaction monitoring (SRM) and various pre-fractionation methods, the quantitative detection of protein/peptide biomarkers in low concentrations is now feasible from complex biological fluids. This possibility leads to the use of similar methodologies in clinical biology laboratories, within a new proteomic field that we shall name “Clinical Chemistry Proteomics” (CCP). Such evolution of Clinical Proteomics adds important constraints with regards to the in vitro diagnostic (IVD) application. As measured values of analytes will be used to diagnose, follow-up and adapt patient treatment on a routine basis; medical utility, robustness, reference materials and clinical feasibility are among the new issues of CCP to consider.


Corresponding author: Sylvain Lehmann, Institut de Recherche en Biothérapie, CHU Montpellier, Hôpital St Eloi, Laboratoire de Biochimie Protéomique Clinique et CCBHM, Université Montpellier 1; INSERM U1040, 80 Avenue Augustin Fliche, Montpellier 34295, France

Received: 2011-7-30
Accepted: 2011-9-20
Published Online: 2011-10-08
Published in Print: 2012-02-01

©2012 by Walter de Gruyter Berlin Boston