Abstract
Treatment of different cell lines with progesterone receptor membrane component 1 (PGRMC1) antagonist AG-205 rapidly induces the formation of large vesicular structures that likely represent endosomes. Crispr/Cas9 was used to target the PGRMC1 and progesterone receptor membrane component 2 (PGRMC2) genes in CHO-K1 and HeLa. Unexpectedly, deficiency in one of these or both genes did not inhibit the formation of enlarged vesicles by AG-205, demonstrating additional molecular target(s) of this compound besides PGRMC1. Thus, AG-205 cannot be regarded as a PGRMC1-specific antagonist. However, provided that its currently unknown target(s) will be identified, AG-205 may serve as a new reagent to study endosomal trafficking.
Acknowledgements
We thank Ivonne Becker for expert technical assistance and Simone Diestel for kindly providing several plasmids used in this study. This study was supported by grants from the Deutsche Forschungsgemeinschaft to M. E.
Funding: Deutsche Forschungsgemeinschaft, Funder Id: http://dx.doi.org/10.13039/501100001659, Grant Number: Sonderforschungsbereich SFB645 Project B5.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2019-0417).
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