Background: Considering the antihyperglycemic and antioxidant activities of herbs, this study has evaluated the morphological changes, hypoglycemic effect, and comparative ameliorating effects of Costus afer Ker Gawl leaf and glibenclamide on pancreatic injury induced by alloxan.
Methods: Thirty adult male albino rats that were divided into six groups of five weight-matched animals each were used in the study. Groups 1 and 2 served as controls, whereas groups 3–6 were alloxan-induced diabetic groups treated with different doses of the extract (375, 750, and 1125 mg/kg C. afer) and glibenclamide, respectively. The glucose level was measured daily, whereas the weight of the animal was monitored on a weekly basis for 21 days. The oral glucose tolerance test was measured on overnight fasted rats after glucose load at 0, 30, 60, 90, and 120 min. The histopathology of the pancreas was also investigated.
Results: The phytoconstituents of C. afer Ker Gawl leaves include glycosides, tannins, saponins, terpenoid, phenolic compound, flavonoids, and alkaloids. Costus afer possessed significant hypoglycemic (p<0.05) effect and reversed the histopathologic damage of pancreases in alloxan-induced diabetic rats comparable to those of glibenclamide.
Conclusions: Costus afer leaves possess both antidiabetic and tissue protective properties on pancreases of investigated rats.
We acknowledge Mr. A.O. Ozioko (INTERCEDD, University of Nigeria, Nsukka, Enugu State, Nigeria) and Professor M.E. Bassey (Botany Department, University of Uyo, Akwa Ibom State, Nigeria) for the identification and authentication of the plant.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
2. Scaglia L, Smith FE, Bonner-Weir S. Apoptosis contribute to the involution of β-cell mass in the postpartum rat pancreas. Endocrinology 1995;136:5461–8.10.1210/endo.136.12.7588296Search in Google Scholar PubMed
3. Adeyemi DO, Komolafe OA, Adewole SO, Obuotor EM, Adenowo TK. Effects of Annona muricata (Linn) on the morphology of pancreatic islet cells of experimentally-induced diabetic Wistar rats. Internet J Altern Med 2008;2:5.Search in Google Scholar
4. Meghana K, Sanjeev G, Ramesh B. Curcumin prevents streptozotocin induced islet damage by scavenging free radicals: a prophylactic and protective role. Eur J Pharmacol 2007;577:183–91.10.1016/j.ejphar.2007.09.002Search in Google Scholar PubMed
5. Anwannil HG, Atta R. Trends in ethnopharmacology. J Ethnopharmacol 2006;100:43–9.Search in Google Scholar
8. Schmelzer GH, Gurib-Fakim A. Plant resources of tropical Africa. In: Med. Plants 2008;1:191–4.Search in Google Scholar
10. Ezejiofor AN, Orish CN, Orisakwe OE. Effect of aqueous leaves extract of Costus afer Ker Gawl (Zingiberaceae) on the liver and kidney of male albino Wistar rat. Ancient Sci Life 2013;33:4–9.10.4103/0257-7941.134554Search in Google Scholar PubMed PubMed Central
11. Sofowara A. Medical plants and traditional medicine in Africa, Rep. Edition. Ibadan: Spectrum Books Ltd., 2006:150pp.Search in Google Scholar
12. N’guessan K, Amoikon KE, Soro D. Effect of aqueous extract of Persea americana seeds on the glycemia of diabetic rabbits. Eur J Sci Res 2009;26:376–85.Search in Google Scholar
13. Jamshidzadeh A, Baghban M, Azarpira N, Mohammadi Bardbori A, Niknahad H. Effects of tomato extract on oxidative stress induced toxicity in different organs of rats. Food Chem Toxicol 2008;46:3612–5.10.1016/j.fct.2008.09.006Search in Google Scholar PubMed
14. Sasidharan S, Sumathi V, Jegathambigai NR, Latha LY. Anti-hyperglycaemic effects of ethanol extracts of Carica papaya and Pandanus amaryfollius leaf in streptozotocin-induced diabetic mice. Nat Prod Res 2011;25:982–7.10.1080/14786419.2010.523703Search in Google Scholar PubMed
15. Juárez-Rojop IE, Díaz-Zagoya JC, Ble-Castillo JL, Miranda-Osorio PH, Castell-Rodríguez AE, Tovilla-Zárate CA, et al. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats. BMC Complement Altern Med 2012;12:236.10.1186/1472-6882-12-236Search in Google Scholar PubMed PubMed Central
16. Gupta R, Sharma AK, Sharma MC, Gupta RS. Antioxidant activity and protection of pancreatic β-cells by embelin in streptozotocin-induced diabetes. J Diabetes 2012;4:248–56.10.1111/j.1753-0407.2012.00187.xSearch in Google Scholar
17. Adisa RA, Choudhary MI, Olorunsogo OO. Hypoglycemic activity of Buchholzia coriacea (Capparaceae) seeds in streptozotocin-induced diabetic rats and mice. Exp Toxicol Pathol 2011;63:619–25.10.1016/j.etp.2010.05.002Search in Google Scholar
18. Bera TK, De D, Chatterjee K, Ali KM, Ghosh D. Effect of Diashis, a polyherbal formulation, in streptozotocin-induced diabetic male albino rats. Int J Ayurveda Res 2010;1:18–24.10.4103/0974-7788.59939Search in Google Scholar
19. Abeywickrama KR, Ratnasooriya WD, Amarakoon AM. Oral hypoglycaemic, antihyperglycaemic and antidiabetic activities of Sri Lankan broken orange pekoe fannings (BOPF) grade black tea (Camellia sinensis L.) in rats. J Ethnopharmacol 2011;135:278–86.10.1016/j.jep.2011.02.035Search in Google Scholar
20. Pepato MT, Baviera AM, Vendramini RC, Brunetti IL. Evaluation of toxicity after one-month treatment with Bauhinia forficata decoction in streptozotocin-induced diabetic rats. BMC Complement Altern Med 2004;8:4–7.10.1186/1472-6882-4-7Search in Google Scholar
21. Chaiyasut C, Kusirisin W, Lailerd N, Lerttrakarnnon P, Suttajit M, Srichairatanakool S. Effects of phenolic compounds of fermented Thai indigenous plants on oxidative stress in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med 2011;13:10.10.1155/2011/749307Search in Google Scholar
22. Anyasor GN, Ogunwenmo KO, Olatunji AO, Blessing EA. Phytochemical, proximate and mineral element composition of stem of Costus afer (Bush cane). Asian J Plant Sci Res 2010;2:607–12.Search in Google Scholar
23. Suganya S, Narmadha VK, Gopalakrishnan KD. Hypoglycemic effect of Costus pictus D. Don on alloxan induced type 2 diabetic mellitus in albino rats. Asian Pac J Trop Dis 2012;117–23.10.1016/S2222-1808(12)60028-0Search in Google Scholar
24. Lukaariova A, Mojzis J, Benacka R, Keller J, Maguth T, Kurila P, et al. Preventive effect of flavonoids on alloxan induced diabetes mellitus in rats. Acta Vet Brno 2008;77:175–82.10.2754/avb200877020175Search in Google Scholar
25. Nakashima N, Kimura I, Kimura M, Matsura H. Isolation of pseudoprototimosaponin AIII from rhizomes of Anemarrhena asphodeloides and its hypoglycemic activity in streptozotocin-induced diabetic mice. J Nat Prod 1993;56:345–50.10.1021/np50093a006Search in Google Scholar PubMed
26. Waer HF, Helmy SA. Cytological and histochemical studies in rat liver and pancreas during progression of streptozotocin induced diabetes and possible protection of certain natural antioxidants. J Nutr Food Sci 2012;2:165.Search in Google Scholar
27. Ramadan G, Nadia M, Beih E, Ema A, Ghffar AE. Modulatory effect of black vs. green aqueous extract hyperglycaemia, hyperlipidemia and liver dysfunction in diabetic and obese rat models. Br J Nutr 2009;102:1611–9.10.1017/S000711450999208XSearch in Google Scholar PubMed
28. Nasri S, Roghani M, Baluchnejadmojarad T, Balvardi M, Rabani T. Chronic cyanidin-3-glucoside administration improves short-term spatial recognition memory but not passive avoidance learning and memory in streptozotocin-diabetic rats. Phytother Res 2012;26:1205–10.10.1002/ptr.3702Search in Google Scholar PubMed
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