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Accessible Unlicensed Requires Authentication Published by De Gruyter July 2, 2016

Antithrombotic and cytotoxic activities of four Bangladeshi plants and PASS prediction of their isolated compounds

Mohammad Shah Hafez Kabir, Md. Sofi Mahamoud, Nishan Chakrabarty, Shabbir Ahmad, Md. Abdullah Al Masum, Md. Akramul Hoque, Mohammed Munawar Hossain, Md. Mominur Rahman and Mir Muhammad Nasir Uddin

Abstract

Background:

This study aims to investigate whether tested organic extracts possess antithrombotic properties with minimal or no toxicity and to predict the activity of some of their isolated compounds.

Methods:

An in vitro thrombolytic model was used to check the clot lysis effect of four Bangladeshi herbal extracts viz., roots of Curculigo recurvata W.T. Aiton (Satipata), leaf of Amorphophallus bulbifer Roxb. (Olkachu), leaf of Phyllanthus sikkimensis Muell. Arg., and whole plant of Thunbergia grandiflora Roxb. (Nillata) using streptokinase as a positive control and water as a negative control. Cytotoxicity was screened by brine shrimp lethality bioassay using vincristine sulfate as positive control. In silico prediction of activity spectra for substances (PASS) prediction was applied for phytoconstituents, namely, nyasicoside, glucomannan, grandifloric acid, serine, and alanine.

Results:

Using an in vitro thrombolytic model, C. recurvata, A. bulbifer, P. sikkimensis, and T. grandiflora showed 28.10±1.64%, 42.47±1.96%, 32.86±1.92%, and 25.51±1.67% of clot lysis, respectively. Reference drug streptokinase exhibited 75.00±3.04% clot lysis. Examined herbs showed significant (p<0.001) percentage (%) of clot lysis compared to negative control. In brine shrimp cytotoxic assay, C. recurvata, A. bulbifer, P. sikkimensis, and T. grandiflora showed LC50 values 210.64±3.44, 98.51±1.47, 187.29±2.01, and 386.43±3.02 μg/mL, respectively, with reference to vincristine sulfate (LC50 0.76±0.04). PASS predicted that examined phytoconstituents have a wide range of biological activity.

Conclusions:

Through our study it was found that A. bulbifer and P. sikkimensis could be considered as very promising and beneficial thrombolytic agents.

Acknowledgments

The authors are grateful to the authority of International Islamic University Chittagong, Bangladesh, for providing the facilities to conduct this research work. The authors are thankful to all members of GUSTO (a research group) for their kind help in the experiment. The authors are also thankful to the Taxonomist and Professor, Dr. Shaikh Bokhtear Uddin, Department of Botany, University of Chittagong, for identifying all the plants.

  1. Author contributions: All the authors have accepted responsibility for the entire contents of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2015-11-24
Accepted: 2016-5-6
Published Online: 2016-7-2
Published in Print: 2016-11-1

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