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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access November 25, 2015

Analysis of oligonucleotides by liquid chromatography with alkylamide stationary phase

  • Bogusław Buszewski , Zahra Safaei and Sylwia Studzińska
From the journal Open Chemistry


The main aim of the present investigation was to determine retention behavior and interactions of oligonucleotides with alkylamide stationary phase. Five oligonucleotides, differing in the sequence, were tested. Changes in the composition of the mobile phase, i.e. pH (5.0−7.0) and buffer concentration (30−75 mM) were investigated in detail. In addition, the hydrophobic and electrostatic parameters were measured for the different pH’s and salt concentrations. The theoretical model of interactions between individual elements of the separation system, (e.g. solute, stationary phase, and mobile phase) based on zeta potential measurements, hydrophobic and electrostatic parameters calculation, and molecular modeling, has been considered.

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Received: 2015-1-16
Accepted: 2015-9-14
Published Online: 2015-11-25

© 2015 Bogusław Buszewski et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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