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Licensed Unlicensed Requires Authentication Published by De Gruyter November 29, 2011

Fractionation and characterization of lignin carbohydrate complexes (LCCs) of Eucalyptus globulus in residues left after MWL isolation. Part I: Analyses of hemicellulose-lignin fraction (HC-L)

Yasuyuki Miyagawa, Ooki Takemoto, Toshiyuki Takano, Hiroshi Kamitakahara and Fumiaki Nakatsubo
From the journal

Abstract

The residual wood meal left after milled wood lignin (MWL) isolation (MWR) was extracted with the cellulose solvent lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) to obtain a soluble fraction (C-L) and an insoluble fraction (C-L-residue). The C-L-residue was further extracted with the hemicellulose solvent 3 M NaOH to give a soluble fraction named hemicellulose-lignin fraction (HC-L) with 21.3% yield based on MWR. It was found that HC-L was composed of xylan, cellulose and lignin with abundant S-type β-O-4 substructures. HC-L lignin was bonded to HC-L cellulose or HC-L hemicelluloses or both. The method, which comprised acetylation for hardwood xylan (by acetic anhydride/pyridine/formamide) and extraction with chloroform, was found to be effective for selective xylan acetate fractionation. HC-L was further fractionated by the same method and subsequent deacetylation to give a xylan-lignin fraction (X-L) in 11.3% yield based on HC-L. X-L was composed mainly of xylan and lignin with abundant S-type β-O-4 substructures, and bonded to X-L xylan. X-L is considered as a promising fraction for elucidation of the structure of lignin-carbohydrate linkages.


Corresponding author. Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan Phone: +81-75-753-6254 Fax: +81-75-753-6300

Received: 2011-5-22
Accepted: 2011-10-24
Published Online: 2011-11-29
Published in Print: 2012-05-01

©2012 by Walter de Gruyter Berlin Boston

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