Abstract
ABC exporters are highly dynamic membrane proteins that span a huge spectrum of different conformations. A detailed integrated approach of cellular, biochemical and biophysical characterization of these ‘open’, ‘closed’ and other intermediate states is central to understanding their function. Almost 40 years after the discovery of the first ABC transporter, thanks to the enormous development in methodologies, a picture is slowly emerging to visualize how these fascinating molecules transport their substrates. This mini review summarizes some of the biophysical tools that have made a major impact in understanding the function of the ABC exporters.
Funding source: Deutsche Forschungsgemeinschaft
Award Identifier / Grant number: GZ:SR 113/1-1
Funding statement: We gratefully acknowledge the generous financial support of the Deutsche Forschungsgemeinschaft (DFG Research Grant) (Grant/Award Number: ‘GZ:SR 113/1-1’) and the LOEWE program of the State of Hesse.
Acknowledgements
We gratefully acknowledge the generous financial support of the Deutsche Forschungsgemeinschaft (DFG Research Grant) (Grant/Award Number: ‘GZ:SR 113/1-1’) and the LOEWE program of the State of Hesse.
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