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Licensed Unlicensed Requires Authentication Published by De Gruyter November 28, 2013

Anti-inflammatory and analgesic effects of methanol extract and fractions of Acalypha wilkesiana leaves

  • Johnny O. Olukunle EMAIL logo , Olubukola T. Adenubi , Khalid T. Biobaku and Emmanuella A.O. Sogebi


Background: This study was carried out to evaluate the anti-inflammatory and the analgesic potentials of Acalypha wilkesiana leaf extract in experimental animals.

Methods: The anti-inflammatory potential was investigated using an acute rat model. Aspirin at 150 mg/kg, indomethacin at 10 mg/kg as positive control, and methanolic extracts and solvent fractions of A. wilkesiana at doses of 400 and 200 mg/kg, respectively, were administered orally to rats and compared with a negative control group given 10 mL/kg Tween 80. At an A. wilkesiana dose of 400 mg/kg body weight, the analgesic effect was also studied using the hot plate method and the acetic acid-induced writhing model in mice.

Results: Percentage inhibition of the paw volume was highest in rats administered indomethacin (85.7%) followed by the group administered methanolic extract of A. wilkesiana (74.1%), whereas the group given aspirin had 65.5% inhibition of edema and the group given a 200 mg/kg dose of chloroform fraction of A. wilkesiana had 93% inhibition of increase in paw volume, a value higher than the performance of the standard drugs indomethacin (85%) and aspirin (68%). Also, the extract caused an increase in the reaction time in the hot plate test and in the acetic acid test. The mean number of writhings was significantly reduced in the group of mice administered 400 mg/kg extract of A. wilkesiana leaves (25.8±1.3) when compared with the control (46.7±1.4) but higher than the group of mice administered the standard analgesic agent paracetamol (23.5±1.3).

Conclusions: This study suggested that A. wilkesiana leaves have anti-inflammatory and analgesic potential comparable with those of standard drugs.

Corresponding author: Johnny O. Olukunle, Department of Veterinary Physiology and Pharmacology, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun State, Nigeria, Phone: +234 810 184 6078, E-mail:


1. Cotran RS, Kumar V, Collins T. Robbins pathologic basis of disease, 6th ed. Philadelphia, PA: W.B. Saunders, 2001:51.Search in Google Scholar

2. Ogunyemi MA: The origin of the herbal cure and its spread. University of Ife Press, Ile-Ife, 1979:20–2.Search in Google Scholar

3. Kokko JP. Site and mechanism of action of diuretics. Am J Med 1984;77:11–7.10.1016/S0002-9343(84)80003-0Search in Google Scholar

4. Oluyemi A. Bridella ferrugenea. Medicinal plants and their therapeutic uses in South-Western zone of Nigeria. Technical report, Nigeria, University Press, 1998:101.Search in Google Scholar

5. Olukunle JO, Abatan MO, Adenubi OT, Oyebanji OO, Ogbole OO. Anti-inflammatory and anti-nociceptive effects of extract and fractions of Morinda morindoides root. Int Res J Pharm Pharmacol 2012;2:118–25.Search in Google Scholar

6. Olukunle JO, Adenubi OT, Sogebi EA, Oguntoke PC. Analgesic and anti-inflammatory potential of Jatropha carcus. Acta Vet Brno 2011;80:259–62.10.2754/avb201180030259Search in Google Scholar

7. Hikino HK, Ogata YK, Konno C. Pharmacology of ephedroxanes. J Ethnopharmacol 1985;13:175–91.10.1016/0378-8741(85)90005-4Search in Google Scholar

8. Kiuchi F, Iwakami S, Shibuya M, Hanaoka F, Sankawa U. Inhibition of prostaglandin and leucotriene biosynthesis by gingerols and diarylheptanoids. Chem Pharm Bull 1992;40:387–91.10.1248/cpb.40.387Search in Google Scholar

9. Fernandez E, Boyle GJ. Affective and evaluative descriptors of pain in the McGill Pain Questionnaire: reduction and reorganization. J Pain. 2001;2:318–25. Erratum in J Pain. 2002;3:69. Corrected and republished in J Pain 2002;3:70–7.Search in Google Scholar

10. Millan MJ. Serotonin and pain: evidence that activation of 5-HT1A receptors does not elicit antinociception against noxiothermal, mechanical and chemical stimuli in mice. Pain 1994;58:45–61.10.1016/0304-3959(94)90184-8Search in Google Scholar

11. Reisine T, Pasternak G. Opioid analgesics and antagonists. In: Hardman JG, Limbird LE, Molinoff PB, Ruddon RW, Gilman AG, editors. Goodman and Gilman’s The Pharmacological Basis of Therapeutics, 9th ed. New York: McGraw Hill, 1996:521–55.Search in Google Scholar

12. Do Monte FH, dos Santos JG, Russi M, Lanziotti VM, Leal LK, Cunha GM. Antinociceptive and anti-inflammatory properties of the hydroalcoholic extract of stems from Equisetum arvense L. in mice. Pharmacol Res 2004;49:239–43.10.1016/j.phrs.2003.10.002Search in Google Scholar PubMed

Received: 2013-6-9
Accepted: 2013-10-22
Published Online: 2013-11-28
Published in Print: 2015-3-1

©2013 by De Gruyter

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