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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access July 3, 2015

Effects of organic compounds on the macroalgae culture of Aegagropila linnaei

Beata Messyasz , Boguslawa Leska , Joanna Fabrowska , Marta Pikosz , Adam Cieslak and Grzegorz Schroeder
From the journal Open Chemistry


The effects of the impact of four organic compounds (ascorbic acid, biotin, glucose and sucrose) on ash, protein, fiber, fat and amino acid contents in the freshwater Aegagropila linnaei biomass were examined in 7 and 14 days of cultivations in high concentrations of tested compounds (100 mg L-1). The presence of examined organic compounds had a negligible effect on the development of algae and their biomass composition. There were no significant differences in the amino acids composition in the biomass in the presence of organic compounds compared to the test system. However, the increase in ash content was observed irrespective of the cultivation time in the case of all used organic compounds. Only slight differences in crude fat concentration were observed in the case of 7 days cultivation with ascorbic acid, biotin and sucrose, while the highest increase of ash content was observed after 14 days of supplementation with glucose. None of the compounds affected changes in amino acid content in the Aegagropila linnaei biomass. The results suggest that an environment enriched with the test organic compounds had only minimal, or at most short-term, effects on the algal biomass composition.

Graphical Abstract


[1] Crawford R.L., Crawford D.L., Bioremediation: principles and applications. Cambridge University Press, New York, 1996. 10.1017/CBO9780511608414Search in Google Scholar

[2] Jasrotia S., Kansal A., Kishore V.V.N., Arsenic phyco-remediation by Cladophora algae and measurement of arsenic speciation and location of active absorption site using electron microscopy, Microch. Journal, 2014, 114, 197-202. 10.1016/j.microc.2014.01.005Search in Google Scholar

[3] Maznah W.O.W., Al-Fawwaz A.T., Surif M., Biosorption of copper and zinc by immobilised and free algal biomass, and the effects of metal biosorption on the growth and cellular structure of Chlorella sp. and Chlamydomonas sp. isolated from rivers in Penang, Malaysia, J. of Environ. Scien., 2012, 24, 1386-1393. 10.1016/S1001-0742(11)60931-5Search in Google Scholar

[4] Parameswari E., Lakshmanan A., Thilagavathi T., Phyco-remediation of heavy metals in polluted water bodies, Electronic J. of Environ., Agricul. and Food Chem., 2010, 9, 808-814. Search in Google Scholar

[5] Fabrowska J., Łęska B., Algae and their chelating properties, In: Rybachenko V.I. (Ed.), From molecules to functional architecture, Supramolecular interactions. East Publisher House, Donetsk, 2012. Search in Google Scholar

[6] Baran A., Baysal S.H., Sukatar A., Removal of Cr6+ from aqueous solution by some algae, J. of Environ. Biol., 2005, 26, 329-333. Search in Google Scholar

[7] Romera E., Gonzalez F., Ballester A., Blazquez M.L., Munoz J.A., Comparative study of biosorption of heavy metals using different types of algae, Biores. Technol., 2007, 98, 3344-3353. 10.1016/j.biortech.2006.09.026Search in Google Scholar

[8] Rybak A., Messyasz B., Łęska B., Freshwater Ulva (Chlorophyta) as a bioaccumulator of selected heavy metals (Cd, Ni and Pb) and alkaline earth metals (Ca and Mg), Chemosphere, 2012, 89, 1066-1076. 10.1016/j.chemosphere.2012.05.071Search in Google Scholar

[9] Wang J., Chen C., Biosorbents for heavy metals removal and their future, Biotech. Advan., 2009, 27, 195-226. 10.1016/j.biotechadv.2008.11.002Search in Google Scholar

[10] Lee Y.C., Chang S.P., The biosorption of heavy metals from aqueous solution by Spirogyra and Cladophora filamentous macroalgae, Biores. Techn., 2011, 102, 5297-5304. 10.1016/j.biortech.2010.12.103Search in Google Scholar

[11] Rangsayatorn N., Upatham E.S., Kruatrachue M., Pokethitiyook P., Lanza G.R., Phytoremediation potential of Spirulina (Arthrospira) platensis: biosorption and toxicity studies of cadmium, Environ. Poll., 2002, 119, 45-53. 10.1016/S0269-7491(01)00324-4Search in Google Scholar

[12] Sternberg S.P.K., Dorn R.W., Cadmium removal using Cladophora in batch, semi-batch and flow reactors, Biores. Techn., 2002, 81, 249-255. 10.1016/S0960-8524(01)00131-6Search in Google Scholar

[13] Tien C.J., Biosorption of metal ions by freshwater algae with different surface characteristics, Process Biochemistry, 2002, 38, 605-613. 10.1016/S0032-9592(02)00183-8Search in Google Scholar

[14] Tuzen M., Sari A., Biosorption of selenium from aqueous solution by green algae (Cladophora hutchinsiae) biomass: Equilibrium, thermodynamic and kinetic studies, Chem. Engin. J., 2010, 158, 200-206. 10.1016/j.cej.2009.12.041Search in Google Scholar

[15] Laliberte G., Olguin E.J., De La Noue J., Mass cultivation and wastewater treatment using Spirulina. In: Vonshak A. (Ed.). Spirulina platensis (Arthrospira): Physiology, Cell Biology and Biotechnology. Taylor and Francis, London, Bristol and PA, 1997. Search in Google Scholar

[16] Rybak A., Messyasz B., Łęska B., Pikosz M., Fabrowska J., Wydajność asymilacji azotu na przykładzie wybranych gatunków roślin wodnych. In: Schroeder G., Grzesiak P. (Ed.). Środowisko i Przemysł, Cursiva, Poznań, 2012. Search in Google Scholar

[17] Semple K.T., Cain R.B., Schmidt S., Biodegradation of aromatic compounds by microalgae, FEMS Microbiol. Lett., 1999, 170, 291-300. 10.1111/j.1574-6968.1999.tb13386.xSearch in Google Scholar

[18] Katagi T., Bioconcentration, bioaccumulation, and metabolism of pesticides in aquatic organisms, Rev Environ Contam. Toxicol. 2010, 204, 1-132. Search in Google Scholar

[19] Jin Z.P., Luo K., Zhang S., Zheng Q., Yang H. Bioaccumulation and catabolism of prometryne in green algae, Chemosphere, 2012, 87, 278-284. 10.1016/j.chemosphere.2011.12.071Search in Google Scholar PubMed

[20] Andersen R.A., Algal culturing techniques. Elsevier Academic Press, London, 2005. Search in Google Scholar

[21] AOAC, Horwitz W., Latimer W., Association of Official Analytical Chemists, Official Methods of Analysis, 18th Edition, Gaithersburg Maryland, USA, 2007. Search in Google Scholar

[22] Khuantrairong T., Traichaiyaporn S., The nutritional value of edible freshwater alga Cladophora sp. (Chlorophyta) grown under different phosphorus concentrations, Int J. Agric. Biol. 2011, 13, 297-300. Search in Google Scholar

[23] Khuantrairong T., Traichaiyaporn S., Enhancement of carotenoid and chlorophyll content of an edible freshwater alga (Kai: Cladophora sp.) by supplementary inorganic phosphate and investigation of its biomass production, Maejo. Int. J. Sci. Technol., 2012, 6, 1-11. Search in Google Scholar

[24] Kong W-B., Yang H., Cao Y-T., Song H., Hua S-F, Xia C-G., Effect of glycerol and glucose on the enhancement of biomass, lipid and soluble carbohydrates production by Chlorella vulgaris in mixotrophic culture, Food Technol. Biotechnol., 2013, 51, 62-69. Search in Google Scholar

[25] Bhatnagar A., Chinnasamy S., Singh M., Das K.C., Renewable biomass production by mixotrophic algae in the presence of various carbon sources and wastewaters, Appl. Energy, 2011, 88, 3425-3431. 10.1016/j.apenergy.2010.12.064Search in Google Scholar

[26] Rani G., Changes in protein profile and amino acids in Cladophora vagabunda (Chlorophyceae) in response to salinity stress, J. Appl. Phycol., 2007, 19, 803-807. 10.1007/s10811-007-9211-6Search in Google Scholar

[27] Berman T., Chava S., Algal growth on organic compounds as nitrogen sources, J. Plankton Res. 1999, 21, 1423-1437. Search in Google Scholar

Received: 2015-2-12
Accepted: 2015-5-15
Published Online: 2015-7-3

© 2015 Beata Messyasz et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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