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BY 4.0 license Open Access Published by De Gruyter November 26, 2020

Initial study on removing cellular residues from hydrostatic high-pressure treated allogeneic tissue using ultrasound

  • Christoph Drobek , Janine Waletzko , Michael Dau , Bernhard Frerich , Volker Weißmann , Daniela Schwerdt , Stefan Seehafer , Robert Mau , Hermann Seitz , Anika Jonitz-Heincke and Rainer Bader


Hydrostatic high-pressure technology (HHD) devitalizes tissue quickly and gently, without negatively affecting the structural properties. HHD-treated tissues must be cleaned from devitalized cells. A partially automated, gentle, reproducible and timesaving rinsing test setup utilizing ultrasound is demonstrated in this study. The test setup is used to clean HHD-treated bone allografts of tissue residues and prevent microbiological contamination. A rinsing procedure is investigated. Residual DNA content determination is utilized to analyze cleaned bone allograft tissue for rinsing procedure evaluation.

Published Online: 2020-11-26
Published in Print: 2020-09-01

© 2020 by Walter de Gruyter Berlin/Boston

This work is licensed under the Creative Commons Attribution 4.0 International License.

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