Accessible Requires Authentication Published by De Gruyter August 19, 2020

Comprehensive study on the effects of process parameters of alkaline thermal pretreatment followed by thermomechanical extrusion in sugar liberation from Eucalyptus grandis wood

Pablo Doménech ORCID logo, Paloma Manzanares, Cristina Álvarez, Mercedes Ballesteros and Aleta Duque
From the journal Holzforschung

Abstract

A combination of alkaline thermal pretreatment followed by thermomechanical extrusion was studied as a novel sequential pretreatment process for an effective breakdown of the lignocellulosic structure of Eucalyptus grandis wood (EW). The first step was studied by analysing the influence of two factors: the NaOH-to-dry biomass ratio or NaOH loading (NaOH/DM) and the liquid-to-solid ratio (L/S). Optimization of these two parameters provided good results in terms of enzymatic hydrolysis at 5% (w w−1) solids loading, obtaining a total sugar concentration of 24.9 g L−1 and a total sugar production of 36.9 g 100 g−1 raw EW after pretreating the biomass at 20% NaOH/DM and L/S = 1/1. The second step of extrusion, when followed by a final washing step, provided a significant increase in glucose and xylose production when working at 10% NaOH/DM. For a soda loading of 20%, there was a clear improvement in sugars conversion yield after extrusion and washing: 71% for glucan conversion and 89% for xylan.


Corresponding author: Pablo Doménech, Biofuels Unit, Energy Department – CIEMAT, Avda. Complutense 40, 28040, Madrid, Spain, E-mail:

Funding source: European Commission

Award Identifier / Grant number: 654365

Acknowledgments

The authors would like to thank the INIA (Uruguay) for kindly providing the eucalyptus biomass used for all experiments and Novozymes A/S (Denmark) for kindly providing the enzymatic cocktail used for saccharification.

  1. Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was carried out in the frame of the BABET-REAL5 Project. The project is co-funded by the European Union within the Horizon 2020 programme Grant Agreement No. 654365.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

References

Carvalho, D.M., de Queiroz, J.H., and de Colodette, J.L. (2016). Assessment of alkaline pretreatment for the production of bioethanol from eucalyptus, sugarcane bagasse and sugarcane straw. Ind. Crop. Prod. 94: 932–941, https://doi.org/10.1016/j.indcrop.2016.09.069. Search in Google Scholar

Carvalho, D.M., deQueiroz, J.H., and deLuiz, J. (2017). Hydrothermal and acid pretreatments improve ethanol production from lignocellulosic biomasses. Bio Resources 12: 3088–3107, https://doi.org/10.15376/BIORES.12.2.3088-3107. Search in Google Scholar

Carvalho, D.M., de Sevastyanova, O., Penna, L.S., Silva, B.P. daLindström, M.E., and Colodette, J.L. (2015). Assessment of chemical transformations in eucalyptus, sugarcane bagasse and straw during hydrothermal, dilute acid, and alkaline pretreatments. Ind. Crop. Prod. 73: 118–126. Search in Google Scholar

Duque, A., Manzanares, P., Ballesteros, I., Negro, M.J., Oliva, J.M., Saez, F., and Ballesteros, M. (2014). Study of process configuration and catalyst concentration in integrated alkaline extrusion of barley straw for bioethanol production. Fuel 134: 448–454, https://doi.org/10.1016/j.fuel.2014.05.084. Search in Google Scholar

Duque, A., Manzanares, P., Ballesteros, I., Negro, M.J., Oliva, J.M., Saez, F., and Ballesteros, M. (2013). Optimization of integrated alkaline-extrusion pretreatment of barley straw for sugar production by enzymatic hydrolysis. Process Biochem. 48: 775–781, https://doi.org/10.1016/j.procbio.2013.03.003. Search in Google Scholar

Duque, A., Manzanares, P., and Ballesteros, M. (2017). Extrusion as a pretreatment for lignocellulosic biomass: fundamentals and applications. Renew. Energy 114: 1427–1441, https://doi.org/10.1016/j.renene.2017.06.050. Search in Google Scholar

Duque, A., Manzanares, P., González, A., and Ballesteros, M. (2018). Study of the application of alkaline extrusion to the pretreatment of Eucalyptus biomass as first step in a bioethanol production process. Energies 11: 2961, https://doi.org/10.3390/en11112961. Search in Google Scholar

Gigac, J., Fišerová, M., Stankovská, M., and Pazitný, A. (2017). Enzymatic hydrolysis of extruded wheat straw with addition of sodium hydroxide and calcium hydroxide. Wood Res. 62: 919–929, http://www.woodresearch.sk/wr/201706/09.pdf. Search in Google Scholar

Guigou, M., Cabrera, M.N., Vique, M., Bariani, M., Guarino, J., Ferrari, M.D., and Lareo, C. (2019). Combined pretreatments of eucalyptus sawdust for ethanol production within a biorefinery approach. Biomass Convers. Biorefinery 9: 293–304, https://doi.org/10.1007/s13399-018-0353-3. Search in Google Scholar

Hodge, D.B., Karim, M.N., Schell, D.J., and McMillan, J.D. (2008). Soluble and insoluble solids contributions to high-solids enzymatic hydrolysis of lignocellulose. Bioresour. Technol. 99: 8940–8948, https://doi.org/10.1016/j.biortech.2008.05.015. Search in Google Scholar

Huo, D., Yang, Q., Fang, G., Liu, Q., Si, C., Hou, Q., and Li, B. (2018). Improving the efficiency of enzymatic hydrolysis of Eucalyptus residues with a modified aqueous ammonia soaking method. Nord. Pulp Pap Res. J. 33: 165–174, https://doi.org/10.1515/npprj-2018-3025. Search in Google Scholar

Isikgor, F.H., and Becer, C.R. (2015). Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers. Polym. Chem. 6: 4497–4559, https://doi.org/10.1039/c5py00263j. Search in Google Scholar

Jørgensen, H., Kristensen, J.B., and Felby, C. (2007). Enzymatic conversion of lignocellulose into fermentable sugars: challenges and opportunities. Biofuels Bioprod. Biorefining 1: 119–134, https://doi.org/10.1002/bbb.4. Search in Google Scholar

Kim, J.S., Lee, Y.Y., and Kim, T.H. (2016). A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresour. Technol. 199: 42–48, https://doi.org/10.1016/j.biortech.2015.08.085. Search in Google Scholar

Kristensen, J.B., Felby, C., and Jørgensen, H. (2009). Determining yields in high solids enzymatic hydrolysis of biomass. Appl. Biochem. Biotechnol. 156: 127–132, https://doi.org/10.1007/s12010-008-8375-0. Search in Google Scholar

Liu, H.M., Li, H.Y., and Wei, A.C. (2017). Enhanced polysaccharides yield obtained from hydrothermal treatment of corn bran via twin-screw extrusion. Bio Resources 12: 3933–3947, https://doi.org/10.15376/biores.12.2.3933-3947. Search in Google Scholar

Lu, H., Zhang, X., Wu, A., Deng, X., Ren, J., Kong, F., and Li, H. (2017). Comparison of dilute acid, alkali, and biological pretreatments for reducing sugar production from eucalyptus. Bio Resources 12: 6353–6365, https://doi.org/10.15376/biores.12.3.6353-6365. Search in Google Scholar

Merrettig-Bruns, U., and Sayder, B. (2016). Pretreatment with ammonia. In: Biomass fractionation technologies for a lignocellulosic feedstock based biorefinery, https://doi.org/10.1016/B978-0-12-802323-5.00020-7. Search in Google Scholar

Murciano Martínez, P., Bakker, R., Harmsen, P., Gruppen, H., and Kabel, M. (2015). Importance of acid or alkali concentration on the removal of xylan and lignin for enzymatic cellulose hydrolysis. Ind. Crop. Prod. 64: 88–96, https://doi.org/10.1016/j.indcrop.2014.10.031. Search in Google Scholar

Negro, M.J., Duque, A., Manzanares, P., Sáez, F., Oliva, J.M., Ballesteros, I., and Ballesteros, M. (2015). Alkaline twin-screw extrusion fractionation of olive-tree pruning biomass. Ind. Crop. Prod. 74: 336–341, https://doi.org/10.1016/j.indcrop.2015.05.018. Search in Google Scholar

Park, Y.C., and Kim, J.S. (2012). Comparison of various alkaline pretreatment methods of lignocellulosic biomass. Energy 47: 31–35, https://doi.org/10.1016/j.energy.2012.08.010. Search in Google Scholar

Romaní, A., Garrote, G., Ballesteros, I., and Ballesteros, M. (2013). Second generation bioethanol from steam exploded Eucalyptus globulus wood. Fuel 111: 66–74, https://doi.org/10.1016/j.fuel.2013.04.076. Search in Google Scholar

Sixta, H. (2006). Introduction. In: Sixta, H. (Ed.). Handbook of pulp. Wiley-VCH Verlag GmbH, Weinheim, Germany, pp. 2–19, https://doi.org/10.1002/9783527619887.ch1. Search in Google Scholar

Sluiter, A., Hyman, D., Payne, C., Wolfe, J., Hames, B., Hyman, D., Payne, C., Ruiz, R., Scarlata, C., Sluiter, J., et al. (2008). Determination of structural carbohydrates and lignin in biomass [Report No. NREL/TP-510-42618]. Golden, CO, USA: National Renewable Energy Laboratory. https://doi.org/nrel.gov/docs/gen/fy13/42618.pdf. Search in Google Scholar

Sudiyani, Y., Triwahyuni, E., MuryantoBurhani, D., Waluyo, J., Sulaswaty, A., and Abimanyu, H. (2016). Alkaline pretreatment of sweet sorghum bagasse for bioethanol production. Int. J. Renew. Energy Dev. 5: 113–118, https://doi.org/10.14710/ijred.5.2.113-118. Search in Google Scholar

Sun, S.N., Li, H.Y., Cao, X.F., Xu, F., and Sun, R.C. (2015). Structural variation of eucalyptus lignin in a combination of hydrothermal and alkali treatments. Bioresour. Technol. 176: 296–299, https://doi.org/10.1016/j.biortech.2014.11.030. Search in Google Scholar

Sun, S., Cao, X., Sun, S., Xu, F., Song, X., Sun, R.C., and Jones, G.L. (2014). Improving the enzymatic hydrolysis of thermo-mechanical fiber from Eucalyptus urophylla by a combination of hydrothermal pretreatment and alkali fractionation. Biotechnol. Biofuels 7: 1–12, https://doi.org/10.1186/s13068-014-0116-8. Search in Google Scholar

Tao, L., Ren, J., Yu, F.K., and Ni, T.R. (2015). Effects of liquid-to-solid ratio and reaction time on dilute sulfuric acid pretreatment of Achnatherum splendens. Asian J. Chem. 27: 2133–2136, https://doi.org/10.14233/ajchem.2015.17803. Search in Google Scholar

Uzuner, S., Sharma Shivappa, R.R., and Cekmecelioglu, D. (2017). Bioconversion of alkali pretreated hazelnut shells to fermentable sugars for generation of high value products. Waste Biomass Valorizat. 8: 407–416, https://doi.org/10.1007/s12649-016-9607-0. Search in Google Scholar

Vargas, R., Kowalski, V., and Vecchietti, A. (2016). Fermentable sugars from Eucalyptus globulus: process optimization. Comput. Chem. Eng. 93: 343–352, https://doi.org/10.1016/j.compchemeng.2016.07.012. Search in Google Scholar

Wang, Q., Wang, W., Tan, X., Zahoor, Chen, X., Guo, Y., Yu, Q., Yuan, Z., and Zhuang, X. (2019). Low-temperature sodium hydroxide pretreatment for ethanol production from sugarcane bagasse without washing process. Bioresour. Technol. 291: 121844, https://doi.org/10.1016/j.biortech.2019.121844. Search in Google Scholar

Xu, H., Li, B., and Mu, X. (2016). Review of alkali-based pretreatment to enhance enzymatic saccharification for lignocellulosic biomass conversion. Ind. Eng. Chem. Res. 55: 8691–8705, https://doi.org/10.1021/acs.iecr.6b01907. Search in Google Scholar

Xu, J., and Cheng, J.J. (2011). Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime. Bioresour. Technol. 102: 3861–3868, https://doi.org/10.1016/j.biortech.2010.12.038. Search in Google Scholar

Yamakawa, C.K., Qin, F., and Mussatto, S.I. (2018). Advances and opportunities in biomass conversion technologies and biorefineries for the development of a bio-based economy. Biomass Bioenergy 119: 54–60, https://doi.org/10.1016/j.biombioe.2018.09.007. Search in Google Scholar

Zhao, X., Wu, R., and Liu, D. (2011). Production of pulp, ethanol and lignin from sugarcane bagasse by alkali-peracetic acid delignification. Biomass Bioenergy 35: 2874–2882, https://doi.org/10.1016/j.biombioe.2011.03.033. Search in Google Scholar

Received: 2020-03-11
Accepted: 2020-07-01
Published Online: 2020-08-19
Published in Print: 2021-03-26

© 2020 Walter de Gruyter GmbH, Berlin/Boston