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Licensed Unlicensed Requires Authentication Published by De Gruyter November 25, 2017

In vitro modulation of the cytochrome P450 and ABCB1/P-glycoprotein activities of the aqueous extract of Allophylus cominia (L) Sw. leaves

  • Carlos L. Pérez , Maria T. Donato , Ivones Hernández , Miriam T. Paz Lopes , Evangelina Marrero , Jose A. Herrera , Maria J. Gómez-Lechón and Idania Rodeiro EMAIL logo



The aqueous extract of the Allophylus cominia (L) Sw (Sapindaceae) leaves has shown anti-diabetic, anti-obesity and anti-inflammatory properties. In the Caribbean region, it is typically used for the treatment of type-2 diabetes.


Considering the herb–drug interaction, the aim of this study was to evaluate the potential effects of the A. cominia extract on the cytochrome P450 (CYP) (rat hepatocyte model) and P-glycoprotein (P-gp) (4T1 cell line) systems.


The extract did not decrease the cell viability after being assayed by the MTT test at up to 1500 μg/mL for 72 h. The exposure of the cultured rat hepatocytes to the product (up to 250 μg/mL) for 48 h increased the activities of CYP-1A2, 2C9, and 2E1 by 1.46-, 1.60-, and 1.51-fold, respectively, compared with the controls. The activities of CYP-2B6, 2D6, and 3A4 were not significantly altered, whereas the activity of P-gp decreased by 2- and 4-fold. In addition, the extracts at 100 and 200 μg/mL significantly increased doxorubicin cytotoxicity in these cells 24 h after treatment.


The findings indicate that the A. cominia extract modulates the CYP and P-gp systems increasing sensitivity to doxorubicin. Further studies are necessary to evaluate the potential herb–drug interaction or chemosensitive properties.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: C.L. Pérez holds a Young Researchers fellowship from the University of Valencia, Funder Id: 10.13039/501100003508, Spain. Funds from IIS La Fe Hospital, Valencia, Spain and the Visiting Research Program (PVE-Research Project, no. 400768/2014-3), Funder Id: 10.13039/501100003593, CNPq, Brasil.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


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Received: 2017-6-2
Accepted: 2017-9-25
Published Online: 2017-11-25
Published in Print: 2017-12-20

©2017 Walter de Gruyter GmbH, Berlin/Boston

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