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Licensed Unlicensed Requires Authentication Published by De Gruyter April 21, 2014

Gastroprotective activity of essential oils from turmeric and ginger

  • Vijayasteltar B. Liju , Kottarapat Jeena and Ramadasan Kuttan EMAIL logo

Abstract

Background: Turmeric (Curcuma longa) and ginger (Zingiber officianale) are widely used in Asian countries as traditional medicine and food ingredients. In the present study, we have evaluated the gastroprotective activity of turmeric essential oil (TEO) and ginger essential oil (GEO) in rats.

Methods: Turmeric and ginger were evaluated for their antiulcer activity against ethanol-induced ulcers in male Wistar rats at different doses: 100, 500 and 1000 mg/kg body weight. Ethanol was used to induce gastric ulcer in Wistar rats. Parameters such as ulcer index, histopathology and levels of antioxidant enzymes such as glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase and glutathione (GSH) levels were measured to assess the degree of protection produced by the essential oils.

Results: TEO and GEO inhibited ulcer by 84.7% and 85.1%, respectively, as seen from the ulcer index. Reduced antioxidant enzymes such as GPx, SOD, catalase and GSH produced by alcohol administration were significantly (p<0.001) increased by simultaneous administration of TEO and GEO. Histopathological examination showed that ethanol-induced lesions such as necrosis, erosion and hemorrhage of the stomach wall were significantly reduced after oral administration of essential oils.

Conclusions: Results suggest that TEO and GEO could reduce the gastric ulcer in rat stomach as seen from the ulcer index and histopathology of the stomach. Moreover, oxidative stress produced by ethanol was found to be significantly reduced by TEO and GEO.


Corresponding author: Dr. Ramadasan Kuttan, PhD, Research Director, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala-680 555, India, Phone: +91-487-2307968, Fax: +91-487-2307968, E-mail: ;

Acknowledgments

The authors thank Spices Board, Cochin, India for providing funds (grant no. MD/M&H/01/2008-09) to carry out this work.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research funding: The financial support for this work was provided by Spices Board, Cochin, India.

Employment or leadership: None declared.

Honorarium: None declared.

References

1. Buchbauer G. The detailed analysis of essential oils leads the understanding of their properties. Perfum Flavor 2000;25:64–7.Search in Google Scholar

2. Zu Y, Yu H, Liang L, Fu Y, Efferth T, Liu X, et al. Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules 2010;15:3200–10.10.3390/molecules15053200Search in Google Scholar PubMed PubMed Central

3. Liju VB, Jeena K, Kuttan R. An evaluation of antioxidant, anti-inflammatory, and antinociceptive activities of essential oil from Curcuma longa L. Indian J Pharmacol 2011;43:526–31.10.4103/0253-7613.84961Search in Google Scholar PubMed PubMed Central

4. Jeena K, Liju VB, Kuttan R. Antioxidant, anti-inflammatory and antinociceptive activities of essential oil from ginger. Indian J Physiol Pharmacol 2013;57:51–62.Search in Google Scholar

5. Rathore P, Dohane P, Varma S, Ray A, Sharma U, Jagannathan MR, et al. Curcuma oil: reduces early accumulation of oxidative product and is antiapoptogenic in transient focal ischemia in rat brain. Neurochem Res 2008;33:1672–82.10.1007/s11064-007-9515-6Search in Google Scholar PubMed

6. Dohare P, Garg P, Sharma U, Jagannathan NR, Ray M. Neuroprotective efficacy and therapeutic window of curcuma oil: in rat embolic stroke model. BMC Complement Altern Med 2008;8:55.10.1186/1472-6882-8-55Search in Google Scholar PubMed PubMed Central

7. Deepa DA, Anita B, Sreelatha KT. Comparative study of the efficacy of curcumin and turmeric oil as chemopreventive agents in oral submucous fibrosis: a clinical and histopathological evaluation. JIAOMR 2010;22:88–92.10.5005/jp-journals-10011-1021Search in Google Scholar

8. Jayaprakasha JK, Jena BS, Negi PS, Sakariah KK. Evaluation of antioxidant activities and antimutagenicity of turmeric oil: a byproduct from curcumin production. Z Naturforsch C 2002;57:828–35.10.1515/znc-2002-9-1013Search in Google Scholar PubMed

9. Park SY, Kim YH, Lee SJ. Aromatic-turmerone attenuates invasion and expression of MMP-9 and COX-2 through inhibition of NF-kB activation in TPA-induced breast cancer cells. J Cell Biochem 2012;113:3653–62.10.1002/jcb.24238Search in Google Scholar PubMed

10. Zhou HI, Deng M, Xie QM. The modulatory effects of the volatile oils of ginger on the cellular immune response in vitro and in vivo in mice. J Ethnopharmacol 2006;105:301–5.10.1016/j.jep.2005.10.022Search in Google Scholar PubMed

11. Jeena K, Liju VB, Kuttan R. Ginger essential oil inhibits chemically induced carcinogenesis in animal models. J Ethnobiol Tradit Med 2014;122:832–41.Search in Google Scholar

12. Hashim S, Aboobaker VS, Madhubala R, Bhattacharya RK, Rao AR. Modulatory effects of essential oils from spices on the formation of DNA adduct by aflatoxin B1 in vitro. Nutr Cancer 1994;21:169–75.10.1080/01635589409514314Search in Google Scholar PubMed

13. Liju VB, Jeean K, Kuttan R. Acute and subchronic toxicity as well as mutagenic evaluation of essential oil from turmeric (Curcuma longa L). Food Chem Toxicol 2013;53:52–61.10.1016/j.fct.2012.11.027Search in Google Scholar PubMed

14. Jeena K, Liju VB, Kuttan R. Apreliminary 13 weeks oral toxicity study of ginger oil in male and female Wistar rats. Int J Toxicol 2011;23:662–70.10.1177/1091581811419023Search in Google Scholar

15. Kalra P, Sharma S, Suman, Kumar S. Antiulcer effect of the methanolic extract of tamarindus indica seeds in different experimental models. J Pharm Bioallied Sci 2011;3:236–41.10.4103/0975-7406.80778Search in Google Scholar

16. Hoogerwerf WA, Pasricha PJ. Agents used for the control of gastric acidity and treatment of peptic ulcers and gastrooesophageal reflux diseases. In: Hardman JG, Limbri LE, Goodman CS, Gilman BT, editors. The pharmacological basis of therapeutics, 10th ed. New York: McGraw-Hill, 2001:11005–10.Search in Google Scholar

17. Hafeman DG, Sunde RA, Hoekstra WG. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr 1974;104:580–7.10.1093/jn/104.5.580Search in Google Scholar

18. Belaiche J, Burette A, De Vos M, Louis E, Huybrechts M, Deltenre M. Study Group of NSAID-GI Complications. Observational survey of NSAID-related upper gastro-intestinal adverse events in Belgium. Acta Gastroenterol Belg 2002;65:65–73.Search in Google Scholar

19. Holzer P, Livingston EH, Saria A, Guth PH. Sensory neurons mediate protective vasodilatation in rat gastric mucosa. Am J Physiol 1991;260:363–70.10.1152/ajpgi.1991.260.3.G363Search in Google Scholar

20. Ketuly MA, Abdulla KA, Hadi HA, Mariod AA, Abdel-Wahab SI. Antiulcer activity of 9alpha bromo analogue of Beclomethasonedipropionate against ethanol induced gastric mucosal injury in rats. J Med Plants Res 2011;5:514–20.Search in Google Scholar

21. Takagi K, Okabe S, Saziki R. A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing. Jpn J Pharmacol 1969;19:418–26.10.1254/jjp.19.418Search in Google Scholar

22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem 1951;19:265–75.10.1016/S0021-9258(19)52451-6Search in Google Scholar

23. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat liver. Biochim Biophys Acta 1979;582:67–78.10.1016/0304-4165(79)90289-7Search in Google Scholar

24. Aebi H. Catalase estimation. In: Bergmeyer HU, editor. Methods of enzymatic analysis. New York: Verlag Chemie, 1974:673–84.Search in Google Scholar

25. McCord JK, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969;244:6049–55.Search in Google Scholar

26. Bharathi DP, Jagadeesan M. Gastro protective activity of Momordica cymbalaria fruits against experimentally induced gastric ulcer in rats. Int J Phytomed 2010;2:385–9.Search in Google Scholar

27. Hiruma-Lima CA, Batista LM, de Almeida AB, de Pietro Magri L, dos Santos LC, Vilegas W, et al. Antiulcerogenic action of ethanolic extract of the resin from Virola surinamensis Warb. (Myristicaceae). J Ethnopharmacol 2009;122:406–9.10.1016/j.jep.2008.12.023Search in Google Scholar

28. Massignan JJ, Lemos M, Maistro EL, Schaphauser HP, Jorge RF, Sousa JP, et al. Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on different experimental models in rats. Phytother Res 2009;23:1355–60.10.1002/ptr.2624Search in Google Scholar

29. Mizui T, Sato H, Hirose F, Doteuchi M. Effect of antiperoxidative drugs on gastric damage induced by ethanol in rats. Life Sci 1987;41:755–63.10.1016/0024-3205(87)90456-5Search in Google Scholar

30. Oates PJ, Hakkinen JP. Studies on the mechanism of ethanol induced gastric damage in rats. Gastroenterology 1988;94:10–21.10.1016/0016-5085(88)90604-XSearch in Google Scholar

31. Pozzato G, Franzin F, Moretti M, Lachin L, Benedetti G, Sablich R, et al. Effects of omeprazole on ethanol metabolism: an in vitro and in vivo rat and human study. Pharmacol Res 1994;29:47–58.10.1016/1043-6618(94)80097-9Search in Google Scholar

32. Jeena K, Liju VB, Kuttan R. Antimutagenic potential and modulation of carcinogen-metabolizing enzymes by ginger essential oil. Phytother Res 2013; Epub ahead of print 10 Sep 2013. DOI: 10.1002/ptr.5064.10.1002/ptr.5064Search in Google Scholar

33. Demir S, Yilmaz M, Koseoglu M, Akalin N, Aslan D, Aydin A. Role of free radicals in peptic ulcer and gastritis. Turk J Gastroenterol 2003;14:39–43.Search in Google Scholar

34. Wakulich CA, Tepperman BL. Role of glutathione in nitric oxide mediated injury to rat gastric mucosal cells. Eur J Pharmacol 1997;319:333–41.10.1016/S0014-2999(96)00865-5Search in Google Scholar

35. Loguercio C, Taranto D, Beneduce F, del Vecchio Blanco C, de Vincentis A, Nardi G, et al. Glutathione prevents ethanol-induced gastric mucosal damage and depletion of sulfhydryl compounds in humans. Gut 1993;34:161–5.10.1136/gut.34.2.161Search in Google Scholar PubMed PubMed Central

36. Bannister J, Bannister W. Aspects of the structure, function and application of superoxide dismutase. CRC Crit Rev Biochem 1987;22:111–8.10.3109/10409238709083738Search in Google Scholar PubMed

37. Prakash P, Misra A, Surin WR, Jain M, Bhatta RS, Pal R, et al. Anti-platelet effects of Curcuma oil in experimental models of myocardial ischemia-reperfusion and thrombosis. Thromb Res 2011;127:111–8.10.1016/j.thromres.2010.11.007Search in Google Scholar PubMed

Received: 2013-12-20
Accepted: 2014-3-5
Published Online: 2014-4-21
Published in Print: 2015-1-1

©2015 by De Gruyter

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