Corrigendum to: Sangeeta Mehta, Rakhee Chhetra, Radhika Srinivasan, Suresh C. Sharma, Digambar Behera and Sujata Ghosh; Potential importance of Maackia amurensis agglutinin in non-small cell lung cancer. Biol. Chem., Volume 394, Issue 7, 2013, pages, 889–900 (DOI: 10.1515/hsz-2012-0279):
The concentration of lectins (Maackia amurensis agglutinin & Sambucus nigra agglutinin ) have been erroneously specified in units of µm whereas it should be nm. The corrected units in text and figure appears below.
Apoptosis induced by Maackia amurensis agglutinin (■) and Sambucus nigra agglutinin (□) in NCI-H460 cells. (A) and NCI-H520 cells, (B) at 24 h.
The apoptotic index was evaluated by CDD-ELISA. ***p<0.01 vs. cells treated with 0.8 nm of Maackia amurensis agglutinin; ++p<0.01 vs. cells treated with 4 nm of Sambucus nigra agglutinin; one way ANOVA Post Hoc Dunnett test. Representative histograms of FACS analysis of Maackia amurensis agglutinin treated (C) NCI-H460 cells and (D) NCI-H520 as evaluated by APO-Direct Kit. Cells only (a, a′), cells cultured in presence of 0.8 nm lectin (b, b′), 1.6 nm lectin (c, c′) and 4 nm lectin (d, d′); Inset: Graphical representation of the lectin-induced apoptosis in both the cell lines. **p<0.01 vs. untreated cells. One way ANOVA Post Hoc Dunnett test. Each bar represents mean±SD of values obtained from three independent experiments performed in duplicate.
[P 892]
The apoptotic index of NCI-H460 cells (Figure 3A) and NCI-H520 cells (Figure 3B) was at the maximum at a 4 nm dose of Macckia amurensis agglutinin, which was down- regulated at higher doses.
[P 892]
The percentage of apoptotic cells was significantly higher (p<0.01) in cells [47±5.1% for NCI-H460 and 54±5.2% for NCI-H520] cultured for 24 h in the presence of 4 nm lectin as compared to the respective control cells.
[P 893]
When both the cell lines were incubated for 12 h with the lectin (4 nm), the extent of apoptosis was found to be comparable to that of the respective control, while 24 h and 36 h incubation increased the apoptotic index to ~2.5-fold and ~4-fold, respectively. As 24 h of incubation of both the NSCLC cell lines with the 4 nm dose of Maackia amurensis agglutinin was sufficient for inducing an appreciable extent of apoptosis, this dose of the lectin for 24 h was used for the induction of apoptosis in both the cell lines (5×104/500 μl) in the subsequent experiments. The apoptotic index of normal lung fibroblast cells (WI-38) cultured for 24 h in presence of 4 nm dose of Maackia amurensis agglutinin was found to be comparable to that of the untreated cells.
[P 893]
We observed that pre-incubation of the lectin (4 nm) with GM2 (0.78 ng/ml) resulted in a decrease in the apoptotic index by 42% and 39% in NCI-H460 cells and NCI-H520 cells, respectively. The apoptotic index of NCI-H460 and NCI-H520 cells was found to be reduced by 45% and 46% respectively, when the cells were treated with the lectin (4 nm) pre-incubated with IgGMAA (Figure 4B).
[P 893]
Figure 4 (A) Apoptosis induced by Maackia amurensis agglutinin (4 nm) in NCI-H460 cells and NCI-H520 cells at various time periods.
[P 897]
Briefly, the NSCLC cells (5×104/500 μl) were cultured in serum-free media in the absence and presence of different doses (0.8–8 nm) of Macckia amurensis agglutinin for 24 h.
Briefly, the cells were cultured in the absence and presence of different doses (0.8–4 nm) of Maackia amurensis agglutinin for 24 h, washed and fi xed in 1% paraformaldehyde for 15 min at 4°C.
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