Accessible Unlicensed Requires Authentication Published by De Gruyter July 23, 2016

The power, pitfalls and potential of the nanodisc system for NMR-based studies

Aldino Viegas ORCID logo, Thibault Viennet and Manuel Etzkorn ORCID logo
From the journal Biological Chemistry

Abstract

The choice of a suitable membrane mimicking environment is of fundamental importance for the characterization of structure and function of membrane proteins. In this respect, usage of the lipid bilayer nanodisc technology provides a unique potential for nuclear magnetic resonance (NMR)-based studies. This review summarizes the recent advances in this field, focusing on (i) the strengths of the system, (ii) the bottlenecks that may be faced, and (iii) promising capabilities that may be explored in future studies.

Acknowledgments

The authors acknowledge access to the Jülich-Düsseldorf Biomolecular NMR Center, support by Dr. Fabien Aussenac (Bruker Biospin) and thank the DFG for support through the Emmy Noether grants ET103/2-1 to M.E. as well as the Marie Sklodowska-Curie Grant No. 660258 to A.V. T.V. acknowledges support from the International NRW Research School iGRASPseed.

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Received: 2016-6-6
Accepted: 2016-7-19
Published Online: 2016-7-23
Published in Print: 2016-12-1

©2016 Walter de Gruyter GmbH, Berlin/Boston