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Licensed Unlicensed Requires Authentication Published by De Gruyter May 28, 2015

Jobelyn® exhibited anti-inflammatory, antioxidant, and membrane-stabilizing activities in experimental models

  • Solomon Umukoro EMAIL logo , Oluwafemi Gabriel Oluwole , Anthony T. Eduviere , Omogbiya Itievere Adrian and Abayomi M. Ajayi

Abstract

Background: Jobelyn® (JB) is an African sorghum-based food supplement claimed to be efficacious for the treatment of rheumatoid arthritis (RA). Although in vitro studies confirmed its anti-inflammatory property, no study had shown the effect of JB using in vivo animal models of inflammation. Thus, its effects on acute and chronic inflammation in rats were evaluated in this study. Its effect on rat red blood cell (RBC) lysis was also assessed.

Methods: Acute inflammation was induced with intraplanter injection of carrageenan and increase in rat paw volume was measured using plethysmometer. The volume of fluid exudates, number of leukocytes, concentrations of malondialdehyde (MDA), and glutathione (GSH) in the fluid were measured on day 5 after induction of chronic inflammation with carrageenan in the granuloma air pouch model. RBC lysis induced by hypotonic medium as determined by release of hemoglobin was measured spectrophotometerically.

Results: JB (50–200 mg/kg) given orally produced a significant inhibition of acute inflammation induced by carrageenan in rats. It reduced the volume and number of leukocytes in inflammatory fluid in the granuloma air pouch model of chronic inflammation. It further decreased the levels of MDA in the fluid suggesting antioxidant property. JB elevated the concentrations of GSH in inflammatory exudates indicating free radical scavenging activity. It also significantly inhibited RBC lysis caused by hypotonic medium, suggesting membrane-stabilizing property.

Conclusions: JB has in vivo anti-inflammatory activity, which may be related to its antioxidant and membrane-stabilizing properties, supporting its use for the treatment of arthritic disorder.


Corresponding author: Solomon Umukoro, College Medicine, Department of Pharmacology and Therapeutics, University of Ibadan, Nigeria, Phone: +2348130897439, E-mail:

Acknowledgments

The authors appreciate the generous donation of samples of Jobelyn® by Mr. Olajuwon Okubena, the Director of Health Forever Products Inc., Lagos, Nigeria.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2014-10-16
Accepted: 2015-4-3
Published Online: 2015-5-28
Published in Print: 2015-9-1

©2015 by De Gruyter

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