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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access August 20, 2015

FT-Raman spectroscopy as a tool in evaluation the response of plants to drought stress

Magdalena Rys, Maciej Szaleniec, Andrzej Skoczowski, Iwona Stawoska and Anna Janeczko
From the journal Open Chemistry


The aim of study was to evaluate the usefulness of FT-Raman spectroscopy in assessing stress-induced metabolic changes in plants. 20-d-old optimally watered plants of soybean were exposed to drought. Metabolic changes in optimally watered and drought-stressed plants were monitored using FT-Raman spectroscopy. In parallel, analyses were carried out of fatty acid composition and pigment content using analytical methods. These compounds are associated with the response of plants to environmental stress. While fatty acid assays in study were inconclusive, the pigment content analysis gave promising results. FT-Raman experiment demonstrated a decrease in carotenoid content in leaf, as a result of drought, which was confirmed by spectrophotometric analysis. In addition to the analysis of aforementioned compounds, FT-Raman spectroscopy allowed the simultaneous observation of a wider spectrum of compounds scattering the radiation in the leaves tested, and their subsequent comparative analysis. The impact of drought on metabolism of soybean was clearly visible on spectra and confirmed using cluster analysis. The technical problem of the influence of leaf water content on measurements, which appeared in studies, will be discussed. To conclude, FT-Raman spectroscopy may be a good complement to other non-invasive methods, e.g., fluorescent methods, in assessing the stress-induced damage of crops.


[1] P. Rodziewicz, B. Swarcewicz, K. Chmielewska, A. Wojakowska, M. Stobiecki, Acta Physiol. Plant. 36, 1 (2014) 10.1007/s11738-013-1402-ySearch in Google Scholar

[2] T. Hájek, L. Adamec, Biologia 65, 69 (2010) 10.2478/s11756-009-0211-7Search in Google Scholar

[3] J. Peñuelas, I. Filella, Trends Plant Sci. 3, 151 (1998) 10.1016/S1360-1385(98)01213-8Search in Google Scholar

[4] A. Janeczko, J. Oklešťková, A. Siwek, M. Dziurka, E. Pociecha, M. Kocurek, O. Novák, J Steroid Biochem. Mol. Biol. 138, 384 (2013) 10.1016/j.jsbmb.2013.07.014Search in Google Scholar

[5] B.N. Smith, R.S. Criddle, L.D. Hansen, J. Plant Biol. 27, 89 (2000) Search in Google Scholar

[6] A. Skoczowski, M. Troć, Isothermal calorimetry and Raman spectroscopy to study response of plants to abiotic and biotic stress. In: Molecular Stress Physiology of Plants (Springer, 2013) 10.1007/978-81-322-0807-5_11Search in Google Scholar

[7] C. Frankenberg, J. Berry, L.Guanter, J.Joiner, SPIE Newsroom: 10.1117/2.1201302.004725. (2013) Search in Google Scholar

[8] B. Schrader, H. Schulz, G.N. Andreev, H.H. Klump, J. Sawatzki, Talanta 53, 35 (2000) 10.1016/S0039-9140(00)00385-4Search in Google Scholar

[9] H. Schulz, M. Baranska, Vib. Spectrosc. 43, 13 (2007) 10.1016/j.vibspec.2006.06.001Search in Google Scholar

[10] N. Gierlinger, M. Schwanninger, Spectroscopy 21, 69 (2007) 10.1155/2007/498206Search in Google Scholar

[11] R.S. Das, Y.K. Agrawal, Vib. Spectrosc. 57, 163 (2011) 10.1016/j.vibspec.2011.08.003Search in Google Scholar

[12] M. Troć, A. Skoczowski, M. Barańska, J. Therm. Anal. Calorim. 95, 727 (2009) 10.1007/s10973-008-9408-1Search in Google Scholar

[13] A. Skoczowski, M. Troć, A. Baran, M. Baranska, J. Therm. Anal. Calorim. 104, 187 (2011) 10.1007/s10973-010-1225-7Search in Google Scholar

[14] K.S. Liu, Chemistry and nurtitional value of soybean components. In Soybean: Chemistry, Technology, and Utilization (Chapman & Hall, New York, 1997) 10.1007/978-1-4615-1763-4_2Search in Google Scholar

[15] Z. Wu, D. Schenk-Hamlin, W. Zhan, D.W. Ragsdale, G.E. Heimpel, Ann. Entomol. Soc. Am. 97, 209 (2004) 10.1093/aesa/97.2.209Search in Google Scholar

[16] S. Munné-Bosch, L. Alegre, Planta, 210, 925 (2000) 10.1007/s004250050699Search in Google Scholar

[17] A.K. Parida, V.S. Dagaonkar, M.S. Phalak, G.V. Umalkar, L.P. Aurangabadkar, Plant Biotechnol. Rep. 1, 37 (2007) 10.1007/s11816-006-0004-1Search in Google Scholar

[18] A. Janeczko, K. Hura, A. Skoczowski, I. Idzik, J. Biesaga-Kościelniak, E. Niemczyk, Acta Physiol. Plant. 31, 71 (2009) 10.1007/s11738-008-0202-2Search in Google Scholar

[19] H.K. Lichtenthaler, A.R. Wellburn, Biochem. Soc. T. 603, 590 (1983) Search in Google Scholar

[20] X. Xiao, X. Xu, F. Yang, Silva Fenn. 42, 705 (2008) 10.14214/sf.224Search in Google Scholar

[21] R. Withnall, B.Z. Chowdhry, J. Silver, H.G.M. Edwards, L.F.C. de Oliveira, Spectrochim. Acta A, 59, 2207, (2003) 10.1016/S1386-1425(03)00064-7Search in Google Scholar

[22] R. Baranski, M. Baranska, H. Schulz, Planta 222, 448 (2005) 10.1007/s00425-005-1566-9Search in Google Scholar PubMed

[23] M. Baranska, H. Schulz, E. Joubert, M. Manley, Anal. Chem. 78, 7716 (2006) 10.1021/ac061123qSearch in Google Scholar PubMed

[24] M. Baranska, R. Baranski, H. Schulz, T. Nothnagel, Planta 224, 1028, (2006) 10.1007/s00425-006-0289-xSearch in Google Scholar PubMed

[25] E.H. Papaioannou, M. Liakopoulou-Kyriakides, D. Christofilos, I. Arvanitidis, G. Kourouklis, Appl. Biochem. Biotechnol. 159, 478 (2009) 10.1007/s12010-008-8472-0Search in Google Scholar PubMed

[26] A. Janeczko, J. Biesaga-Kościelniak, M. Dziurka, J. Oklešťková, M. Kocurek, G. Szarek-Łukaszewska, Z. Janeczko, Acta Sci. Pol. (Agricultura) 10, 33 (2011) 10.1007/s11738-010-0655-ySearch in Google Scholar

[27] I. Toumi, M. Gargouri, I. Nouairi, P.N. Moschou, A. Ben Salem-Fnayou, A. Mliki, M. Zarrouk, A. Ghorbel, Biol. Plantarum 52, 161 (2008) 10.1007/s10535-008-0035-2Search in Google Scholar

[28] A. Gigon, A.R. Matos, D. Laffray, Y. Zuily-Fodil, A.T. Pham-Thi, Ann. Bot. 94, 345 (2004) 10.1093/aob/mch150Search in Google Scholar PubMed PubMed Central

[29] D. Zhong, H. Du, Z. Wang, J. Am. Soc. Hortic. Sci. 136, 35 (2011) 10.21273/JASHS.136.1.35Search in Google Scholar

[30] F.I. Collins, V.E. Sedgwick, J. Am. Oil Chem. Soc. 36, 641 (1959) 10.1007/BF02640276Search in Google Scholar

[31] N. Bellaloui, Am. J. Plant Sci. 2, 692 (2011) 10.4236/ajps.2011.25084Search in Google Scholar

[32] M.G. Kleinschmidt, V.A. McMahon, Plant Physiol. 46, 286 (1970) 10.1104/pp.46.2.286Search in Google Scholar PubMed PubMed Central

[33] M. Żuk, L. Dymińska, A. Kulma, A. Boba, A. Prescha, J. Szopa, M. Mączka, A. Zając, K. Szołtysek, J. Hanuza, Spectrochim. Acta A 78,1080 (2011) 10.1016/j.saa.2010.12.054Search in Google Scholar PubMed

[34] M. Troć, Impact of herbal extract with different allelopathic potential on metabolism of mustard, rape, wheat and clover seedlings. PhD Thesis. (Krakow, in Polish, 2011) Search in Google Scholar

Received: 2014-8-26
Accepted: 2015-6-1
Published Online: 2015-8-20

© 2015 Magdalena Rys et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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