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BY-NC-ND 4.0 license Open Access Published by De Gruyter September 7, 2017

Microfluidic system for enhanced cardiac tissue formation

  • Mathias Busek EMAIL logo , Tomasz Kolanowski , Stefan Grünzner , Christian Steinfelder , Kaomei Guan and Frank Sonntag


Hereby a microfluidic system for cell cultivation is presented in which human pluripotent stem cell-derived cardiomyocytes were cultivated under perfusion. Besides micro-perfusion this system is also capable to produce well-defined oxygen contents, apply defined forces and has excellent imaging characteristics. Cardiomyocytes attach to the surface, start spontaneous beating and stay functional for up to 14 days under perfusion. The cell motion was subsequently analysed using an adapted video analysis script to calculate beating rate, beating direction and contraction or relaxation speed.

Published Online: 2017-09-07

©2017 Mathias Busek et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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