Skip to content
BY 4.0 license Open Access Published by De Gruyter Open Access May 8, 2023

Physicochemical and biological properties of carvacrol

  • Vanya Gandova , Anton Lazarov , Hafize Fidan , Milen Dimov , Stanko Stankov , Petko Denev , Sezai Ercisli , Albena Stoyanova , Hatice Gulen , Amine Assouguem EMAIL logo , Abdellah Farah , Riaz Ullah , Mohammed Kara and Ahmed Bari
From the journal Open Chemistry


Carvacrol is a major component of many essential oils of the genus Thymus, Satureja, and Origanum, determining their antimicrobial, antioxidant, and other properties. The aim of the present study was to investigate some physicochemical and biological properties of pure carvacrol. The surface tension and density were measured at six different temperatures (30, 40, 50, 60, 70, and 80°C). The surface tension values were between 53.11 and 60.38 mN/m, while density varied from 0.978 to 0.99 kg/m3. The antibacterial activity against seven pathogenic and conditionally pathogenic bacteria was investigated. The diameter of the inhibition zones was determined in the range of 3.9–4.9 mm. The antioxidant activity was determined by the oxygen radical absorbance capacity (1687.0 µmol TE/mL) method.

1 Introduction

Carvacrol (2-methyl-5-isopropyl phenol, 2-p-cymenol, 2-hydroxy-p-cymene, isopropyl-o-cresol, isothymol: molecular weight of 150.22 [CAS 499-75-2]). Its properties are as follows: thick colorless to pale yellow liquid with pungent, spicy odor; relative density ( d 20 20 ) 0.974–0.979; refractive index ( n D 20 ), 1.5210–1.5260; melting point, 1°C; boiling point, 237–238°C; slightly soluble in water; soluble in ethanol, ether, alkalis; and very soluble in water [1,2].

This aromatic substance is mainly found in genera Thymus, Satureja, and Origanum plants, which belong to the family Lamiaceae. In case of improper storage of these essential oils (high temperature, presence of water, light, etc.), color changes occur due to separating precipitates from phenolates. It is known that in the presence of iron, phenols are oxidized in order to form red-colored products. It has been shown that hydroquinone is initially produced and oxidized to quinone, followed by polymerization processes. With prolonged exposure, the color changes to red-brown may occur. Oxidation is accompanied not only by a change in color but also by a change in smell. Initially, hydroperoxides are formed. Later, they undergo various transformations, turning into terpineol, carvone, polyhydroxy compounds, and finally into high-molecular polymer compounds [1,2].

Carvacrol is used in perfumery and cosmetics [1,2], as well as for the preparation of racemic menthol [1].

As a phenol, carvacrol possesses highly pronounced antimicrobial [3,4,5,6,7,8,9,10], antioxidant [11,12,13], and other biological properties [14,15].

Density, viscosity, refractive index, and surface tension in water/alcohol systems were measured and found that pure alcohols or alcohols mixed with water were used as solvents in industrial applications of pharmaceutical, perfumery, and cosmetic products [16]. Surface and interfacial tension against water for mint, lemon, and oregano oils were investigated as a function of time and temperature [17]. A mix of linalool + propan-1-ol was investigated in a temperature range between 283.15 and 328.15 K. Refractive index of the mixture was measured and determined that the essential oils are sensitive to light [18]. Density, surface tension, and refractive index were determined for investigation of equilibrium in the system 1-pentanol-R-(+)-limonene in temperature range [19]. Linear dependence between density and refractive indexes of different aromatic compounds down to the temperature of 273 K was investigated [20].

The aim of the present study was to investigate carvacrol as an aromatic substance by determining some physicochemical parameters, antibacterial and antioxidant activities, looking for possibilities for its application, as well as the essential oils containing it in food and cosmetic products.

2 Materials and methods

2.1 Material

Carvacrol was delivered from Sigma Aldrich, USA.

2.2 Physical and chemical indexes

The physical indicators of the refractive index [21] and relative density [22] have been determined. The infrared spectrum of carvacrol was recorded using a Nicolet iS 50 Thermo Scientific FT-IR spectrometer in the frequency region of 4,000–400 cm–1, with the sample embedded in KBr matrixes.

Surface tension is determined by equation (1) [23].

(1) y = rg 2 ( Δ H ρ 0 r ρ ) ,

where r is the radius of the capillary (m), g is the acceleration of gravity (m/s2), ΔH is the maximum difference in the two gauges of the gauge, and ρ 0, ρ is the density of the manometric (water) and test liquid (kg/m3).

The density of the test liquid is determined by equation (2) [23].

(2) ρ = m 1 m V = m 1 m m 1 m ρ 1 = m 1 m m 2 m ρ 1 ,

where ρ is the density of the carvacrol (kg/m3), ρ 1 is the density of distilled water (kg/m3), m is the mass of the pycnometer (g), m 1 is the mass of the pycnometer with the carvacrol (g), and m 2 is the mass of the pycnometer with distilled water (g).

These two physicochemical indicators were measured at six temperatures (30, 40, 50, 60, 70, and 80°C), which were most often used in applying aromatic substances in various food and cosmetic products.

2.3 Antibacterial activity

Test microorganisms. Strains of pathogenic bacteria, reported as causing infections, toxicoinfections, and toxicosis, were used as test microorganisms. The investigated test cultures were some of the most common, both on the surface of food and cosmetic preparations, on work surfaces in companies, and on the hands of staff. Test microorganisms strains were supplied by the National Bank for Industrial Microorganisms and Cell Cultures, except for two that are clinical isolates. The following Gram-positive bacteria were used in this study: Listeria monocytogenes NCTC 11994, Staphylococcus aureus ATCC 25093, Bacillus subtilis ATCC 6633, and Gram-negative bacteria – Escherichia coli ATCC 8739, Salmonella enterica subsp. Enterica serovar Abony NCTC 6017.

The Gram-positive bacterium Bacillus cereus and the Gram-negative bacterium Klebsiella sp. are clinical isolates from the University of Food Technologies collection.

Antibacterial activity was determined by modifying the agar diffusion method by measuring the inhibition zones of the pathogen (1.104 cfu/mL) growth around metal rings (Ø = 6 mm) [24].

2.4 Antioxidant activity

The lipophilic oxygen radical absorbance capacity assay measures the antioxidant scavenging function of lipophilic antioxidants against peroxyl radical induced by 2,2′-azobis(2-amidinopropane) dihydrochloride at 37°C [25].

2.5 Statistical analysis

The measurements were performed in triplicate. The results are presented as a mean value of the individual measurements with the corresponding standard deviation (SD).

3 Results and discussion

3.1 Physical and chemical indexes

Carvacrol is a clear yellowish liquid with a specific odor and the following physical indicators: relative density ( d 20 20 ) 0.980 ± 0.0 and refractive index ( n D 20 ) 1.524 ± 0.01. The values of the physical parameters of the studied carvacrol sample do not differ from data in the literature [1,2].

The IR spectrum of carvacrol is shown in Table 1 and Figure 1. In the carvacrol spectrum, clear characteristic bands of absorption were observed at 3,385 cm−1, which was characteristic of γOH group associated with the aromatic ring. The band at 3,021 cm−1 was characteristic of the γC–H relationship characteristic of an aromatic ring. The band at 2,961 cm−1 was characteristic of the γ as CH3 group, which was confirmed by an additional band at 2,870 cm−1. Also noticeable was an absorption band at 2,927 cm−1, which was characteristic of the γ as –CH2– group. The absorption band at 1,621 cm−1 was characteristic of an absorption band of a conjugate double C═C bond. The intense absorption band at 1,589 cm−1 was characteristic of the carbon skeleton with an aromatic structure that was more intense about 1,500 cm−1 (in this case 1,502 cm−1). The bands 1,589, 1,522, 1,502, 1,459, and 1,421 cm−1 were characteristic of the aromatic ring, with the strip 1,421 cm−1 being characteristic of the −C–O–H bond in phenols. The bands 1,117, 995, and 866 cm−1 were characteristic of aromatic rings with substituents in positions 1, 2, and 4. The band at 813 cm−1 was typical for a p-substituted aromatic ring and that at 757 cm−1 for an o-substituted ring. From the conclusions drawn, it could be concluded that this compound belonged to the phenol group which had a CH3 group in the o-position and a group of the type C(CH3)2 in the p-position, which was confirmed by the structural formula of carvacrol [26,27].

Table 1

IR Spectrum of carvacrol

Characteristic bands (cm−1) Group type
Carvacrol Reference data
3,385 3,470–3,322 γOH group to aromatic ring (phenol)
3,021 3,040–3,010 γ C–H average absorption band, characteristic of aromatics
2,961 2,970–2,950 Linear γ as CH3
2,927 2,940–2,915 γ as –CH2
2,870 2,885–2,860 Presence of CH3 group
2,870–2,845 Highly pronounced band γ asCH2
1,621 1,680–1,620 A little intense band of absorption, conjugated C═C
1,589 1,600–1,575 Pulsation oscillations of the carbon skeleton at an aromatic structure more intense about 1,500 cm−1 (in this case 1,502 cm−1)
1,522 1,525–1,475 Oscillations of substituents at aromatic ring
1,502 intense about 1,500 cm−1 Pulsation oscillations of the carbon skeleton at aromatic ring
1,459 1,465–1,440 Characteristic band of aromatic ring
1,421 1,435–1,415 −C–O–H characteristic band of phenol
1,362 1,370–1,365 Two bands of approximately the same intensity corresponding to a structural fragment of the type C(CH3)2
1,302 1,310–1,290 Fluctuations δc-n of connection C–H at the end vinyl group or associated with an aromatic ring
1,175 1,175–1,165 A characteristic band of the type CH3–CH–CH3
1,117 1,125–1,090 This is 1, 2, 4 substituted aromatics
995 1,000–960 This is 1, 2, 4 substituted aromatics
937 950–860 Antisymmetric valence oscillations of a ring
866 900–865 Three substituted aromatic ring at position 1, 2, 4
813 855–800 p-Substituted aromatic ring
757 770–735 o-Substituted aromatic ring
Figure 1 
                  FT-IR Spectrum of carvacrol.
Figure 1

FT-IR Spectrum of carvacrol.

The surface tensions and density of pure carvacrol were measured. The results are presented in Table 2. The data showed that as the temperature increased, the values of surface tension and density decreased is a classical thermodynamic dependence and does not contradict the literature for other substances [28]. The carvacrol usually had less tension than the water (72.75 mN/m) [29]. The decrease in the value of surface tension with increasing temperature is smooth, as at 40°C it is only 0.04%, at 50°C – by 2.42%, at 60°C – by 7.24%, at 70°C – by 9.64%, and at 80°C – by 12.04%, and the dependence is considered linear. A similar trend is found in the density, as the temperature dependence is also linear – at 40°C, the decrease is by 0.30%, at 50°C – by 0.50%, at 60°C – by 0.80%, at 70°C – by 1.00%, and at 80°C – with 1.31%.

Table 2

Surface tension and density at different temperatures

Temperature, °C Surface tension, mN/m Density, kg/m3
30 60.38 ± 0.08 0.991 ± 0.11
40 60.35 ± 0.11 0.988 ± 0.08
50 58.92 ± 0.12 0.986 ± 0.17
60 56.01 ± 0.06 0.983 ± 0.03
70 54.56 ± 0.16 0.981 ± 0.11
80 53.11 ± 0.03 0.978 ± 0.08

When increasing the temperature from 30 to 80°C, no change in the color and smell of carvacrol was detected, indicating that oxidation and polymerization processes did not occur. Our results did not differ from the data reported by Fonseca et al. [27], according to which changes in the structure of carvacrol may occur towards 160°C. The temperature stability of carvacrol was explained by the presence of the aromatic ring [26,27].

In the literature, there are data on values of these physicochemical parameters at different temperatures. Still, for solutions of linalool [18,30] and other aromatic substances [19,20,31] in ethanol, propanol, and other solvents, it is not possible to make a comparison.

3.2 Antibacterial activity

The antibacterial activity of carvacrol is presented in Table 3. The data showed that the diameter of the zones of inhibition was in the range of 3.9–4.9 mm. The antimicrobial action of carvacrol has been found to be due to: depletion of intracellular ATF [10], induction of reactive oxygen species [32], inhibition of efflux pump [33], and inhibition of bacterial biofilm [34].

Table 3

Antimicrobial activity of carvacrol

Test-microorganisms Diameter of zone of inhibition, mm
Gram-positive bacteria
Listeria monocytogenes NCTC 11994 4.2 ± 0.04
Staphylococcus aureus ATCC 25093 4.0 ± 0.03
Bacillus subtilis ATCC 6633 3.9 ± 0.03
Bacillus cereus (clinical isolate) 3.7 ± 0.03
Gram-negative bacteria
Escherichia coli ATCC 8739 4.5 ± 0.04
Salmonella enterica subsp. Enterica serovar Abony NCTC 6017 4.9 ± 0.04
Klebsiella (clinical isolate) 3.9 ± 0.03

The data presented in Table 3 showed that no differejnce in antibacterial activity was reported against Gram-positive and Gram-negative bacteria. The established differences in the studied antibacterial activity and data from the literature, both for the pure substance and for the essential oils that contain it, could be explained by the analysis methods used in our study. In our research, “wells” were used into which carvacrol was dripped. In other studies published in the literature, “filter discs” were used, onto which the carvacrol was put. The aromatic substance’s concentration and the tested bacteria’s suspension should also be considered.

For example, Sarrazin et al. [35] reported that carvacrol was active against Gram-positive Bacillus cereus, B. subtilis, and Gram-negative bacteria Salmonella typhimurium, which was highly related to its chemical structure and composition. In another study, Du et al. [36] reported strong antibacterial activity of the carvacrol against pathogenic Gram-negative bacteria Escherichia coli, Clostridium perfringens, Salmonella strains, and Gram-positive Lactobacillus strains. Our results were lower than that reported by Bnyan et al. [37] who investigated the antimicrobial effects of carvacrol against nine microbial species. As a result, they reported that carvacrol showed maximum inhibition against E. coli (26 mm), Klebsiella pneumonia (23 mm), and S. aureus (20 mm). The differences in the results could be due to the used species and the differences in the applied bacterial concentrations.

3.3 Antioxidant activity

The value for the antioxidant effect of carvacrol, determined by the oxygen radical absorbance capacity (ORAC) method described in the study was 1687.0 ± 102.90 µmol TE/mL. ORAC has some advantages that made it one of the most widely used methods for estimating total antioxidant capacity. It is based on the generation of peroxyl radicals that are relevant in food and biological systems. Additionally, it is performed under physiological pH (7.4) and temperature (37°C) and most importantly, the assay has a modification for lyphophilic antiradical activity, in which lypophilic components are dissolved in water/buffer media with randomly methylated β-cyclodextrins. Essential oils containing carvacrol were known to exhibit antioxidant activity [12,14,38,39], which the authors attribute to its presence. The antioxidant activity of carvacrol was determined by different methods by a number of authors: ORAC (2.8 µmol Trolox equivalent/µL), ABTS (7804.4 µmol Trolox equivalent/µL), DPPH (activity lower than 0.05 µmol Trolox equivalent/µL), chelating power (activity lower than 0.05 μmol ethylenediaminetetraacetic acid equivalent/mL), and potassium ferricyanide (3239.6 μmol ascorbic acid equivalent/L) [40], DPPH (IC50 448.05 (lg/mL [41], DPPH (79.75 μg/mL) [42] and ORAC (152.23 μmol Trolox equivalent/g) and DPPH (51.09 %) [43]. The different values could be explained by the analysis methods used.

Other data on the antioxidant activity of essential oils containing carvacrol, determined by the described method, were not found in the literature. It is not easy to make a more detailed comparison.

4 Conclusions

Some physicochemical parameters at six different temperatures and two biological properties of pure carvacrol were investigated. Weak antimicrobial activity was determined against seven pathogenic and conditionally pathogenic microorganisms, and the ORAC method was used in order to determine the antioxidant activity. The results showed that carvacrol, as well as essential oils containing it in larger quantities, could be used as an independent ingredient in food and cosmetic formulations, which could be a subject of further studies.


The authors extend their appreciation to the researchers supported project number (RSP2023R346) at King Saud University, Riyadh, Saudi Arabia, for financial support.

  1. Funding information: Authors wish to thanks Researchers Supporting Project Number (RSP2023R110) at King Saud University Riyadh Saudi Arabia for financial support.

  2. Author contributions: Conceptualization, V.G.; A.S. and S.E.; formal analysis, H.F.; S.S.; M.D., P.D. and A.B.; writing – original draft preparation, V.G.; H.F; S.S.; A.S.; S.E. and H.G.; writing – review and editing, H.F., A.S.; S.E. and R.U.; supervision, H.F.; S.S.; A.S.; S.E. and A.B. All authors have read and agreed to the published version of the manuscript.

  3. Conflict of interest: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

  4. Ethical approval: The conducted research is not related to either human or animal use.

  5. Data availability statement: The data presented in this study are available on request from the authors.


[1] Bauer K, Garbe D, Surburg H. Common fragrance and flavor materials. Preparation, properties and uses. Fourth completely revised edition. Weinheim, New York, Chichester, Brisbane, Singapore, Toronto: Wiley – VCH; 2001. 10.1002/3527608214.Search in Google Scholar

[2] Stoyanova A. A Guide for the Specialist in the Aromatic Industry. Bulgarian National Association of Essential Oils, Perfumery and Cosmetics. Plovdiv. Bulgaria; 2022.Search in Google Scholar

[3] Jirovetz L, Wlcek K, Buchbauer G, Gochev V, Girova T, Stoyanova A, et al. Antifungal activity of various lamiaceae essential oils rich in thymol and carvacrol against clinical isolates of pathogenic Candida species. Int J Essent Oil Ther. 2007;1:153–7.Search in Google Scholar

[4] Kim N, Moon H, Rhee M. Sodium chloride significantly enhances the bactericidal actions of carvacrol and thymol against the halotolerant species Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus 109015. LWT. 2007;122. 10.1016/j.lwt.2020.109015.Search in Google Scholar

[5] Lee J, Kim Y, Lee J. Carvacrol-rich oregano oil and thymol-rich thyme red oil inhibit biofilm formation and the virulence of uropathogenic Escherichia coli. J Appl Microbiol. 2017;123(6):1420–8. 10.1111/jam.13602.Search in Google Scholar PubMed

[6] Marchese A, Arciola C, Coppo E, Barbieri R, Barreca D, Chebaibi S, et al. The Natural plant compound carvacrol as an antimicrobial and anti-biofilm agent: mechanisms, synergies and bio-inspired anti-infective materials. Biofouling. 2018;34(6):630–56. 10.1080/08927014.2018.1480756.Search in Google Scholar PubMed

[7] Rua J, Del Valle P, De Arriaga D, Fernandez-Alvarez L, Garcia-Armesto M. Combination of carvacrol and thymol: Antimicrobial activity against Staphylococcus aureus and antioxidant activity. Foodborne Pathog Dis. 2018;6(9):622–9. 10.1089/fpd.2018.2594.Search in Google Scholar PubMed

[8] Sim J, Khazandi M, Chan W, Trott D, Deo P. Antimicrobial activity of thyme oil, oregano oil, thymol and carvacrol against sensitive and resistant microbial isolates from dogs with Otitis externa. Vet Dermatol. 2019;30(6):524–e159. 10.1111/vde.12794.Search in Google Scholar PubMed

[9] Veldhuizen E, Tjeerdsma-van Bokhoven J, Zweijtzer C, Burt S, Haagsman H. Structural requirements for the antimicrobial activity of carvacrol. J Agric Food Chem. 2006;54:1874–9. 10.1021/jf052564y.Search in Google Scholar PubMed

[10] Zhang D, Gan R, Ge Y, Yang Q, Ge J, Li H, et al. Research Progress on the antibacterial mechanisms of carvacrol: A Mini review. Bioact Compd Health Dis. 2018;1(6):71. 10.31989/bchd.v1i6.551.Search in Google Scholar

[11] Llana-Ruiz-Cabello M, Gutiérrez-Praena D, Puerto M, Pichardo S, Jos A, Cameán A. In vitro pro-oxidant/antioxidant role of carvacrol, thymol and their mixture in the intestinal Caco-2 cell line. Toxicol Vitr. 2015;29:647–56. 10.1016/j.tiv.2015.02.006.Search in Google Scholar PubMed

[12] Tohidi B, Rahimmalek M, Arzani A, Sabzalian M. Thymol, carvacrol, and antioxidant accumulation in Thymus species in response to different light spectra emitted by light-emitting diodes. Food Chem. 2020;307:125521. 10.1016/j.foodchem.2019.125521.Search in Google Scholar PubMed

[13] Yanishlieva N, Marinova E, Gordon M, Raneva V. Antioxidant activity and mechanisms of action of thymol and carvacrol in two system. Food Chem. 1999;64:59–66. 10.1016/S0308-8146(98)00086-7.Search in Google Scholar

[14] Başer K. Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Curr Pharm Des. 2008;14(29):3106–19. 10.2174/138161208786404227.Search in Google Scholar PubMed

[15] Sharifi-Rad M, Varoni E, Iriti M, Martorell M, Setzer W, Del Mar Contreras M, et al. Carvacrol and human health: a comprehensive review. Phytother Res. 2018;32(9):1675–88. 10.1002/ptr.6103.Search in Google Scholar PubMed

[16] Belda R, Herraez J, Diez O. A Study of the refractive index and surface tension synergy of the binary water/ethanol: influence of concentration. Phys Chem Liq. 2005;43(1):91–101. 10.1080/00319100512331327342.Search in Google Scholar

[17] Arneodo C, Bazkin A, Benoit J, Fellous R, Thies C. Interfacial studies of essential oil-water system. Coll Surf. 1988/89;34:159–69. 10.1016/0166-6622(88)80094-5.Search in Google Scholar

[18] Garcia-Abarrio S, Torcal M, Haya M, Urieta J, Mainar A. Thermophysical properties of {(±)-linalool + propan-1-ol}: A First stage towards the development of a green process. J Chem Thermodyn. 2011;43:527–36. 10.1016/j.jct.2010.11.007.Search in Google Scholar

[19] Clara R, Gomez Marigliano A, Del Campos V, Solimo H. Density, viscosity, vapour–liquid equilibrium, excess molar enthalpy, and their correlations of the binary system [1-pentanol+r-(+)-limonene] over the complete concentration range, at different temperatures. Fluid Ph Equilib. 2010;293:151–6. 10.1016/j.fluid.2010.03.001.Search in Google Scholar

[20] Markarian S, Terzyan A. Surface tension and refractive index of dialkylsulfoxide + water mixtures at several temperatures. J Chem Eng Data. 2007;52(5):1704–9. 10.1021/je7001013.Search in Google Scholar

[21] ISO 280:1998280:1998. Essential oils – Determination of Refractive Index.Search in Google Scholar

[22] ISO 279:1998279:1998. Essential oils – Determination of Relative Density at 20°C – Reference Method.Search in Google Scholar

[23] Yankova R, Gandova V, Dimov M, Dobreva K, Prodanova-Stefanova V, Stoyanova A. Studies on the structural, electronic and physical properties of linalool. Oxid Commun. 2019;42(3):293–306.Search in Google Scholar

[24] Zaika L. Spices and herbs: Their Antimicrobial activity and its determination. J Food Saf. 1988;2:97–118. 10.1111/j.1745-4565.1988.tb00511.x.Search in Google Scholar

[25] Huang D, Ou B, Hampsch-Woodill M, Flanagan J, Deemer E. Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated β-cyclodextrin as the solubility enhancer. J Agric Food Chem. 2002;50(7):1815–21. 10.1021/jf0113732.Search in Google Scholar PubMed

[26] Scaffaro R, Lopresti F. Processing, structure, property relationships and release kinetics of electrospun PLA/Carvacrol membranes. Eur Polym J. 2017;100:165–71. 10.1016/j.eurpolymj.2018.01.035.Search in Google Scholar

[27] Fonseca L, Cruxen C, Bruni G, Fiorentin A, Zavareze E, Lim L-T, et al. Development of antimicrobial and antioxidant electrospun soluble potato starch nanofibers loaded with carvacrol. Int J Biol Macromol. 2019;139:182–1190. 10.1016/j.ijbiomac.2019.08.096.Search in Google Scholar PubMed

[28] Yerima J, Solomon S, Dikko A. Temperature dependence of density and dynamic surface tension of groundnut oil and palm oil. Int J Eng Sci. 2015;4(6):49–55.Search in Google Scholar

[29] Raev N. Matter – Nature, properties and events. Plovdiv: UFT Academic Publishing House; 2008.Search in Google Scholar

[30] Cháfer A, de la Torre J, Muñoz R, Burgue M. Liquid–liquid equlibria of the mixture linalool+ethanol+water at different temperatures. Fluid Phase Equilib. 2005;238:72–6. 10.1016/j.fluid.2005.09.009.Search in Google Scholar

[31] Calvar N, Gonzalez B, Gomez E, Canosa J. Density, speed of sound, and refractive index for binarymixtures containing cycloalkanes and aromatic compounds at T = 313.15K. J Chem Eng. 2009;54:1334–9. 10.1021/je8009336.Search in Google Scholar

[32] Khan I, Bahuguna A, Kumar P, Bajpai V, Kang S. Antimicrobial potential of carvacrol against uropathogenic Escherichia coli via membrane disruption, depolarization, and reactive oxygen species generation. Front Microbiol. 2017;8:2421. 10.3389/fmicb.2017.02421.Search in Google Scholar PubMed PubMed Central

[33] Miladi K, Zmantar T, Chaabouni Y, Fedhila K, Bakhrouf A, Mandouani K, et al. Antibacterial and efflux pump of thymol and carvacrol against food-borne pathogens. Microb Pathog. 2016;99:95–100. 10.1016/j.micpath.2016.08.008.Search in Google Scholar PubMed

[34] Campana R, Baffone W. Carvacrol efficacy in reducing microbial biofilms on stainless steel and in limiting re-growth of injured cells. Food Control. 2018;90:10–7. 10.1016/j.foodcont.2018.02.029.Search in Google Scholar

[35] Sarrazin S, da Silva L, Oliveira R, Raposo J, da Silva J, Salimena F, et al. Antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from Lippia origanoides Kunth. Lipids Health Dis. 2015;14:145. 10.1186/s12944-015-0146-7.Search in Google Scholar PubMed PubMed Central

[36] Du E, Gan L, Li Z, Wang W, Liu D, Guo Y. In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens. J Anim Sci Biotechnol. 2015;6:58. 10.1186/s40104-015-0055-7.Search in Google Scholar PubMed PubMed Central

[37] Bnyan I, Abid A, Obied H. Antibacterial activity of carvacrol against different types of bacteria. J Nat Sci Res. 2014;4:9.Search in Google Scholar

[38] Jelenkovic L, Stankov-Jovanovic V, Palic I, Mitic V, Jevtovic-Stoimenov T, Radulovic M. Examination of the decrease in activity of clinically significant enzymes in commercial serum caused by terpenes in vitro. Oxid Commun. 2016;39(4):3156–67.Search in Google Scholar

[39] Bayraktar O, Yerkesikli A. Oxidation of oregano essential oil using zeolite-encapsulated Cr(SALPU) complex. Oxid Commun. 2017;40(4):1275–81. in Google Scholar

[40] Cutillas A, Carrasco A, Martinez-Gutierrez R, Tomas V, Tudela J. Thyme essential oils from Spain: Aromatic profile ascertained by GC–MS, and their antioxidant, anti-lipoxygenase and antimicrobial activities. J Food Drug Anal. 2018;26:529–44. 10.1016/j.jfda.2017.05.004.Search in Google Scholar PubMed PubMed Central

[41] Safaei-Ghomi J, Ebrahimabadi A, Djafari-Bidgoli Z, Batooli H. GC/MS analysis and in vitro antioxidant activity of essential oil and methanol extracts of Thymus caramanicus Jalas and its main constituent carvacrol. Food Chem. 2009;115:1524–8. 10.1016/j.foodchem.2009.01.051.Search in Google Scholar

[42] Gavaric N, Mozina S, Kladar N, Bozin B. Chemical profile, antioxidant and antibacterial activity of thyme and oregano essential oils, thymol and carvacrol and their possible synergism. J Essent Oil-Bear Plants. 2015;18:1013–21. 10.1080/0972060X.2014.971069.Search in Google Scholar

[43] Jouki M, Khazaei N. Effects of active batter coatings enriched by quince seed gum and carvacrol microcapsules on oil uptake and quality loss of nugget during frying. J Food Sci Technol. 2022; 59:1104–13. 10.1007/s13197-021-05114-4.Search in Google Scholar PubMed PubMed Central

Received: 2023-02-22
Revised: 2023-03-17
Accepted: 2023-03-31
Published Online: 2023-05-08

© 2023 the author(s), published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

Downloaded on 24.2.2024 from
Scroll to top button