Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter January 8, 2013

ADAMTS: Novel proteases expressed by activated mast cells

  • Gianni García-Faroldi EMAIL logo , Elin Rönnberg , Adolfo Orro , Gabriela Calounova , Bengt Guss , Anders Lundequist and Gunnar Pejler EMAIL logo
From the journal Biological Chemistry


Here we show that mast cells (MCs) express the metalloproteases of the Adisintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, and that ADAMTS expression is influenced by MC activation. Co-culture of MCs with live Gram-positive bacteria caused a profound induction of ADAMTS-9 and -6, as well as down-regulated expression of ADAMTS-5. Similar patterns were also seen after MC activation with calcium ionophore and by immunoglobulin E receptor crosslinking. Moreover, ADAMTS-5, -6 and -9 were all induced by activation of terminally differentiated murine peritoneal MCs and in a human MC line. ADAMTS-9 up-regulation in response to immunoglobulin E receptor crosslinking was strongly dependent on Gö6976-sensitive protein kinase C and partly dependent on nuclear factor of activated T cells and nuclear factor kappa-light-chain-enhancer of activated B cells, respectively. The expression of ADAMTS-5, -6 and -9 was closely linked to MC maturation, as shown by their strong induction during the differentiation of bone marrow precursor cells into mature MCs. ADAMTS family members have been shown to possess aggrecanase activity. Accordingly, MCs were shown to express aggrecanase activity. Finally, ADAMTS-5 protein was detected in MCs by immunocytochemistry. Taken together, the present study reveals ADAMTS expression by MCs and that MC activation regulates the expression of these proteases, thus implicating the ADAMTS family of proteases in MC function.

Corresponding authors: Gianni García-Faroldi and Gunnar Pejler, Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, Uppsala, Sweden

Received: 2012-8-13
Accepted: 2012-11-8
Published Online: 2013-01-08
Published in Print: 2013-02-01

©2013 by Walter de Gruyter Berlin Boston

Downloaded on 24.9.2023 from
Scroll to top button