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Licensed Unlicensed Requires Authentication Published by De Gruyter October 10, 2016

Biophysical methods toolbox to study ABC exporter structure and function

Thomas Marcellino and Vasundara Srinivasan EMAIL logo
From the journal Biological Chemistry

Abstract

ABC exporters are highly dynamic membrane proteins that span a huge spectrum of different conformations. A detailed integrated approach of cellular, biochemical and biophysical characterization of these ‘open’, ‘closed’ and other intermediate states is central to understanding their function. Almost 40 years after the discovery of the first ABC transporter, thanks to the enormous development in methodologies, a picture is slowly emerging to visualize how these fascinating molecules transport their substrates. This mini review summarizes some of the biophysical tools that have made a major impact in understanding the function of the ABC exporters.

Award Identifier / Grant number: GZ:SR 113/1-1

Funding statement: We gratefully acknowledge the generous financial support of the Deutsche Forschungsgemeinschaft (DFG Research Grant) (Grant/Award Number: ‘GZ:SR 113/1-1’) and the LOEWE program of the State of Hesse.

Acknowledgements

We gratefully acknowledge the generous financial support of the Deutsche Forschungsgemeinschaft (DFG Research Grant) (Grant/Award Number: ‘GZ:SR 113/1-1’) and the LOEWE program of the State of Hesse.

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Received: 2016-6-30
Accepted: 2016-10-5
Published Online: 2016-10-10
Published in Print: 2017-2-1

©2017 Walter de Gruyter GmbH, Berlin/Boston

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