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Licensed Unlicensed Requires Authentication Published by De Gruyter October 7, 2015

ABCG2/BCRP interaction with the sea grass Thalassia testudinum

Verónica Miguel, Jon A. Otero, Borja Barrera, Idania Rodeiro, Julio G. Prieto, Gracia Merino and Ana I. Álvarez

Abstract

Background: The aqueous ethanolic extract from leaves of the marine plant Thalassia testudinum has shown antioxidant, cytoprotective, and neuroprotective properties. The chemical composition of this extract, rich in polyphenols, could interfere with active transport of drugs out of the cell and circumvent the phenomenon of multidrug resistance (MDR). The extract can act as an MDR modulator through its interaction with efflux transporters. The ABCG2/BCRP has been shown to confer MDR acting in tumor cells.

Methods: To evaluate the interaction of ABCG2/BCRP with the extract, studies in cells overexpressing human BCRP transporter and its murine ortholog Bcrp1 were performed.

Results and conclusions: T. testudinum extract could be included as MDR modulator, as interaction with ABCG2/BCRP has been shown through flow cytometry and MTT assays. The cells overexpressing ABCG2/BCRP in the presence of the extract (25–150 μg/mL) decreased the survival rates of the anti-tumoral mitoxantrone. Our results support its inclusion as a possible MDR modulator against tumor cells that overexpress ABCG2/BCRP.


Corresponding author: Ana I. Álvarez, Departamento de Ciencias Biomédicas-Fisiología, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain; and Instituto de Desarrollo Ganadero y Sanidad Animal (INDEGSAL), Universidad de León, Campus de Vegazana s/n, 24071 León, Spain, Phone: +34 987 291 265; Fax: +34 987 291 261, E-mail:

Acknowledgments

The authors wish to express their gratitude to Dr. A.H. Schinkel (The Netherlands Cancer Institute, Amsterdam, The Netherlands), who provided MDCK-II cells and transduced subclones. We are grateful to James McCue for assistance in language editing.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2015-4-17
Accepted: 2015-9-1
Published Online: 2015-10-7
Published in Print: 2015-12-1

©2015 by De Gruyter

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