Accessible Requires Authentication Published by De Gruyter November 13, 2012

Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes

Bettina Grismayer, Sumito Sato, Charlotte Kopitz, Christian Ries, Susanne Soelch, Manfred Schmitt, Gustavo Baretton, Achim Krüger, Thomas Luther, Matthias Kotzsch and Viktor Magdolen
From the journal Biological Chemistry

Abstract

mRNA levels of the urokinase receptor splice variant uPAR-del4/5 are associated with prognosis in breast cancer. Its overexpression in cancer cells affects tumor biologically relevant processes. In the present study, individual breast cancer cell clones displaying low vs. high uPAR-del4/5 expression were analyzed demonstrating that uPAR-del4/5 leads to reduced cell adhesion and invasion in a dose-dependent manner. Additionally, matrix metalloproteinase-9 (MMP-9) was found to be strongly upregulated in uPAR-del4/5 overexpressing compared to vector control cells. uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells.


Corresponding author: Viktor Magdolen, Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Ismaninger Str. 22, D-81675 Munich, Germany

Received: 2012-5-16
Accepted: 2012-7-2
Published Online: 2012-11-13
Published in Print: 2012-12-01

©2012 by Walter de Gruyter Berlin Boston