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Chemistry and kraft pulping of seven hybrid aspen clones. Dimension measurements on the vessels and UMSP of the cell walls

  • Inese Sable , Uldis Grinfelds , Martins Zeps , Ilze Irbe , Guna Noldt , Aris Jansons , Arnis Treimanis EMAIL logo and Gerald Koch
From the journal Holzforschung


Micropropagated hybrid aspen clones (Populus tremuloides Michx.×Populus tremula L.) and a plus-tree (superior phenotypes selected) aspen (Populus tremula L.) were grown under similar conditions in the central part of Latvia. After cutting at the age of 12 years, 64 sample trees were examined. The dimensions of the vessels and the content of cellulose, lignin, extractives and ash were determined. The cell walls were characterised by UV microspectrophotometry (UMSP) in the UV range. The dimensions of fibres obtained by kraft pulping were determined such as length, width, and their shape factors. Coarseness measurements were performed, and strength properties of the handsheets were tested. One of the clones (no. 44) had a significantly higher stem volume of 208 dm3 and stem diameter of 17 cm at a height of 1.3 m. This clone had otherwise no notable differences to the other clones concerning the chemical composition, except a slightly higher lignin content (20.0% as compared with the mean value of 19.3% for other clones). Kraft cooking of hybrid aspen chips gave pulp yields in the range of 48.6–52.4%. Slightly higher strength properties (10–15%) were found for the handsheets prepared from a common aspen pulp (reference) as compared with those from hybrid aspen fibres.

Corresponding author: Arnis Treimanis, Latvian State Institute of Wood Chemistry, 27 Dzerbenes Str., LV-1006 Riga, Latvia, Phone: +371 29258122, Fax: +371 67550635

This research was accomplished in the framework of the ESF project No. 2009/0200/1DP/ “Importance of Genetic Factors in Formation of Forest Stands with High Adaptability and Qualitative Wood Properties”. We would like to thank Dr Uwe Noldt, Johann Heinrich von Thünen-Institute (vTI)/Federal Research Institute for Rural Areas, Forestry and Fisheries Institute of Wood Technology and Wood Biology (HTB), Hamburg, Germany for his assistance in writing individual sections.


Balatinecz, J.J., Kretschmann, D.E. (2001) Properties and utilization of poplar wood. In: Poplar Culture in North America. Part A. Eds. Dickmann, D.I., Isebrands, J.G., Eckenwalder, J.E., Richardson, J. NRC Research Press, National Research Council of Canada, Ottawa. pp. 277–291.Search in Google Scholar

Donaldson, L.A. (2001) Lignification and lignin topochemistry – an ultrastructural view. Phytochemistry 57:859–873.10.1016/S0031-9422(01)00049-8Search in Google Scholar

Donaldson, L.A., Hague, J., Snell, R. (2001) Lignin distribution in coppice poplar, linseed and wheat straw. Holzforschung 55:379–385.10.1515/HF.2001.063Search in Google Scholar

Fergus, B.J., Goring, D.A.I. (1970) The location of guaiacyl and syringyl lignins in birch xylem tissue. Holzforschung 24:113–117.10.1515/hfsg.1970.24.4.113Search in Google Scholar

Francis, R.C., Hanna, R.B., Shin, S.-J., Brown, A.F., Riemenschneider, D.E. (2006) Papermaking characteristics of three Populus clones grown in the north-central United States. Biomass Bioenerg. 30:803–808.10.1016/j.biombioe.2005.08.003Search in Google Scholar

Fukazawa, K. (1992) Ultraviolet microscopy. In: Methods in Lignin Chemistry. Eds. Lin, S.Y., Dence, C.W. Springer-Verlag, Berlin. pp. 110–121.10.1007/978-3-642-74065-7_8Search in Google Scholar

Grünwald, C., Ruel, K., Schmitt, U. (2002) Differentiation of xylem cells in rolC transgenic aspen trees – a study of secondary cell wall development. Ann. Forest Sci. 59: 679–685.Search in Google Scholar

Horváth, L., Peszlén, I., Gierlinger, N., Peralta, P., Kelley, S., Csóka, L. (2012) Distribution of wood polymers within the cell wall of transgenic aspen imaged by Raman microscopy. Holzforschung 66:717–725.10.1515/hf-2011-0126Search in Google Scholar

Koch, G., Grünwald, C. (2004) Application of UV microspectrophotometry for the topochemical detection of lignin and phenolic extractives in wood fibre cell walls. In: Wood Fibre Cell Walls: Methods to Study their Formation, Structure and Properties. Ed. Schmitt, U. Swedish University of Agricultural Sciences, Uppsala. pp. 119–130.Search in Google Scholar

Koch, G., Kleist, G. (2001) Application of scanning UV microspectrophotometry to localise lignins and phenolic extractives in plant cell walls. Holzforschung 55:563–567.10.1515/HF.2001.091Search in Google Scholar

Koch, G., Rose, B., Patt, R., Kordsachia, O. (2003) Topochemical investigations on delignification of Picea abies [L.] Karst. during alkaline sulfite (ASA) and bisulfite pulping by scanning UV microspectrophotometry. Holzforschung 57:611–618.10.1515/HF.2003.092Search in Google Scholar

Lehringer, C., Gierlinger, N., Koch, G. (2008) Topochemical investigation on tension wood fibres of Acer spp., Fagus sylvatica L. and Quercus robur L. Holzforschung 62:255–263.10.1515/HF.2008.036Search in Google Scholar

Lehringer, C., Koch, G., Adusumalli, R.-B., Mook, W.M., Richter, K., Militz, H. (2011) Effect of Physisporinus vitreus on wood properties of Norway spruce. Part 1: Aspects of delignification and surface hardness. Holzforschung 65:711–719.10.1515/hf.2011.021Search in Google Scholar

Luo, Z.B., Langenfeld-Heyser, R., Calfapietra, C., Polle, A. (2005) Influence of free air CO2 enrichment (EUROFACE) and nitrogen fertilisation on the anatomy of juvenile wood of three poplar species after coppicing. Trees 19:109–118.10.1007/s00468-004-0369-0Search in Google Scholar

McDonough, T.J., Malcolm, E.W., Einspahr, D.W. (1985) Factors affecting the outlook for utilization of hardwoods in pulping and papermaking. IPC Technical Report Paper Series, No 154. Appleton, Wisconsin.Search in Google Scholar

Morikawa, Y., Yoshinaga, A., Kamitakahara, H., Wada, M., Takabe, K. (2010) Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy. Holzforschung 64:61–67.10.1515/hf.2010.015Search in Google Scholar

Musha, Y., Goring, D.A.I. (1975) Distribution of syringyl and guaiacyl moieties in hardwoods as indicated by ultraviolet microscopy. Wood Sci. Technol. 9:45–58.Search in Google Scholar

Prislan, P., Koch, G., Čufar, K., Gričar, J., Schmitt, U. (2009) Topochemical investigations of cell walls in developing xylem of beech (Fagus sylvatica L.). Holzforschung 63:482–490.10.1515/HF.2009.079Search in Google Scholar

Prislan, P., Koch, G., Schmitt, U., Gričar, J., Čufar, K. (2012) Cellular and topochemical characteristics of secondary changes in bark tissues of beech (Fagus sylvatica). Holzforschung 66:131–138.10.1515/HF.2011.119Search in Google Scholar

Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31–43.Search in Google Scholar

Terashima, N., Fukushima, K., Takabe, K. (1986) Heterogeneity in formation of lignin. VIII. An autoradiographic study on the formation of guaiacyl and syringyl lignin in Magnolia kobus DC. Holzforschung 40:101–105.Search in Google Scholar

Terashima, N., Fukushima, K., Takabe, K. (1993) Comprehensive model of the lignified plant cell wall. In: Forage Cell Wall Structure and Digestibility. Eds. Jung, H.G., Buxton, D.R., Hatfield, R.D., Ralph, J. American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc., Madison. pp. 247–270.10.2134/1993.foragecellwall.c10Search in Google Scholar

Terashima, N., Yoshida, M., Hafrén, J., Fukushima, K., Westermark, U. (2012) Proposed supramolecular structure of lignin in softwood tracheid compound middle lamella regions. Holzforschung 66:907–915.10.1515/hf-2012-0021Search in Google Scholar

Treimanis, A., Grinfelds, U., Skute, M., Gailis, A., Zeps, M. (2006) Comparative study of wood and pulp fibres obtained from natural forest and plantation aspen trees. In: Proceeding of 9th European Workshop on Lignocellulosics and Pulp, August 27–30, Vienna, Austria. pp. 561–563.Search in Google Scholar

Tullus, A., Tullus, H., Vares, A., Kanal, A. (2007) Early growth of hybrid aspen (Populus x wettsteinii Hamet-Ahti) plantations on former agricultural lands in Estonia. Forest Ecol. Manag. 245:118–129.Search in Google Scholar

Tullus, A., Rytter, L., Tullus, T., Weih, M., Tullus, H. (2012) Short-rotation forestry with hybrid aspen (Populus tremula L. x P. tremuloides Michx.) in Northern Europe. Scand. J. Forest Res. 27:10–29.Search in Google Scholar

Yu, Q., Tigerstedt, P.M.A., Haapanen, M. (2001) Growth and phenology of hybrid aspen clones (Populus tremula L. x Populus tremuloides Michx.). Silva Fenn. 35:15–25.10.14214/sf.600Search in Google Scholar

Zeps, M., Auzenbaha, D., Gailis, A., Treimanis, A., Grīnfelds, U. (2008) Evaluation and selection of hybrid aspen (Populus tremuloides X Populus tremula) clones. Mežzinātne (Forest Science). 18:19–34 (in Latvian, summary in English).Search in Google Scholar

Received: 2012-10-31
Accepted: 2013-1-4
Published Online: 2013-02-11
Published in Print: 2013-07-01

©2013 by Walter de Gruyter Berlin Boston

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