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Licensed Unlicensed Requires Authentication Published by De Gruyter April 11, 2022

Serum 25(OH) vitamin D level in Relapsing-Remitting Multiple Sclerosis and clinically isolated syndrome groups

Ahmet Küçük ORCID logo , Levent Sinan Bir ORCID logo , Selma Tekin ORCID logo EMAIL logo and Süleyman Demir ORCID logo



Vitamin D is an important regulator of the immune system, and it has been shown that deficiency of vitamin D is significant environmental factor in some immune-mediated diseases such as Multiple Sclerosis (MS). In this study, we have compared serum 25 hydroxyvitamin (OH) D levels in Relapsing-Remitting Multiple Sclerosis (RRMS), clinically isolated syndrome (CIS), and control groups.


Forty patients with CIS and 60 patients who have been diagnosed RRMS between age 18–45, respectively, and followed up at Pamukkale University Faculty of Medicine, Department of Neurology, and 60 healthy individuals have been included in this study. Serum 25(OH) vitamin D, calcium, phosphorus, alkaline phosphatase, parathormone, insulin, and fasting blood glucose levels were studied for all three groups.


A statistically significant difference was determined in the comparison of three groups for mean 25(OH) vitamin D levels. In the intergroup comparison of mean 25(OH) vitamin D; mean 25(OH) vitamin D level was determined to be statistically significantly lower in both RRMS and CIS groups compared to control group (p<0.05).


Since vitamin D deficiency poses a problem from the early stage of disease spectrum in both CIS patients and MS patients, 25(OH) vitamin D level should be routinely controlled, and replacement should be administered upon any deficiency state.

Corresponding author: Dr. Selma Tekin, MD, Department of Neurology, Faculty of Medicine, Pamukkale University, Denizli, Turkey, Phone: +0090 4440728/5663, E-mail:
Current affiliation: Ahmet Küçük, Department of Neurology, Beyhekim State Hospital, Konya, Turkey.

Funding source: Pamukkale University Scientific Research Projects Coordination Unit

Award Identifier / Grant number: 2014TPF002

  1. Research funding: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Pamukkale University Scientific Research Projects Coordination Unit (BAP) [2014TPF002].

  2. Author contributions: AK designed the study, AK analyzed the data under supervision of LSB, ST processed data, SD and ST are the co-principal investigators, AK and ST reviewed LSB the literature and AK wrote the manuscript.

  3. Competing interests: The authors declare that there is no conflict of interest.

  4. Informed consent: All participants provided written informed consent before the study.

  5. Ethical approval: The study was approved by the Pamukkale University Ethics Committee for non-interventional clinical research (27082013#12).


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Received: 2021-08-13
Accepted: 2022-03-12
Published Online: 2022-04-11

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