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Licensed Unlicensed Requires Authentication Published by De Gruyter January 19, 2012

A modified simple method for determination of serum α-tocopherol (vitamin E)

  • Jameel G. Jargar , Shaheenkousar H. Hattiwale , Swastika Das , Salim A. Dhundasi and Kusal K. Das EMAIL logo
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Background: Vitamin E is one of the important antioxidants linked to regulate various diseases, such as atherosclerosis, hypertension, and male infertility. A relatively simple and economic biochemical modified method has been developed to determine serum α-tocopherol concentration.

Methods: The current modified method is based on previous Baker and Frank method and the method of Martinek by using 2,2′-bipyridyl, ferric chloride, and xylene. The complex of ferrous ions generated in this reaction with 2,2′-bipyridyl is determined by using a plain enzyme-linked immunosorbent assay microplate (non-antibody coated) at 492 nm.

Results: The standard curve of this new modified method shows a linearity with correlation r=0.997 (concentration vs. absorbance). The absorbance of this color complex is directly proportional to the α-tocopherol concentration. The sensitivity of this new modified method has been compared and correlated with Baker and Frank method by using 15 human samples (r=0.99, p<0.0001).

Conclusions: This simple and economic method may be routinely used to analyze α-tocopherol concentration in serum.

Corresponding author: Prof. Kusal K. Das, Department of Physiology, Environmental Health Research Unit, Al-Ameen Medical College, Bijapur-586108, Karnataka, India Phone: (W) +91-8352-272502, +91-8352-271257, Fax: +91-8352-270184

Received: 2011-10-14
Accepted: 2011-12-10
Published Online: 2012-01-19
Published in Print: 2012-03-01

©2012 by Walter de Gruyter Berlin Boston

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