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BY-NC-ND 4.0 license Open Access Published by De Gruyter Open Access September 25, 2018

Fabrication and Bioapplications of Magnetically Modified Chitosan-based Electrospun Nanofibers

  • Ivo Safarik EMAIL logo , Kristyna Pospiskova , Eva Baldikova , Ioanna Savva , Ladislau Vekas , Oana Marinica , Eugenia Tanasa and Theodora Krasia-Christoforou
From the journal Electrospinning


The fabrication of magnetically modified electrospun nanocomposite fibers based on a naturally-derived biocompatible and biodegradable polysaccharide chitosan (CS) and the hydrophilic and biocompatible poly(vinylpyrrolidone) (PVP) is reported herein. The anchoring of magnetic nanoparticles (MNPs) onto the surfaces of the electrospun PVP/CS fibers was carried out by a post-magnetization process based on chemical coprecipitation, via immersing the produced fibrous mats in an aqueous solution containing Fe(II) and Fe(III) salts at appropriate molar ratios, followed by the addition of a weak base to yield MNPs. Electron microscopy revealed the presence of continuous micron and submicron fibers surface-decorated with MNPs. The magnetically modified PVP/CS fibers exhibited superparamagnetic behavior at ambient temperature. The magnetic fibrous nanocomposite carrier was employed for the immobilization of Saccharomyces cerevisiae cells and their use for sucrose hydrolysis, and Candida rugosa lipase and its use for artificial substrate hydrolysis.


[1] P. Supaphol, O. Suwantong, P. Sangsanoh, S. Srinivasan, R. Jayakumar, S.V. Nair, Electrospinning of biocompatible polymers and their potentials in biomedical applications, Advances in Polymer Science 246, 2012, 213-239.10.1007/12_2011_143Search in Google Scholar

[2] J.Y. Yang, S.H. Zhan, N.Wang, X.M.Wang, Y. Li, Y. Li,W.Y.Ma, H.B. Yu, A mini review: Electrospun hierarchical nanofibers, Journal of Dispersion Science and Technology 31, 2010, 760-769.10.1080/01932690903333127Search in Google Scholar

[3] D.G. Yu, L.M. Zhu, K. White, C. Branford-White, Electrospun nanofiber-based drug delivery systems, Health 1, 2009, 67-75.10.4236/health.2009.12012Search in Google Scholar

[4] Y. Tian, M.Wu, R. Liu, Y. Li, D.Wang, J. Tan, R.Wu, Y. Huang, Electrospun membrane of cellulose acetate for heavy metal ion adsorption in water treatment, Carbohydrate Polymers 83, 2011, 743-748.10.1016/j.carbpol.2010.08.054Search in Google Scholar

[5] X.Y. Ye, Z.M. Liu, Z.G. Wang, X.J. Huang, Z.K. Xu, Preparation and characterization of magnetic nanofibrous composite membranes with catalytic activity, Materials Letters 63, 2009, 1810-1813.10.1016/j.matlet.2009.05.054Search in Google Scholar

[6] Z.-G. Wang, L.-S. Wan, Z.-M. Liu, X.-J. Huang, Z.-K. Xu, Enzyme immobilization on electrospun polymer nanofibers: An overview, Journal of Molecular Catalysis B: Enzymatic 56, 2009, 189-195.10.1016/j.molcatb.2008.05.005Search in Google Scholar

[7] M.F. Canbolat, N. Gera, C. Tang, B. Monian, B.M. Rao, B. Pourdeyhimi, S.A. Khan, Preservation of cell viability and protein conformation on immobilization within nanofibers via electrospinning functionalized yeast, ACS AppliedMaterials&Interfaces 5 (19), 2013, 9349-9354.10.1021/am4022768Search in Google Scholar PubMed

[8] A.C. Mendes, K. Stephansen, I.S. Chronakis, Electrospinning of food proteins and polysaccharides, Food Hydrocolloids 68, 2017, 53-68.10.1016/j.foodhyd.2016.10.022Search in Google Scholar

[9] R. Sridhar, R. Lakshminarayanan, K. Madhaiyan, V.A. Barathi, K.H.C. Limh, S. Ramakrishna, Electrosprayed nanoparticles and electrospun nanofibers based on natural materials: applications in tissue regeneration, drug delivery and pharmaceuticals, Chemical Society Reviews 44, 2015, 790-814.10.1039/C4CS00226ASearch in Google Scholar PubMed

[10] M. Kong, X.G. Chen, K. Xing, H.J. Park, Antimicrobial properties of chitosan and mode of action: A state of the art review, Internatioanal Journal of Food Microbiology 144, 2010, 51-63.10.1016/j.ijfoodmicro.2010.09.012Search in Google Scholar PubMed

[11] K. Sun, Z.H. Li, Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning, Express Polymer Letters 5, 2011, 342-361.10.3144/expresspolymlett.2011.34Search in Google Scholar

[12] Y.S. Zhou, D.Z. Yang, X.M. Chen, Q. Xu, F.M. Lu, J. Nie, Electrospun water-soluble carboxyethyl chitosan/poly(vinyl alcohol) nanofibrous membrane as potential wound dressing for skin regeneration, Biomacromolecules 9, 2008, 349-354.10.1021/bm7009015Search in Google Scholar PubMed

[13] J.P. Chen, G.Y. Chang, J.K. Chen, Electrospun collagen/chitosan nanofibrous membrane as wound dressing, Colloids and Surfaces A 313, 2008, 183-188.10.1016/j.colsurfa.2007.04.129Search in Google Scholar

[14] F. Croisier, C. Jerome, Chitosan-based biomaterials for tissue engineering, European Polymer Journal 49, 2013, 780-792.10.1016/j.eurpolymj.2012.12.009Search in Google Scholar

[15] M.G. Lancina, R.K. Shankar, H. Yang, Chitosan nanofibers for transbuccal insulin delivery, Journal of Biomedical Materals Research A 105, 2017, 1252-1259.10.1002/jbm.a.35984Search in Google Scholar PubMed PubMed Central

[16] R. Sedghi, A. Shaabani, Z. Mohammadi, F.Y. Samadi, E. Isaei, Biocompatible electrospinning chitosan nanofibers: A novel delivery system with superior local cancer therapy, Carbohydrate Polymers 159 (2017) 1-10.Search in Google Scholar

[17] W. Tighzert, A. Habi, A. Ajji, T. Sadoun, F.B.O. Daoud, Fabrication and characterization of nanofibers based on poly(lactic acid)/chitosan blends by electrospinning and their functionalization with phospholipase A1, Fibers and Polymers 18, 2017, 514-524.10.1007/s12221-017-6953-xSearch in Google Scholar

[18] N.M. Siqueira, K.C. Garcia, R. Bussamara, F.S. Both, M.H. Vainstein, R.M.D. Soares, Poly (lactic acid)/chitosan fiber mats: Investigation of effects of the support on lipase immobilization, International Journal of Biological Macromolecules 72, 2015, 998-1004.10.1016/j.ijbiomac.2014.08.048Search in Google Scholar PubMed

[19] P. Wen, K. Feng, H. Yang, X. Huang, M.H. Zong, W.Y. Lou, N. Li, H. Wu, Electrospun core-shell structured nanofilm as a novel colon-specific delivery system for protein, Carbohydrate Polymers 169, 2017, 157-166.10.1016/j.carbpol.2017.03.082Search in Google Scholar PubMed

[20] T.C. Mokhena, A.S. Luyt, Development of multifunctional nano/ultrafiltration membrane based on a chitosan thin film on alginate electrospun nanofibres, Journal of Cleaner Production 156, 2017, 470-479.10.1016/j.jclepro.2017.04.073Search in Google Scholar

[21] I. Savva, T. Krasia-Christoforou, Electrospun magnetoactive fibrous nanocomposites: Fabrication and applications in biomedicine. In: N.P. Sabbas (Ed.)Magnetic Nanoparticles: Synthesis, Physicochemical Properties and Role in Biomedicine. Nova Science Publishers, 2014, pp. 163-200.Search in Google Scholar

[22] H.T. Wang, Y.H. Chan, S.W. Feng, Y.J. Lo, N.C. Teng, H.M. Huang, Development and biocompatibility tests of electrospun poly-Llactide nanofibrous membranes incorporating oleic acid-coated Fe3O4, Journal of Polymer Engineering 34, 2014, 241-245.10.1515/polyeng-2013-0206Search in Google Scholar

[23] L. Li, G. Yang, J. Li, S. Ding, S. Zhou, Cell behaviors on magnetic electrospun poly-d, l-lactide nanofibers, Materials Science and Engineering C 34, 2014, 252-261.10.1016/j.msec.2013.09.021Search in Google Scholar PubMed

[24] F. Sharifi, A.C. Sooriyarachchi, H. Altural, R. Montazami, M.N. Rylander, N. Hashemi, Fiber based approaches as medicine delivery systems, ACS Biomaterials Science & Engineering 2, 2016, 1411-1431.10.1021/acsbiomaterials.6b00281Search in Google Scholar PubMed

[25] L.G.Wang, M.Wang, P.D. Topham, Y. Huang, Fabrication ofmagnetic drug-loaded polymeric composite nanofibres and their drug release characteristics, RSC Advances 2, 2012, 2433-2438.10.1039/c2ra00484dSearch in Google Scholar

[26] I. Savva, A.D. Odysseos, L. Evaggelou, O. Marinica, E. Vasile, L. Vekas, Y. Sarigiannis, T. Krasia-Christoforou, Fabrication, characterization, and evaluation in drug release properties of magnetoactive poly(ethylene oxide)-poly(L-lactide) electrospun membranes, Biomacromolecules 14, 2013, 4436-4446.10.1021/bm401363vSearch in Google Scholar PubMed

[27] T.C. Lin, F.H. Lin, J.C. Lin, In vitro feasibility study of the use of a magnetic electrospun chitosan nanofiber composite for hyperthermia treatment of tumor cells, Acta Biomaterialia 8, 2012, 2704-2711.10.1016/j.actbio.2012.03.045Search in Google Scholar PubMed

[28] T.C.Lin, F.H. Lin, J.C. Lin, In vitro characterization of magnetic electrospun IDA-grafted chitosan nanofiber composite for hyperthermic tumor cell treatment, Journal of Biomaterials Science, Polymer Edition 24, 2013, 1152-1163.10.1080/09205063.2012.743061Search in Google Scholar PubMed

[29] I. Savva, A. S. Kalogirou, A. Chatzinicolaou, P. Papaphiippou, A. Pantelidou, E. Vasile, Eu. Vasile, P.A. Koutentis, T. Krasia-Christoforou, PVP-crosslinked electrospun membranes with embedded Pd and Cu2O nanoparticles as effective heterogeneous catalytic supports, RSC Advances, 4, 2014, 44911-44921.10.1039/C4RA06294ASearch in Google Scholar

[30] S. Laurent, D. Forge, M. Port, A. Roch, C. Robic, L.V. Elst, R.N. Muller,Magnetic iron oxide nanoparticles: Synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications, Chemical Reviews 108, 2008, 2064-2110.10.1021/cr068445eSearch in Google Scholar PubMed

[31] N. Bhattarai, D. Edmondson, O. Veiseh, F.A. Matsen, M.Q. Zhang, Electrospun chitosan-based nanofibers and their cellular compatibility, Biomaterials 26, 2005, 6176-6184.10.1016/j.biomaterials.2005.03.027Search in Google Scholar PubMed

[32] I. Savva, M. Efstathiou, T. Krasia-Christoforou, I. Pashalidis, Adsorptive removal of U(VI) and Th(IV) from aqueous solutions using polymer-based electrospun PEO/PLLA fibrous membranes, Journal of Radioanalytical and Nuclear Chemistry 298, 2013, 1991-1997.10.1007/s10967-013-2657-xSearch in Google Scholar

[33] S.R. Mao, X.T. Shuai, F. Unger, M. Simon, D.Z. Bi, T. Kissel, The depolymerization of chitosan: effects on physicochemical and biological properties, International Journal of Pharmaceutics 281, 2004, 45-54.10.1016/j.ijpharm.2004.05.019Search in Google Scholar PubMed

[34] A.E. Jablonski, A.J. Lang, S. Vyazovkin, Isoconversional kinetics of degradation of polyvinylpyrrolidone used as a matrix for ammonium nitrate stabilization, Thermochimica Acta 474 (2008) 78-80.10.1016/j.tca.2008.06.003Search in Google Scholar

[35] R.W. Chantrell, J. Popplewell, S.W. Charles, Measurements of particle-size distribution parameters in ferrofluids, IEEE Transactions on Magnetics 14, 1978, 975-977.10.1109/TMAG.1978.1059918Search in Google Scholar

[36] R.E. Rosensweig, Ferrohydrodynamics, Dover Publications, Inc., 2014.Search in Google Scholar

Received: 2018-06-06
Accepted: 2018-08-04
Published Online: 2018-09-25
Published in Print: 2018-09-01

© by Ivo Safarik et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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