The experience of chronic pain is one of the commonest reasons for seeking medical attention, being a major issue in clinical practice. While pain is a universal experience, only a small proportion of people who felt pain develop pain syndromes. In addition, painkillers are associated with wide inter-individual variability in the analgesic response. This may be partly explained by the presence of single nucleotide polymorphisms in genes encoding molecular entities involved in pharmacodynamics and pharmacokinetics. However, uptake of this information has been slow due in large part to the lack of robust evidences demonstrating clinical utility. Furthermore, novel therapies, including targeting of epigenetic changes and gene therapy-based approaches are further broadening future options for the treatment of chronic pain. The aim of this article is to review the evidences behind pharmacogenetics (PGx) to individualize therapy (boosting the efficacy and minimizing potential toxicity) and genes implicated in pain medicine, in two parts: (i) genetic variability with pain sensitivity and analgesic response; and (ii) pharmacological concepts applied on PGx.
Pain is the most common presenting physical symptom in primary care, accounting for an enormous burden of patient suffering, quality of life, work disability, health care and societal costs. According to the International Association for the Study of Pain, pain is defined as “an unpleasant sensory and emotional experience associated with actual or potential tissue damage” . If untreated, chronic pain is very common and, approximately, one in three Americans and one in five Canadians and Europeans, are reported to suffer from this problem .
From a genetic point of view, chronic pain is a typical gene x environment interaction, where the inherited genetic predisposition can influence greatly the development of pain syndromes from various painful experiences . Different subtypes of pain can be described based on their neurophysiological basis and duration, including neuropathic, nociceptive, dysfunctional, acute and chronic. Treatment of pain-related suffering requires knowledge of how pain signals are initially interpreted and subsequently transmitted and perpetuated. In fact, the evolution and intensity of the painful experience shows great individual differences, even in response to the same stimulus in an experimental environment .
The enormous variability in individual response to the many disparate treatments for chronic pain is clinically important and needs to be better understood. The notion that “one size fits all” has been replaced by the idea of patient-tailored healthcare prevention and therapy. This is the base of personalized medicine. Within this paradigm, the research community has turned to examine genetic predictors of disease and treatment responses. Pain researchers performed genetic studies over the last decade that evaluated the association between genetic variability and pain sensitivity or analgesic response. Simultaneously, there is an increased recognition regarding the complexity of pain research, acknowledging the additional role of epigenetic, transcriptomic, proteomic and metabolomic factors in the development, experience and treatment of pain .
The relatively high heritability rate and the assumed mechanisms behind transition from acute to chronic pain suggest that genetic research might provide useful target molecules for future drug development, as well as, could be used as a tool to personalize the treatment of pain syndromes .
Gene-association studies searching for common genetic variants modulating pain vulnerability produced confounding results, probably because only few genetic regions were examined and its replication is very rarely found between the studies. In addition, most of these studies ignored the gene x environment interaction effect of stressful life events on vulnerability to pain that may contribute to transition to chronic pain state by triggering negative mood . Furthermore there are few meta-analyses in the field of pain genetics, and the only available considered the OPRM1 functional polymorphism 118A>G, but it did not confirm the relevance of this polymorphism in pain, only found weak evidences of less nausea and increased opioid intake in GG carriers , , .
A large meta-analysis of microarray studies for pain-induced gene expression changes in animal models resulted in the identification of genes commonly regulated by painful states. Immune system-related gene clusters showed the best association with induced pain suggesting a pronounced effect of inflammatory mechanisms underlying painful conditions. However, extrapolation of these results even between rat and mouse species failed . The differences in gene regulation observed in rat’s dorsal root ganglia (DRG) were almost completely absent in mice, despite the fact that the painful stimulus was the same. Therefore, cautious extrapolation of these results is required in human studies . Further research is required in order to assess the most pervasive factors in chronic pain development, and also to identify gene sets that shape the vulnerability profile toward those factors. With proper analgesic medication and concomitant personalized preventive therapy we can intervene the transition of the experienced pain into a chronic maladaptive state .
Genome-wide association studies (GWAS) have identified hundreds of genetic variants associated with complex human diseases and traits scanning markers across the complete sets of DNA. However, they rarely reported or even measured the experienced pain. This might be due to the lack of reliable and cheap pain level measurement tools, and, also, because of the fluctuations in the personal pain experience that makes very hard to detect true average pain intensity in cross-sectional studies .
Most variants identified so far confer relatively small increments in risk and explain only a small proportion of familial clustering, leading many to question how the remaining “missing” heritability could be explained. However, most of the studies compare the co-occurrence of the painful disease itself, and not the experienced pain states. Induced pain under laboratory circumstances can provide slightly better measurements of the heritability rate of pain-vulnerability, which is estimated among 22%–60% .
The rare exception of the multi-level GWAS study of chronic widespread pain resulted in the association of rheumatic pain with 5p12.2 chromosomal region . These results might facilitate to other research groups to consider experienced pain as a worthy target for GWAS analyses. In addition, novel therapies, including targeting of epigenetic changes and gene therapy-based approaches are further broadening future options for the treatment of chronic pain .
Epigenetic modifications, such as DNA methylation and histone modifications (e.g. acetylation and phosphorylation), are known to cause stable gene expression changes via chromatin remodeling. These mechanisms have a role not only in the determination of developmental cell fates, but also in the physiological and pathological processes in the nervous system. Alterations in DNA methylation, an enzymatic covalent modification of cytosine bases in the DNA, could serve as a “genomic” memory of pain in the adult cortex mediating the long-term consequences of painful experiences and embed them into the genome. DNA methylation is an epigenetic mechanism for long-term regulation of gene expression. Neuronal plasticity at the neuroanatomical, functional, morphological, physiological and molecular levels has been demonstrated throughout the neuroaxis in response to persistent pain, including in the adult prefrontal cortex. Importantly, there is emerging evidence that a variety of genes undergo epigenetic regulation via DNA methylation and histone modifications within peripheral and central nervous systems, thereby contributing to the alterations in both, pain sensitivity and pharmacological efficacy in neuropathic pain. It is discussed whether epigenetic mechanisms can serve as potential targets to treat neuropathic pain .
Living with unrelenting pain is maladaptive and is thought to be associated with physiological and psychological modifications, yet there is a lack of knowledge regarding brain elements involved in such conditions. The era of neuroimaging with functional magnetic resonance imaging (MRI) studies provide us a completely new aspect of pain genetic research. The capability for recording real-time activation of human brain in reaction to painful stimulus provides system-based biological phenotypes which are more able to assess the effect of genetic and neurobiological variables than simple syndrome phenotypes .
In fact, sustained high pain of chronic back pain (CBP) resulted in increased activity in the medial prefrontal cortex (including rostral anterior cingulate), strongly related to intensity of CBP, and this region is known to be involved in negative emotions, response to conflict and detection of unfavorable outcomes, especially in relation to the self. Interestingly, spontaneous CBP involves specific spatiotemporal neuronal mechanisms, distinct from those observed for acute experimental pain, implicating a salient role for emotional brain concerning the self .
Also, with MRI technology we can distinguish between different components of pain processing, such cognitive, affective or sensory elements and seems to be useful to entangle the previously confounding results about genetic predictor variants. These results suggest that different kinds of exposure to pain can cause transition to chronic pain states in different allele carriers, and also they would respond to psychological pain-management therapy differently .
In contrast to the wide variety of pain and painful syndromes, only a handful of pharmacological substances proved to be effective against pain. Benefit/risk ratio of all the analgesic drugs is unsatisfactory with either a limited efficacy or a high level of adverse effects and, sometimes, both.
Acetaminophen is one of the most popular and widely used drugs for the treatment of pain and fever. It occupies a unique position among analgesic drugs. Unlike non-steroidal anti-inflammatory drugs (NSAIDs), it is almost unanimously considered to have non-anti-inflammatory activity and does not produce gastrointestinal damage or untoward cardiorenal effects. Unlike opioids, it is almost ineffective in intense pain and has no depressant effect on respiration. A novel and original view of this molecule now proposes acetaminophen to be a pro-drug needed to be biotransformed to be analgesic  by (i) hepatic deacetylation of into para-aminophenol; (ii) conjugation by the cerebral fatty acid amide hydrolase enzyme into AM404  involving the activation of TRPV1 receptors , , followed by the inhibition of Cav3.2  and the modulation of CB1 receptors . This complex mechanism disinhibits the periaqueductal grey matter output neurons, which could promote activation of the descending serotonergic inhibitory pathways as shown both in animals , ,  and humans .
The COX inhibitors are used most commonly in noncancer pain, however, in patients with severe pain they are often proved to be ineffective, as well as, they present dangerous renal and gastrointestinal long-term side effects. In addition, they increase the risk of serious cardiovascular conditions, especially in patients with high risk for these conditions. The risks of these and other side effects increase in the elderly, when taken at higher doses, and with long-term use. As of April 2005, following recommendations by the US Food and Drug Administration issued a public health advisory stating that “NSAIDs should be administered at the lowest effective dose for the shortest duration consistent with individual patient treatment goals”. It is recommended that patients consult with their treating physician to evaluate the relative benefits and risks, especially of COX-2 inhibitors, in order to come up with the best treatment plan for their individual clinical situation , .
Opioids with their central mechanisms of action are controversial drugs in noncancer pain treatment. Apart from the most-discussed abuse potential, the tolerance and dependence developed after chronic administration are also limiting the usage of opioids against pain . Moreover, opioids suffer from invalidating adverse effects, which can alter the quality of life of patients and, in some rare cases, jeopardize the vital prognosis. They modulate nociception by stimulating μ opioid receptors (μOR), the major molecular gate for opioid analgesia . None of the pharmacological activities of morphine, either its analgesic or adverse effects, could be detected in mutant mice lacking μOR . This explains the difficulty to separate beneficial from adverse effects of μOR agonists. Recently, truncated μOR splice variants  have been proposed as targets to improve benefit/risk ratio of opiate analgesics . Several other strategies have been developed to try to reduce opioids adverse effects, such as using agonists of other opioid receptors ,  or peripheral μOR antagonists , . However, the former ones produce a limited analgesia  and the latter ones need to be co-prescribed with opioids and prevent constipation, but not morphine central adverse effects such as respiratory depression .
Recently, using a different strategy, it has been demonstrated that the TREK-1 K+ channel is a crucial contributor of morphine-induced analgesia in mice, while it is not involved in morphine-induced constipation, respiratory depression and/or dependence. These observations suggest that direct activation of the TREK-1 channel, acting downstream from the μOR, might have strong analgesic effects without opioids-like adverse effects , .
Empirically, it is well understood that large inter-individual variations exist with respect to the response to opioids . With conventional drug dosing, some patients will experience toxicity whereas other patients will not receive adequate analgesia at the same dose. Variations in analgesic efficacy can vary as much as two- to 10-fold or even 100-fold among members of the same family , .
Pharmacogenetics (PGx) refers to the way in which genetic differences between individuals influence patient drug responses and drug disposition , , . Generally, genes affecting outcome of treatment can be divided into two broad categories. On the one hand, genes affecting pharmacodynamics, based on variations in drug target receptors and downstream signal transduction (i.e. μ-opioid receptor, OPRM1; enzyme catecholamine methyltransferase, COMT, etc.) , . On the other hand genes affecting pharmacokinetics (PK) that affect drug metabolism and/or elimination (i.e. cytochrome P450 family of enzymes, enzymes responsible for glucuronidation, drug transporter proteins, COX enzymes, etc.) altering the relationship between drug dose and steady state serum drug concentrations .
Some candidate genes are implied either directly (opioid receptors) or indirectly into the opioid transduction pathways when signal is transmitted to a variety of effectors (e.g. adenylate cyclase or calcium and potassium ion channels named Kir3.2, KCNJ6).
Opioid receptors belong to the family of G-protein-coupled receptors (GPCRs). There are three types of classical opioid receptors: mu (μ), kappa (κ) and delta (δ). They share a high degree of homology and are structurally similar, containing an extracellular N-terminus domain, seven transmembrane domains and an intracellular C-terminus domain. Most variations are found in the N-terminal domain and extracellular loops. The extracellular loops determine ligand binding and are, therefore, particularly important. Splice variations of opioid receptor mRNA have been shown to produce receptor subtypes which may be of functional importance .
The μ-opioid receptor encoded by the opioid receptor μ1 (OPRM1) gene is the primary site of action for the most commonly used opioids. Therefore, it represents the first-line candidate for evaluating the role of polymorphisms in the clinical effects of morphine.
Studies in mice with targeted deletion of OPRM1 gene established that this receptor is essential for morphine analgesia, physical dependence and reward , . More than 100 single nucleotide polymorphisms (SNPs) localized in the C-terminal intracellular domain of the protein have been described, and could possibly participate in various transduction signaling pathways following agonist binding , .
The most common polymorphism C118A>G (rs1799971, A118G), leading to an asparagine to aspartate substitution (Asn40Asp), with an allelic frequency varying from 2% to 50% according to ethnic groups, has been extensively studied . This polymorphism is responsible for the loss of a putative N-linked glycosylation site in the N-terminal domain of the receptor and is associated with modified response to opiates. Indeed, the variant protein exhibits a three-times greater binding affinity for the endopeptide β-endorphin, whereas binding to substances such as morphine, methadone and naloxone was unaffected in vitro . Subjects carrying the variant G-allele were found to present reduced response to morphine treatment , , , ,  and reduced analgesic response to alfentanil and morphine-6-glucuronide , , , requiring higher doses of morphine for pain relief , . In addition, it is also associated with MOR expression, a variant associated with a decrease in both mRNA expression and translation into a functional protein . Also, lower mRNA expression in human brain tissue and in transfected cells was found in G-allele carriers .
Other SNPs from OPRM1 and the other classical opioid receptor genes, including OPRK1 and OPRD1, have been tested, for example, in the European Pharmacogenetic Opioid Study (EPOS). EPOS is the largest genetic association study of opioid response to date, with 2294 patients taking opioids for cancer-related pain. A total of 112 SNPs in 25 genes, including OPRM1, OPRK1 and OPRD1, were investigated for relationship to oral equivalent morphine dose requirements. However, no association was identified with any of the SNPs tested in both, development and validation analyses .
β-Arrestin 2 is an intracellular protein that is integral to μOR inactivation and internalization , , , , . On binding, opioid receptor agonists differentially trigger receptor phosphorylation and recruitment of β-arrestin. Knockout studies have shown that mice lacking β-arrestin 2 gene (ARRB2) exhibit prolonged analgesia from morphine treatment at lower doses . It is worth noting that prolonged analgesia in mice lacking ARRB2 may also be due to a combination of more complex effects transduced by multiple GPCRs in the knockout animal model . Polymorphisms in ARRB2 have been associated with overall response to morphine and opioid switching .
Other genes are implicated in the cellular transport of the molecules (such as ABCB1) or in their metabolism, which aims to convert lipophilic chemical compounds into more readily excreted hydrophilic products (mainly cytochrome isoforms CYP2D6, CYP3A4 and CYP2B6, and a glucuronosyltransferase implied in morphine metabolism, UGT2B7).
Different enzymes in phase 1 and/or phase 2 metabolisms are important for the metabolism of different opioids.
a) Phase 1 metabolism
a1) Cytochrome P450 2D6 (CYP2D26)
CYP2D26 is highly polymorphic and expression of different variants results in several phenotypes: poor metabolizers (PM) express two nonfunctional alleles (e.g. two of *4, *5, *6 or other alleles), intermediate metabolizers express at least one reduced functional allele (e.g. one of *9, *10, *41 or other alleles), extensive metabolizers (EM) express at least one functional allele, and ultrarapid metabolizers (UM) that present multiple copies of the functional allele. The prevalence of variant alleles exhibits considerable interethnic differences . The frequencies of these phenotypes in Caucasians are: PM, 5%–10%; intermediate metabolizers, 10%–15%; EM, 65%–80% and UM, 5%–10%. Well-characterized SNPs in CYP2D6 lead to the inability to convert codeine to morphine, thus making codeine ineffective as an analgesic for approximately 10% of the Caucasian population . In UM, codeine is convert to morphine and high concentrations of morphine can be observed  inducing major adverse effects. Tramadol is also metabolized through CYP2D6 and its analgesic effect may change according to the polymorphisms of this enzyme .
a2) Cytochrome P450 3A (CYP3A4) and 2B6 (CYP2B6)
The CYP3A4 enzyme is localized in the liver and small intestine and, thus, contributes to first-pass and systemic metabolism of opiates. CYP2B6 gene is a major isoform implied in methadone metabolism and clearance. However, although its activity is highly variable among individuals, no clear correlation between a genotype and a phenotype has yet been established .
b) Phase 2 metabolism
UGT2B7 insures the glucuronidation of morphine to morphine-6-glucuronide (M6G) and morphine-3-glucuronide (M3G) metabolites . M6G is approximately twice as potent as morphine in animal models and humans . Even though, M3G has little affinity for opioid receptors, but may contribute to excitatory effects of morphine . One frequent SNP, UGT2B7*2, has been studied (rs7439366, His268Tyr, 802C>T) that seems to be associated with a decreased activity .
P-glycoprotein 1 also known as multidrug resistance protein 1 or ATP-binding cassette sub-family B member 1 (ABCB1) is an important protein of the cell membrane involved in multidrug resistance. It is responsible for decreased drug accumulation in multidrug-resistant cells and also functions as a transporter in the blood-brain barrier. Mutations of the ABCB1 gene (SNP 1236C>T) have been associated with higher methadone doses (>150 mg/day) in methadone-maintained heroin addicts .
There has been noted to be an association between the ABCB1 and the OPRM1 gene polymorphisms related to morphine pain relief; combining evaluation of the two genes allowed detection of three response groups, resulting in a sensitivity close to 100% and specificity of more than 70% in predicting morphine relief . Also an association between COMT and OPRM1 gene polymorphisms has been found in other studies , . Carriers of both COMT Met/Met in Val158Met and OPRM1 A/A in A118G polymorphisms required less morphine than other subjects, however, differences were not significant and further studies should evaluate this association .
There may be differences between male and female patients at this gene as well; men with the TT allele had higher beta-endorphin levels than men with the more common CC allele, while the opposite was true in women . In addition, women with the TT allele presented a higher risk of postoperative pain 3 months after surgery . Similarly, female G-allele carriers of OPRM1 A118G SNP presented a slower recovery rate than male G-allele carriers after the disc herniation and increased pain intensity , , .
The involvement of catecholamines in pain modulation is known from both clinical and experimental studies . COMT is a key modulator of dopaminergic and adrenergic neurotransmission, and, as a consequence, in the reward signaling response to opioids. The C472G>A SNP of COMT (rs4680, Val158Met) causes a valine to methionine substitution at codon 158 in the enzyme. The Met allele leads to an enzyme up to four-times less active than the Val allele .
b) Potassium channel, inwardly rectifying subfamily J, member 6
This gene encodes a member of the G protein-coupled inwardly rectifying potassium channel family of inward rectifier potassium channels. This type of potassium channel allows a greater flow of potassium into the cell than out of it. These proteins modulate many physiological processes, including circuit activity in neuronal cells, through G-protein coupled receptor stimulation and it has been shown to participate in the modulation of analgesic effects on postsynaptic transmission and miosis under opioid treatment .
c) Serotonin transporter
Preliminary work in pain research is now emerging, with published studies that examine genotypic influence serotonin transporter (5HTT)gene polymorphisms on opioid analgesia in healthy volunteers and clinical response to anti-depressants , , .
In general, PGx studies thus far in pain management have failed to yield evidence of improved clinical outcomes associated with knowledge of patient genotypes when prescribing pain medications. Genetic factors are thought to be responsible for approximately 12%–60% of response variance in opioid treatment, as evaluated in twin studies . Many genes have been studied in order to identify PGx markers in opioid treatment, including genes implied in opioids’ pharmacodynamics and PK. In this review, we focus on those genes, although numerous genes have been implied in nociception and inflammation, and can participate in the analgesic dose requirement.
On an individual level, there is a difference in the analgesic response to a given opioid. Various factors such as gender, age and genetic variation can affect the analgesic response. In fact, opioid analgesia can be predicted from activity of reward-responsive brain regions during pain as well as subjects trait-reward responsiveness ratings , . Thus, studies have shown promising results regarding PGx as a diagnostic tool for predicting the individual response to a given opioid in experimental settings; however, in the clinic, it is a more complicated task to accomplish .
Neuropathic pain is characterized by complicated combination of positive (e.g. hyperalgesia and allodynia) and negative (e.g. hypoesthesia and hypoalgesia) symptoms, and is refractory to conventional pharmacological agents, including morphine.
Recently, Finnerup et al.  proposed a revision of the NeuPSIG (Special Interest Group on Neuropathic Pain, from the International Association for the Study of Pain) recommendations for the pharmacotherapy of neuropathic pain as follows: first-line treatment for tricyclic antidepressants, serotonin-noradrenalin reuptake inhibitors, pregabalin and gabapentin; second line for lidocaine patches, capsaicin high concentration patches and tramadol; third line for strong opioids and botulinum toxin A . They also stated that topical agents and botulinum toxin A are recommended only for peripheral neuropathic pain.
In recent years, it has begun to be appreciated that the pathobiology of various neuropathic pain subtypes may differ . As an example, chemotherapy-induced peripheral neuropathy (CIPN) is a common secondary toxicity to neurotoxic anticancer drugs. The type of anticancer drug and the cumulative dose may impact in the incidence (possibly until 90% of patients for oxaliplatin), the symptoms and the severity/grade of neuropathy . Recent meta-analysis demonstrated that among 31 studies (4179 patients), 68% (57.7–78.4) of patients suffered of CIPN 1 month after chemotherapy, 60% (36.4–81.6) after 3 months and 30% (6.4–53.5) at 6 months or more . In the case of oxaliplatin, which is probably the most neurotoxic anticancer drug, neuropathy symptoms can last until several years after the end of the chemotherapy cycles (2 years in the study by André et al.  and 8 years in the study of Yothers et al. ). Neurotoxicity mechanisms of anticancer drugs are not fully understood, but they may result from interactions with DNA, mitochondria, ion channels, glutamate neurotransmission and/or kinases, at various levels such as DRG (sensory neurons, Schwann cells, satellite cells) and spinal cord (neurons, glial cells) (for review, see Carozzi et al. ). CIPN is thus relatively distinct from other forms of neuropathic pain, including pathophysiology and symptomatology , . After a systematic literature search identifying randomized controlled trials for the treatment of CIPN, it has been concluded on the poor efficacy of these drugs in CIPN. Genetic factors may be important in predisposing patients to this adverse effect.
Chemotherapy-induced peripheral neuropathy and drug-induced peripheral neuropathies (DIPNs) are encountered, including small-fiber involvement. The introduction of new diagnostic techniques, such as excitability studies, skin laser Doppler flowmetry, and PGx, holds promise for early detection and elucidation of underlying mechanisms. New approaches to improve functions and quality of life in CIPN patients are discussed. Apart from developing less neurotoxic anticancer therapies, there is still hope to identify chemoprotective agents, such as erythropoietin and substances involved in the endocannabinoid system, able to prevent or correct painful CIPNs .
Increased susceptibility to peripheral neurotoxicity after exposure to offending agents has been associated with polymorphisms in genes involved in the following pathways: chemotherapy-induced DNA adducts repair , immune function (cytotoxic T-lymphocyte-associated protein 4), also known as CD152 (cluster of differentiation 152), CTLA4 and compatible time-sharing system, reflexive coupling within Schwann cells (Gap Junction Protein, Epsilon 1, GJE1), drug binding (proteasome subunit beta 1, PSMB1) and neuron function (Transcription Factor, 4TCF4 and dynein cytoplasmic 1 intermediate chain 1, DYNC1I1) , apoptosis , mitochondrial dysfunction, inflammation  and oxidative stress scavengers such as glutathione S-transferase 1 (GST1) .
Chemotherapy-induced peripheral neuropathy has been associated with variations in genes encoding for drug transporters, detoxification enzymes, genes involved in DNA repair mechanisms and integrin B3 Leu33Pro polymorphism .
For instance, polymorphisms of the gene encoding ABCB1, have been suggested to partially explain the variability of taxane-induced DIPN . It should be noted that several other studies have been unable to identify relevant associations , . Similarly, genetic variants of proteins involved in the metabolism of xenobiotics, for example, cytochrome 3A5, have been linked to increased risk of DIPN in children receiving vincristine . A range of polymorphisms have also been identified with GWAS in association with oxaliplatin , paclitaxel, bortezomib, thalidomide and vincristine , .
While the use of pharmacogenetic techniques to identify genetic polymorphisms has enabled further identification of potential differences in susceptibility to neurotoxicity between individual patients, it remains a lack of consensus on the association between genetic variants and the risk of neurotoxicity. Further studies with standardized objective measures of neuropathy, the choice of the good primary outcome and larger patient numbers will be required to fully assess the involvement of genetic polymorphisms in the risk of neurotoxicity.
A recently published collaborative international study attempted to overcome those limitations, thoroughly investigating a series of SNPs in genes coding for neurologically relevant targets, such as the voltage-gated sodium channels (SCNA), in an adequately powered, prospective cohort of well-characterized patients. SCNAs are fundamental to facilitate the initiation and propagation of action potentials in neurons. These membrane proteins are encoded by >10 genes in mammals, and mutations in SCNAs are associated with diseases of both, the central and peripheral nervous system . The results of this study provided evidences to support a causal relationship between SCN4A-rs2302237 and SCN10A-rs1263292 polymorphisms and increased incidence and/or severity of oxaliplatin-induced peripheral neuropathy . Further SCNA SNPs, such as the SCN2A R19K polymorphism, have been previously investigated with negative results . These results illustrate the difficulty to choose potential genetic biomarkers. Mutations in genes encoding SCNAs have emerged as the most clinically relevant genes associated with several pathologies. This is the case for SCN10A regarding peripheral pain disorders. However, SCN4A has not been associated with pain syndromes .
A better understanding of the pathophysiology of CIPN will certainly lead to identify new molecular targets for the prevention of this particular neuropathy. For example, mechanistically, oxaliplatin promotes neuronal over-excitability by drastically lowering the expression of distinct potassium channels (TREK, TRAAK) and by increasing the expression of pro-excitatory channels such as the hyperpolarization-activated channels (HCNs). These findings are corroborated by the analysis of TREK-TRAAK null mice and the use of the specific HCN inhibitor ivabradine, which abolishes the oxaliplatin-induced cold hypersensibility , .
These results suggest that oxaliplatin exacerbates cold perception by modulating the transcription of distinct ionic conductance that together shape sensory neuron response to cold. The translational and clinical implication of these findings would be that ivabradine, a nonspecific HCN blocker, or TREK and TREK agonists, may represent tailored treatment for oxaliplatin-induced neuropathy. Ivabradine, which has been developed to treat stable angina pectoris, is able to selectively and strongly attenuate the cold sensitization effects of oxaliplatin in mice. Therefore, as a drug already used in the clinic, it could rapidly become a new potential preventive analgesic treatment in patients undergoing oxaliplatin chemotherapy . Moreover, unpublished data from A. Eschalier’s group suggest the involvement of epigenetics in the transcriptional changes observed after oxaliplatin administration in mice, which opens to new pharmacological prevention strategies opportunities in this pathology.
Ideally, PGx studies aim to aid in the selection and dosing of an optimal drug therapy for a specific patient. Choosing the optimal therapy should lead to maximize therapeutic benefit, improved patient adherence and reduction in adverse drug reactions. However, patients are very commonly prescribed several medications for multiple co-morbidities and genetics can only partially explain the variability in patient responses to analgesic drugs.
Regardless that, one of the major challenges facing researchers working in this field, is translating their discoveries into clinical practice. As an emerging field, PGx confronts new challenges such as ensuring its correct standardization and its correct translation into routine clinical practise. The need to standardize how PGx information in order to translate it into routine clinical practices, to ensure dissemination of knowledge and education of clinicians and patients, need different multidisciplinary consortiums as the European Society of Pharmacogenomics and Personalised Therapy (ESPT). Furthermore, concerted efforts and open and active cooperation with industry, are required in order to facilitate translation and commercialization, avoiding to stuck PGx biomarkers in the discovery phase. Additionally, the knowledge and acceptance of new approaches to determine drug targets by clinicians, regulators, patients, and the public will be important in determining future success.
The review herein is based on the lectures presented by the speakers of the 3rd ESPT Conference, Budapest 2015 (October 7–9).
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
1. Kroenke K, Shen J, Oxman TE, Williams JW, Jr, Dietrich AJ. Impact of pain on the outcomes of depression treatment: results from the RESPECT trial. Pain 2008;134:209–15. Search in Google Scholar
2. Hardt J, Jacobsen C, Goldberg J, Nickel R, Buchwald D. Prevalence of chronic pain in a representative sample in the United States. Pain Med 2008;9:803–12. Search in Google Scholar
3. Bonica JJ. The relation of injury to pain. Pain 1979;7:203–7. Search in Google Scholar
4. Kapur BM, Lala PK, Shaw JL. Pharmacogenetics of chronic pain management. Clin Biochem 2014;47:1169–87. Search in Google Scholar
5. Ablin JN, Buskila D. Personalized treatment of pain. Curr Rheumatol Rep 2013;15:298. Search in Google Scholar
6. WHO. Cancer pain relief: with a guide to opioid availability, Geneve: World Health Organization, 1996. Search in Google Scholar
7. Juhasz G, Hullam G, Eszlari N, Gonda X, Antal P, Anderson IM, et al. Brain galanin system genes interact with life stresses in depression-related phenotypes. Proc Natl Acad Sci USA 2014;111:E1666–73. Search in Google Scholar
8. Walter C, Lotsch J. Meta-analysis of the relevance of the OPRM1 118A>G genetic variant for pain treatment. Pain 2009;146:270–5. Search in Google Scholar
9. Hwang IC, Park JY, Myung SK, Ahn HY, Fukuda K, Liao Q. OPRM1 A118G gene variant and postoperative opioid requirement: a systematic review and meta-analysis. Anesthesiology 2014;121:825–34. Search in Google Scholar
10. Ren ZY, Xu XQ, Bao YP, He J, Shi L, Deng JH, et al. The impact of genetic variation on sensitivity to opioid analgesics in patients with postoperative pain: a systematic review and meta-analysis. Pain Phys 2015;18:131–52. Search in Google Scholar
11. Lee M, Tracey I. Neuro-genetics of persistent pain. Curr Opin Neurobiol 2013;23:127–32. Search in Google Scholar
12. Svetlik S, Hronova K, Bakhouche H, Matouskova O, Slanar O. Pharmacogenetics of chronic pain and its treatment. Mediators Inflamm 2013;2013:864319. Search in Google Scholar
13. Peters MJ, Broer L, Willemen HL, Eiriksdottir G, Hocking LJ, Holliday KL, et al. Genome-wide association study meta-analysis of chronic widespread pain: evidence for involvement of the 5p15.2 region. Ann Rheum Dis 2013;72:427–36. Search in Google Scholar
14. Manolio TA, Collins FS, Cox NJ, Goldstein DB, Hindorff LA, Hunter DJ, et al. Finding the missing heritability of complex diseases. Nature 2009;461:747–53. Search in Google Scholar
15. Pirmohamed M. Personalized pharmacogenomics: predicting efficacy and adverse drug reactions. Annu Rev Genomics Hum Genet 2014;15:349–70. Search in Google Scholar
16. Ueda H, Uchida H. Epigenetic modification in neuropathic pain. Curr Pharm Des 2015;21:849–67. Search in Google Scholar
17. Tracey I, Mantyh PW. The cerebral signature for pain perception and its modulation. Neuron 2007;55:377–91. Search in Google Scholar
18. Baliki MN, Chialvo DR, Geha PY, Levy RM, Harden RN, Parrish TB, et al. Chronic pain and the emotional brain: specific brain activity associated with spontaneous fluctuations of intensity of chronic back pain. J Neurosci 2006;26:12165–73. Search in Google Scholar
19. Mallet C, Daulhac L, Bonnefont J, Ledent C, Etienne M, Chapuy E, et al. Endocannabinoid and serotonergic systems are needed for acetaminophen-induced analgesia. Pain 2008;139:190–200. Search in Google Scholar
20. Dalmann R, Daulhac L, Antri M, Eschalier A, Mallet C. Supra-spinal FAAH is required for the analgesic action of paracetamol in an inflammatory context. Neuropharmacology 2015;91:63–70. Search in Google Scholar
21. Kerckhove N, Mallet C, Francois A, Boudes M, Chemin J, Voets T, et al. Ca(v)3.2 calcium channels: the key protagonist in the supraspinal effect of paracetamol. Pain 2014;155:764–72. Search in Google Scholar
22. Barriere DA, Mallet C, Blomgren A, Simonsen C, Daulhac L, Libert F, et al. Fatty acid amide hydrolase-dependent generation of antinociceptive drug metabolites acting on TRPV1 in the brain. PLoS One 2013;8:e70690. Search in Google Scholar
23. Bonnefont J, Alloui A, Chapuy E, Clottes E, Eschalier A. Orally administered paracetamol does not act locally in the rat formalin test: evidence for a supraspinal, serotonin-dependent antinociceptive mechanism. Anesthesiology 2003;99:976–81. Search in Google Scholar
24. Pini LA, Sandrini M, Vitale G. The antinociceptive action of paracetamol is associated with changes in the serotonergic system in the rat brain. Eur J Pharmacol 1996;308:31–40. Search in Google Scholar
25. Tjolsen A, Lund A, Hole K. Antinociceptive effect of paracetamol in rats is partly dependent on spinal serotonergic systems. Eur J Pharmacol 1991;193:193–201. Search in Google Scholar
26. Pickering G, Esteve V, Loriot MA, Eschalier A, Dubray C. Acetaminophen reinforces descending inhibitory pain pathways. Clin Pharmacol Ther 2008;84:47–51. Search in Google Scholar
27. Enthoven WT, Roelofs PD, Deyo RA, van Tulder MW, Koes BW. Non-steroidal anti-inflammatory drugs for chronic low back pain. Cochrane Database Syst Rev 2016;2:CD012087. Search in Google Scholar
28. Fine M. Quantifying the impact of NSAID-associated adverse events. Am J Manag Care 2013;19:s267–72. Search in Google Scholar
29. Furlan AD, Reardon R, Weppler C, National Opioid Use Guideline G. Opioids for chronic noncancer pain: a new Canadian practice guideline. Can Med Assoc J 2010;182:923–30. Search in Google Scholar
30. Kieffer BL, Gaveriaux-Ruff C. Exploring the opioid system by gene knockout. Progr Neurobiol 2002;66:285–306. Search in Google Scholar
31. Matthes HW, Maldonado R, Simonin F, Valverde O, Slowe S, Kitchen I, et al. Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the mu-opioid-receptor gene. Nature 1996;383:819–23. Search in Google Scholar
32. Majumdar S, Grinnell S, Le Rouzic V, Burgman M, Polikar L, Ansonoff M, et al. Truncated G protein-coupled mu opioid receptor MOR-1 splice variants are targets for highly potent opioid analgesics lacking side effects. Proc Natl Acad Sci USA 2011;108:19778–83. Search in Google Scholar
33. Majumdar S, Subrath J, Le Rouzic V, Polikar L, Burgman M, Nagakura K, et al. Synthesis and evaluation of aryl-naloxamide opiate analgesics targeting truncated exon 11-associated mu opioid receptor (MOR-1) splice variants. J Med Chem 2012;55:6352–62. Search in Google Scholar
34. Vanderah TW. Delta and kappa opioid receptors as suitable drug targets for pain. Clin J Pain 2010;26:S10–15. Search in Google Scholar
35. Pradhan AA, Befort K, Nozaki C, Gaveriaux-Ruff C, Kieffer BL. The delta opioid receptor: an evolving target for the treatment of brain disorders. Trends Pharmacol Sci 2011;32:581–90. Search in Google Scholar
36. Stephenson J. Methylnaltrexone reverses opioid-induced constipation. Lancet Oncol 2002;3:202. Search in Google Scholar
37. Webster L, Jansen JP, Peppin J, Lasko B, Irving G, Morlion B, et al. Alvimopan, a peripherally acting mu-opioid receptor (PAM-OR) antagonist for the treatment of opioid-induced bowel dysfunction: results from a randomized, double-blind, placebo-controlled, dose-finding study in subjects taking opioids for chronic non-cancer pain. Pain 2008;137:428–40. Search in Google Scholar
38. Riviere PJ. Peripheral kappa-opioid agonists for visceral pain. Br J Pharmacol 2004;141:1331–4. Search in Google Scholar
39. Bates JJ, Foss JF, Murphy DB. Are peripheral opioid antagonists the solution to opioid side effects? Anesth Analg 2004;98:116–22, table of contents. Search in Google Scholar
40. Devilliers M, Busserolles J, Lolignier S, Deval E, Pereira V, Alloui A, et al. Activation of TREK-1 by morphine results in analgesia without adverse side effects. Nat Commun 2013;4:2941. Search in Google Scholar
41. Rodrigues N, Bennis K, Vivier D, Pereira V, Chatelain FC, Chapuy E, et al. Synthesis and structure–activity relationship study of substituted caffeate esters as antinociceptive agents modulating the TREK-1 channel. Eur J Med Chem 2014;75: 391–402. Search in Google Scholar
42. Stamer UM, Zhang L, Stuber F. Personalized therapy in pain management: where do we stand? Pharmacogenomics 2010;11:843–64. Search in Google Scholar
43. Lalovic B, Kharasch E, Hoffer C, Risler L, Liu-Chen LY, Shen DD. Pharmacokinetics and pharmacodynamics of oral oxycodone in healthy human subjects: role of circulating active metabolites. Clin Pharmacol Ther 2006;79:461–79. Search in Google Scholar
44. Stamer UM, Stuber F. Genetic factors in pain and its treatment. Curr Opin Anaesthesiol 2007;20:478–84. Search in Google Scholar
45. Pirmohamed M. Genetics and the potential for predictive tests in adverse drug reactions. Chem Immunol Allergy 2012;97:18–31. Search in Google Scholar
46. Klepstad P, Rakvag TT, Kaasa S, Holthe M, Dale O, Borchgrevink PC, et al. The 118 A > G polymorphism in the human mu-opioid receptor gene may increase morphine requirements in patients with pain caused by malignant disease. Acta Anaesthesiol Scand 2004;48:1232–9. Search in Google Scholar
47. Kadiev E, Patel V, Rad P, Thankachan L, Tram A, Weinlein M, et al. Role of pharmacogenetics in variable response to drugs: focus on opioids. Expert Opin Drug Metab Toxicol 2008;4:77–91. Search in Google Scholar
48. Kirchheiner J, Schmidt H, Tzvetkov M, Keulen JT, Lotsch J, Roots I, et al. Pharmacokinetics of codeine and its metabolite morphine in ultra-rapid metabolizers due to CYP2D6 duplication. Pharmacogenomics J 2007;7:257–65. Search in Google Scholar
49. Dietis N, Rowbotham DJ, Lambert DG. Opioid receptor subtypes: fact or artifact? Br J Anaesth 2011;107:8–18. Search in Google Scholar
50. Lancaster TM, Linden DE, Heerey EA. COMT val158met predicts reward responsiveness in humans. Genes Brain Behav 2012;11:986–92. Search in Google Scholar
51. Ravindranathan A, Joslyn G, Robertson M, Schuckit MA, Whistler JL, White RL. Functional characterization of human variants of the mu-opioid receptor gene. Proc Natl Acad Sci USA 2009;106:10811–6. Search in Google Scholar
52. Shabalina SA, Zaykin DV, Gris P, Ogurtsov AY, Gauthier J, Shibata K, et al. Expansion of the human mu-opioid receptor gene architecture: novel functional variants. Hum Mol Genet 2009;18:1037–51. Search in Google Scholar
53. Brunton L, Chabner B, Knollman B. Goodman and Gilman’s the pharmacological basis of therapeutics, 12th ed. New York: McGraw-Hill Education, 2011. Search in Google Scholar
54. Kreek MJ, Bart G, Lilly C, LaForge KS, Nielsen DA. Pharmacogenetics and human molecular genetics of opiate and cocaine addictions and their treatments. Pharmacol Rev 2005;57:1–26. Search in Google Scholar
55. Chou WY, Wang CH, Liu PH, Liu CC, Tseng CC, Jawan B. Human opioid receptor A118G polymorphism affects intravenous patient-controlled analgesia morphine consumption after total abdominal hysterectomy. Anesthesiology 2006;105:334–7. Search in Google Scholar
56. Chou WY, Yang LC, Lu HF, Ko JY, Wang CH, Lin SH, et al. Association of mu-opioid receptor gene polymorphism (A118G) with variations in morphine consumption for analgesia after total knee arthroplasty. Acta Anaesthesiol Scand 2006;50:787–92. Search in Google Scholar
57. Zhang Y, Wang D, Johnson AD, Papp AC, Sadee W. Allelic expression imbalance of human mu opioid receptor (OPRM1) caused by variant A118G. J Biol Chem 2005;280:32618–24. Search in Google Scholar
58. Romberg RR, Olofsen E, Bijl H, Taschner PE, Teppema LJ, Sarton EY, et al. Polymorphism of mu-opioid receptor gene (OPRM1:c.118A>G) does not protect against opioid-induced respiratory depression despite reduced analgesic response. Anesthesiology 2005;102:522–30. Search in Google Scholar
59. Skarke C, Darimont J, Schmidt H, Geisslinger G, Lotsch J. Analgesic effects of morphine and morphine-6-glucuronide in a transcutaneous electrical pain model in healthy volunteers. Clin Pharmacol Ther 2003;73:107–21. Search in Google Scholar
60. Klepstad P, Fladvad T, Skorpen F, Bjordal K, Caraceni A, Dale O, et al. Influence from genetic variability on opioid use for cancer pain: a European genetic association study of 2294 cancer pain patients. Pain 2011;152:1139–45. Search in Google Scholar
61. Bond C, LaForge KS, Tian M, Melia D, Zhang S, Borg L, et al. Single-nucleotide polymorphism in the human mu opioid receptor gene alters beta-endorphin binding and activity: possible implications for opiate addiction. Proc Natl Acad Sci USA 1998;95:9608–13. Search in Google Scholar
62. Ross JR, Rutter D, Welsh K, Joel SP, Goller K, Wells AU, et al. Clinical response to morphine in cancer patients and genetic variation in candidate genes. Pharmacogenomics J 2005;5:324–36. Search in Google Scholar
63. Steele AD, Szabo I, Bednar F, Rogers TJ. Interactions between opioid and chemokine receptors: heterologous desensitization. Cytokine Growth Factor Rev 2002;13:209–22. Search in Google Scholar
64. Zaki PA, Keith DE, Jr, Brine GA, Carroll FI, Evans CJ. Ligand-induced changes in surface mu-opioid receptor number: relationship to G protein activation? J Pharmacol Exp Ther 2000;292:1127–34. Search in Google Scholar
65. Zhang Y, Xiong W, Lin X, Ma X, Yu LC. Receptor trafficking induced by mu-opioid-receptor phosphorylation. Neurosci Biobehav Rev 2009;33:1192–7. Search in Google Scholar
66. Bohn LM, Lefkowitz RJ, Gainetdinov RR, Peppel K, Caron MG, Lin FT. Enhanced morphine analgesia in mice lacking beta-arrestin 2. Science 1999;286:2495–8. Search in Google Scholar
67. Branford R, Droney J, Ross JR. Opioid genetics: the key to personalized pain control? Clin Genet 2012;82:301–10. Search in Google Scholar
68. Darbari DS, van Schaik RH, Capparelli EV, Rana S, McCarter R, van den Anker J. UGT2B7 promoter variant -840G>A contributes to the variability in hepatic clearance of morphine in patients with sickle cell disease. Am J Hematol 2008;83:200–2. Search in Google Scholar
69. Fricke-Galindo I, Cespedes-Garro C, Rodrigues-Soares F, Naranjo ME, Delgado A, de Andres F, et al. Interethnic variation of CYP2C19 alleles, ‘predicted’ phenotypes and ‘measured’ metabolic phenotypes across world populations. Pharmacogenomics J 2016;16:113–23. Search in Google Scholar
70. Desmeules J, Gascon MP, Dayer P, Magistris M. Impact of environmental and genetic factors on codeine analgesia. Eur J Clin Pharmacol 1991;41:23–6. Search in Google Scholar
71. Stamer UM, Lehnen K, Hothker F, Bayerer B, Wolf S, Hoeft A, et al. Impact of CYP2D6 genotype on postoperative tramadol analgesia. Pain 2003;105:231–8. Search in Google Scholar
72. Linares OA, Fudin J, Schiesser WE, Daly Linares AL, Boston RC. CYP2D6 phenotype-specific codeine population pharmacokinetics. J Pain Palliat Care Pharmacother 2015;29:4–15. Search in Google Scholar
73. Campa D, Gioia A, Tomei A, Poli P, Barale R. Association of ABCB1/MDR1 and OPRM1 gene polymorphisms with morphine pain relief. Clin Pharmacol Ther 2008;83:559–66. Search in Google Scholar
74. de Wildt SN, Kearns GL, Leeder JS, van den Anker JN. Glucuronidation in humans. Pharmacogenetic and developmental aspects. Clin Pharmacokinet 1999;36:439–52. Search in Google Scholar
75. Trescot AM, Datta S, Lee M, Hansen H. Opioid pharmacology. Pain Phys 2008;11:S133–53. Search in Google Scholar
76. Bhasker CR, McKinnon W, Stone A, Lo AC, Kubota T, Ishizaki T, et al. Genetic polymorphism of UDP-glucuronosyltransferase 2B7 (UGT2B7) at amino acid 268: ethnic diversity of alleles and potential clinical significance. Pharmacogenetics 2000;10: 679–85. Search in Google Scholar
77. Lotsch J, Pruss H, Veh RW, Doehring A. A KCNJ6 (Kir3.2, GIRK2) gene polymorphism modulates opioid effects on analgesia and addiction but not on pupil size. Pharmacogenet Genomics 2010;20:291–7. Search in Google Scholar
78. Sia AT, Sng BL, Lim EC, Law H, Tan EC. The influence of ATP-binding cassette sub-family B member-1 (ABCB1) genetic polymorphisms on acute and chronic pain after intrathecal morphine for caesarean section: a prospective cohort study. Int J Obstet Anesth 2010;19:254–60. Search in Google Scholar
79. De Gregori M, Garbin G, De Gregori S, Minella CE, Bugada D, Lisa A, et al. Genetic variability at COMT but not at OPRM1 and UGT2B7 loci modulates morphine analgesic response in acute postoperative pain. Eur J Clin Pharmacol 2013;69:1651–8. Search in Google Scholar
80. Matic M, Simons SH, van Lingen RA, van Rosmalen J, Elens L, de Wildt SN, et al. Rescue morphine in mechanically ventilated newborns associated with combined OPRM1 and COMT genotype. Pharmacogenomics 2014;15:1287–95. Search in Google Scholar
81. Levran O, O’Hara K, Peles E, Li D, Barral S, Ray B, et al. ABCB1 (MDR1) genetic variants are associated with methadone doses required for effective treatment of heroin dependence. Hum Mol Genet 2008;17:2219–27. Search in Google Scholar
82. Rhodin A, Gronbladh A, Ginya H, Nilsson KW, Rosenblad A, Zhou Q, et al. Combined analysis of circulating beta-endorphin with gene polymorphisms in OPRM1, CACNAD2 and ABCB1 reveals correlation with pain, opioid sensitivity and opioid-related side effects. Mol Brain 2013;6:8. Search in Google Scholar
83. Fillingim RB, Kaplan L, Staud R, Ness TJ, Glover TL, Campbell CM, et al. The A118G single nucleotide polymorphism of the mu-opioid receptor gene (OPRM1) is associated with pressure pain sensitivity in humans. J Pain 2005;6:159–67. Search in Google Scholar
84. Hasvik E, Iordanova Schistad E, Grovle L, Julsrud Haugen A, Roe C, Gjerstad J. Subjective health complaints in patients with lumbar radicular pain and disc herniation are associated with a sex-OPRM1 A118G polymorphism interaction: a prospective 1-year observational study. BMC Musculoskelet Disord 2014;15:161. Search in Google Scholar
85. Olsen MB, Jacobsen LM, Schistad EI, Pedersen LM, Rygh LJ, Roe C, et al. Pain intensity the first year after lumbar disc herniation is associated with the A118G polymorphism in the opioid receptor mu 1 gene: evidence of a sex and genotype interaction. J Neurosci 2012;32:9831–4. Search in Google Scholar
86. Niemi G, Breivik H. Epinephrine markedly improves thoracic epidural analgesia produced by a small-dose infusion of ropivacaine, fentanyl, and epinephrine after major thoracic or abdominal surgery: a randomized, double-blinded crossover study with and without epinephrine. Anesth Analg 2002;94:1598–605, table of contents. Search in Google Scholar
87. Jimenez N, Galinkin JL. Personalizing pediatric pain medicine: using population-specific pharmacogenetics, genomics, and other -omics approaches to predict response. Anesth Analg 2015;121:183–7. Search in Google Scholar
88. Jensen KB, Lonsdorf TB, Schalling M, Kosek E, Ingvar M. Increased sensitivity to thermal pain following a single opiate dose is influenced by the COMT val(158)met polymorphism. PLoS One 2009;4:e6016. Search in Google Scholar
89. Kosek E, Jensen KB, Lonsdorf TB, Schalling M, Ingvar M. Genetic variation in the serotonin transporter gene (5-HTTLPR, rs25531) influences the analgesic response to the short acting opioid Remifentanil in humans. Mol Pain 2009;5:37. Search in Google Scholar
90. Angst MS, Phillips NG, Drover DR, Tingle M, Galinkin JL, Christians U, et al. Opioid pharmacogenomics using a twin study paradigm: methods and procedures for determining familial aggregation and heritability. Twin Res Hum Genet 2010;13:412–25. Search in Google Scholar
91. Wanigasekera V, Lee MC, Rogers R, Kong Y, Leknes S, Andersson J, et al. Baseline reward circuitry activity and trait reward responsiveness predict expression of opioid analgesia in healthy subjects. Proc Natl Acad Sci USA 2012;109:17705–10. Search in Google Scholar
92. Uher R, Huezo-Diaz P, Perroud N, Smith R, Rietschel M, Mors O, et al. Genetic predictors of response to antidepressants in the GENDEP project. Pharmacogenomics J 2009;9:225–33. Search in Google Scholar
93. Kharasch ED, Hoffer C, Whittington D, Sheffels P. Role of hepatic and intestinal cytochrome P450 3A and 2B6 in the metabolism, disposition, and miotic effects of methadone. Clin Pharmacol Ther 2004;76:250–69. Search in Google Scholar
94. Finnerup NB, Attal N, Haroutounian S, McNicol E, Baron R, Dworkin RH, et al. Pharmacotherapy for neuropathic pain in adults: a systematic review and meta-analysis. Lancet Neurol 2015;14:162–73. Search in Google Scholar
95. Jensen TS, Baron R, Haanpaa M, Kalso E, Loeser JD, Rice AS, et al. A new definition of neuropathic pain. Pain 2011;152:2204–5. Search in Google Scholar
96. Balayssac D, Ferrier J, Descoeur J, Ling B, Pezet D, Eschalier A, et al. Chemotherapy-induced peripheral neuropathies: from clinical relevance to preclinical evidence. Expert Opin Drug Saf 2011;10:407–17. Search in Google Scholar
97. Seretny M, Currie GL, Sena ES, Ramnarine S, Grant R, MacLeod MR, et al. Incidence, prevalence, and predictors of chemotherapy-induced peripheral neuropathy: a systematic review and meta-analysis. Pain 2014;155:2461–70. Search in Google Scholar
98. André T, Boni C, Navarro M, Tabernero J, Hickish T, Topham C, et al. Improved overall survival with oxaliplatin, fluorouracil, and leucovorin as adjuvant treatment in stage II or III colon cancer in the MOSAIC trial. J Clin Oncol 2009;27:3109–16. Search in Google Scholar
99. Yothers G, O’Connell MJ, Allegra CJ, Kuebler JP, Colangelo LH, Petrelli NJ, et al. Oxaliplatin as adjuvant therapy for colon cancer: updated results of NSABP C-07 trial, including survival and subset analyses. J Clin Oncol 2011;29:3768–74. Search in Google Scholar
100. Carozzi VA, Canta A, Chiorazzi A. Chemotherapy-induced peripheral neuropathy: what do we know about mechanisms? Neurosci Lett 2015;596:90–107. Search in Google Scholar
101. Wolf SL, Barton DL, Qin R, Wos EJ, Sloan JA, Liu H, et al. The relationship between numbness, tingling, and shooting/burning pain in patients with chemotherapy-induced peripheral neuropathy (CIPN) as measured by the EORTC QLQ-CIPN20 instrument, N06CA. Support Care Cancer 2012;20:625–32. Search in Google Scholar
102. Hershman DL, Lacchetti C, Dworkin RH, Lavoie Smith EM, Bleeker J, Cavaletti G, et al. Prevention and management of chemotherapy-induced peripheral neuropathy in survivors of adult cancers: American Society of Clinical Oncology clinical practice guideline. J Clin Oncol 2014;32:1941–67. Search in Google Scholar
103. Alvarado S, Tajerian M, Suderman M, Machnes Z, Pierfelice S, Millecamps M, et al. An epigenetic hypothesis for the genomic memory of pain. Front Cell Neurosci 2015;9:88. Search in Google Scholar
104. Egbelakin A, Ferguson MJ, MacGill EA, Lehmann AS, Topletz AR, Quinney SK, et al. Increased risk of vincristine neurotoxicity associated with low CYP3A5 expression genotype in children with acute lymphoblastic leukemia. Pediatr Blood Cancer 2011;56:361–7. Search in Google Scholar
105. Dzagnidze A, Katsarava Z, Makhalova J, Liedert B, Yoon MS, Kaube H, et al. Repair capacity for platinum-DNA adducts determines the severity of cisplatin-induced peripheral neuropathy. J Neurosci 2007;27:9451–7. Search in Google Scholar
106. Favis R, Sun Y, van de Velde H, Broderick E, Levey L, Meyers M, et al. Genetic variation associated with bortezomib-induced peripheral neuropathy. Pharmacogenet Genomics 2011;21:121–9. Search in Google Scholar
107. Broyl A, Corthals SL, Jongen JL, van der Holt B, Kuiper R, de Knegt Y, et al. Mechanisms of peripheral neuropathy associated with bortezomib and vincristine in patients with newly diagnosed multiple myeloma: a prospective analysis of data from the HOVON-65/GMMG-HD4 trial. Lancet Oncol 2010;11:1057–65. Search in Google Scholar
108. Johnson DC, Corthals SL, Walker BA, Ross FM, Gregory WM, Dickens NJ, et al. Genetic factors underlying the risk of thalidomide-related neuropathy in patients with multiple myeloma. J Clin Oncol 2011;29:797–804. Search in Google Scholar
109. McWhinney SR, Goldberg RM, McLeod HL. Platinum neurotoxicity pharmacogenetics. Mol Cancer Ther 2009;8:10–16. Search in Google Scholar
110. Diezi M, Buclin T, Kuntzer T. Toxic and drug-induced peripheral neuropathies: updates on causes, mechanisms and management. Curr Opin Neurol 2013;26:481–8. Search in Google Scholar
111. Marsh S, Paul J, King CR, Gifford G, McLeod HL, Brown R. Pharmacogenetic assessment of toxicity and outcome after platinum plus taxane chemotherapy in ovarian cancer: the Scottish Randomised Trial in Ovarian Cancer. J Clin Oncol 2007;25:4528–35. Search in Google Scholar
112. Bergmann TK, Gréen H, Brasch-Andersen C, Mirza MR, Herrstedt J, Hølund B, et al. Retrospective study of the impact of pharmacogenetic variants on paclitaxel toxicity and survival in patients with ovarian cancer. Eur J Clin Pharmacol 2011;67:693–700. Search in Google Scholar
113. Sissung TM, Baum CE, Deeken J, Price DK, Aragon-Ching J, Steinberg SM, et al. ABCB1 genetic variation influences the toxicity and clinical outcome of patients with androgen-independent prostate cancer treated with docetaxel. Clin Cancer Res 2008;14:4543–9. Search in Google Scholar
114. Won HH, Lee J, Park JO, Park YS, Lim HY, Kang WK, et al. Polymorphic markers associated with severe oxaliplatin-induced, chronic peripheral neuropathy in colon cancer patients. Cancer 2012;118:2828–36. Search in Google Scholar
115. Catterall WA. From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels. Neuron 2000;26:13–25. Search in Google Scholar
116. Argyriou AA, Cavaletti G, Antonacopoulou A, Genazzani AA, Briani C, Bruna J, et al. Voltage-gated sodium channel polymorphisms play a pivotal role in the development of oxaliplatin-induced peripheral neurotoxicity: results from a prospective multicenter study. Cancer 2013;119:3570–7. Search in Google Scholar
117. Cavaletti G, Alberti P, Marmiroli P. Chemotherapy-induced peripheral neurotoxicity in the era of pharmacogenomics. Lancet Oncol 2011;12:1151–61. Search in Google Scholar
118. Brunklaus A, Ellis R, Reavey E, Semsarian C, Zuberi SM. Genotype phenotype associations across the voltage-gated sodium channel family. J Med Genet 2014;51:650–8. Search in Google Scholar
119. Descoeur J, Pereira V, Pizzoccaro A, Francois A, Ling B, Maffre V, et al. Oxaliplatin-induced cold hypersensitivity is due to remodelling of ion channel expression in nociceptors. EMBO Mol Med 2011;3:266–78. Search in Google Scholar
120. Pereira V, Busserolles J, Christin M, Devilliers M, Poupon L, Legha W, et al. Role of the TREK2 potassium channel in cold and warm thermosensation and in pain perception. Pain 2014;155:2534–44. Search in Google Scholar
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