Abstract
Background:
Persicaria orientalis, an ornamental medicinal plant, has been used in traditional medicine for the treatment of various diseases. Although the plant is reported to have some important pharmacological effects, many medicinal values remain unidentified. Our objective was to evaluate the anti-inflammatory, anti-diarrheal, thrombolytic, and cytotoxic properties of the methanol extract of P. orientalis leaves (Po-MeOH).
Methods:
Anti-inflammatory activity was measured by the inhibition of hypotonicity-induced human red blood cell hemolysis and albumin denaturation technique in vitro of Po-MeOH. Diarrheal episodes were examined in mice with castor oil-induced diarrhea. The clot lysis and brine shrimp lethality bioassay in vitro were used to evaluate the thrombolytic and cytotoxic activities of the plant extract, respectively.
Results:
Using in vitro anti-inflammatory models, the results demonstrated that Po-MeOH at the five different dose ranges from 31.25 to 500 μg/mL significantly (p<0.05) protected (0.98%–50.71%) the erythrocyte membrane against lysis induced by hypotonic medium solution and protein denaturation (38.27%–79.22%) of bovine albumin, respectively. The extract exhibited a significant reduction of severity (75.17%) of castor oil-induced diarrhea in mice at the highest dose of 400 mg/kg compared to loperamide (82.06%) at 5 mg/kg. Po-MeOH also showed 33.14% clot lytic activity in the thrombolytic test and cytotoxicity with LC50 value 58.91 μg/mL in the brine shrimp bioassay.
Conclusions:
These findings suggest that Po-MeOH has significant anti-inflammatory and anti-diarrheal effects along with moderate thrombolytic and lower cytotoxic properties that may warrant the further exploration.
Acknowledgments
We are grateful to the Department of Pharmacy, International Islamic University Chittagong, Bangladesh, for providing facilities for this research work.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. MJU and PA conceived and designed the experiments. PA, MJU, and MA carried out the anti-inflammatory and anti-diarrheal tests. MRI, MHA, and AR conducted the thrombolytic and cytotoxicity experiments. Under the supervision of MJU wrote the manuscript. MJU and MMR performed statistical analysis. All authors read and approved the final manuscript.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Rowman RM. The blood and bone marrow. In: MacSween RM, Whaley K, editors. Muir’s textbook of pathology, 13th ed. India: Thomas Press Ltd., 1966;585–644.Search in Google Scholar
2. Chou CT. The anti-inflammatory effect of Tripterygium wilfordii Hook F on adjuvant-induced paw edema in rats and inflammatory mediator’s release. Phytother Res 1997;11:152–4.10.1002/(SICI)1099-1573(199703)11:2<152::AID-PTR45>3.0.CO;2-LSearch in Google Scholar
3. Okoli CO, Akah PA, Onuoha NJ, Okoye TC, Nwoye AC, Nworu CS. Acanthus montanus: an experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Complement Altern Med 2008;8:27.10.1186/1472-6882-8-27Search in Google Scholar
4. Brijesh S, Daswani P, Tetali P, Anita N, Birdi T. Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: validating its traditional usage. BMC Complement Altern Med 2009;9:47.10.1186/1472-6882-9-47Search in Google Scholar
5. Lopez AD, Mathers CD. Measuring the global burden of disease and epidemiological transitions: 2002–2030. Ann Trop Med Parasitol 2006;100:481–99.10.1179/136485906X97417Search in Google Scholar
6. Briend A. Is diarrhea a major cause of malnutrition among the under-fives in developing countries? A review of available evidence. Eur J Clin Nutr 1990;44:611–28.Search in Google Scholar
7. Rahman, MA, Sultana R, Emran TB, Islam MS, Rahman MA, Chakma JS, et al. Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC Complement Altern Med 2013;13:25.10.1186/1472-6882-13-25Search in Google Scholar
8. Naderi GA, Asgary S, Jafarian A, Askari N, Behagh A, Aghdam RH. Fibrinolytic effects of Ginkgo biloba extract. Exp Clin Cardiol 2005;10:85.Search in Google Scholar
9. Semwal DK, Badoni R, Semwal R, Kothiyal SK, Singh GJ, Rawat U. The genus Stephania (Menispermaceae): chemical and pharmacological perspectives. J Ethnopharmacol 2010;132:369–83.10.1016/j.jep.2010.08.047Search in Google Scholar
10. Rahmatullah M, Ishika T, Rahman M, Swarna A, Khan T, Monalisa MN, et al. Plants prescribed for both preventive and therapeutic purposes by the traditional healers of the Bede community residing by the Turag river, Dhaka district. Am Eurasian J Sustain Agric 2011;5:325–31.Search in Google Scholar
11. Rahmatullah M, Khatun A, Morshed N, Neogi PK, Khan SU, Hossan S, et al. A randomized survey of medicinal plants used by folk medicinal healers of Sylhet division, Bangladesh. Adv Nat Appl Sci 2010;4:52–62.Search in Google Scholar
12. Medicinal plants of Bangladesh. Available at: http://www.mpbd.info/plants/persicaria-orientalis.php. Accessed 5 July 2015.Search in Google Scholar
13. Li YJ, He X, Liu LN, Lan YY, Wang AM, Wang YL. Studies on chemical constituents in herb of Polygonum orientale. China J Chin Mater Med 2005;30:444–6.Search in Google Scholar
14. Zheng S, Wang D, Meng J, Shen X. Studies on the lignans of Polygonum orientale. Acta Bot Sin 1997;40:466–9.Search in Google Scholar
15. Wei Y, Chen X, Jiang X, Ma Z, Xiao J. Determination of taxifolin in Polygonum orientale and study on its antioxidant activity. J Food Compos Anal 2009;22:154–7.10.1016/j.jfca.2008.08.006Search in Google Scholar
16. Nigam V, Patel A, Malvi R, Gupta B, Vikram P, Goyanar G. Antihyperglycaemic activity on flower of Polygonum orientale Linn. using steptozotocin induced diabetic mice model. Int J Res Dev Pharm Life Sci 2013;4:2626–31.Search in Google Scholar
17. Liao SG, Li YT, Zhang LJ, Wang Z, Chen TX, Huang Y, et al. UPLC-PDA-ESI-MS/MS analysis of compounds extracted by cardiac h9c2 cell from Polygonum orientale. Phytochem Anal 2013;24:25–35.10.1002/pca.2374Search in Google Scholar
18. Xiang MX, Xu L, Liu Y, Yan YJ, Hu JY, Su HW. In vitro evaluation of the effects of Polygonum orientale L. on proliferation and differentiation of osteoblastic MC3T3-E1 cell. J Med Plants Res 2011;5:231–6.Search in Google Scholar
19. Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Sarsf MN. Membrane stabilization activity – a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia 1989;70:251–7.10.1016/S0367-326X(99)00030-1Search in Google Scholar
20. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169–73.10.1111/j.2042-7158.1968.tb09718.xSearch in Google Scholar
21. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci 2010;2:146–55.Search in Google Scholar
22. Shoba FG, Thomas M. Study of anti-diarrhoeal activity of four medicinal plants in castor oil induced diarrhea. J Ethnopharmacol 2001;76:73–6.10.1016/S0378-8741(00)00379-2Search in Google Scholar
23. Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF. Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thromb J 2006;4:14.10.1186/1477-9560-4-14Search in Google Scholar PubMed PubMed Central
24. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982;45:31–4.10.1055/s-2007-971236Search in Google Scholar PubMed
25. Rahman MA, Sultana R, Emran TB, Islam MS, Rahman MA, Chakma JS, et al. Effect of organic extracts on six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC Complement Altern Med 2013;13:25.10.1186/1472-6882-13-25Search in Google Scholar PubMed PubMed Central
26. Rashid U, Khan MR, Jan S, Bokhari J, Shah NA. Assessment of phytochemicals, antimicrobial and cytotoxic activities of extract and fractions from Fagonia olivieri (Zygophyllaceae). BMC Complement Altern Med 2013;13:167.10.1186/1472-6882-13-167Search in Google Scholar PubMed PubMed Central
27. Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF. Effect of Fagonia arabica (Dhamasa) on in vitro thrombolysis. BMC Complement Altern Med 2007;7:36.10.1186/1472-6882-7-36Search in Google Scholar PubMed PubMed Central
28. Veermuthu D, Muniappan A, Savarimuthu I. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamilnadu, India. BMC Complement Altern Med 2006;6:35.10.1186/1472-6882-6-35Search in Google Scholar PubMed PubMed Central
29. Rahman MM, Hossain MA, Siddique SA, Biplab KP, Uddin MH. Antihyperglycemic, antioxidant, and cytotoxic activities of Alocasia macrorrhizos (L.) rhizome extract. Turk J Biol 2012;36: 574–9.10.3906/biy-1112-11Search in Google Scholar
30. Mollik MA, Hossan MS, Paul AK, Rahman MT, Jahan R, Rahmatullah M. A comparative analysis of medicinal plants used by folk medicinal healers in three districts of Bangladesh and inquiry as to mode of selection of medicinal plants. Ethnobotany Res Appl 2010;8:195–218.10.17348/era.8.0.195-218Search in Google Scholar
31. Anosike CA, Obidoa O, Ezeanyika LU. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). Daru J Pharm Sci 2012;20:76.10.1186/2008-2231-20-76Search in Google Scholar PubMed PubMed Central
32. Feirrali M, Signormi C, Ciccolili L, Comporti M. Iron release and membrane damage in erythrocytes exposed to oxidizing agents, phenylhydrazine, devicene and iso-uranil. Biochem J 1992;285:295–301.10.1042/bj2850295Search in Google Scholar PubMed PubMed Central
33. Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 2004;142:231–55.10.1038/sj.bjp.0705776Search in Google Scholar
34. Chaitanya R, Sandhya S, David B, Vinod KR, Murali S. HRBC membrane stabilizing property of root, stem and leaf of Glochidion velutinum. Int J Res Pharmaceut Biomed Sci 2011;2:256–9.Search in Google Scholar
35. Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziura P, Nkeh-Chungag BN, et al. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J Med Plants Res 2010;4:789–95.Search in Google Scholar
36. Mizushima Y. Inhibition of protein denaturation by antirheumatic and antiphlogistic agents. Arch. Int Pharmacodyn Ther 1964;149:1–7.Search in Google Scholar
37. Vane JR. Inhibition of prostaglandins synthesis as a mechanism of action for aspirin-like drugs. Nature 1971;231:232–5.10.1038/newbio231232a0Search in Google Scholar
38. Ali MK, Ashraf A, Biswas NN, Karmakar UK, Afroz S. Antinociceptive, anti-inflammatory and antidiarrheal activities of ethanolic calyx extract of Hibiscus sabdariffa Linn. (Malvaceae) in mice. J Chin Integr Med 2011;9:626–31.10.3736/jcim20110608Search in Google Scholar
39. Chitme HR, Chandra R, Kaushik S. Studies on anti-diarrheal activity of Calotropis gigantean in experimental animals. J Pharmacol Pharm Sci 2004;7:70–5.Search in Google Scholar
40. Ezenwali MO, Njoku OU, Okoli CO. Studies on the anti-diarrhoeal properties of seed extract of Monodora tenuifolia. Int J Appl Res Nat Prod 2009;2:20–6.Search in Google Scholar
41. Meite SN, Guessan JD, Bahi C, Yapi HF, Djaman AJ, Guina FG. Antidiarrheal activity of the ethyl acetate extract of Morinda morindoides in rats. Trop J Pharm Res 2009;8:201–7.10.4314/tjpr.v8i3.44533Search in Google Scholar
42. Shah S. Evaluation of diarrhea. The challenge continues! Part-1. Indian J Med Sci 2004;58:75–8.Search in Google Scholar
43. Pantzar M, Ljungh A, Wadstrom T. Plasminogen binding and activation at the surface of Helicobacter pylori CCUG 17874. Infect Immun 1998;66:4976–80.10.1128/IAI.66.10.4976-4980.1998Search in Google Scholar
44. Ali MS, Amin MR, Kamal CM, Hossain MA. In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves. Asian Pac J Trop Biomed 2013;3:464–9.10.1016/S2221-1691(13)60097-0Search in Google Scholar
45. Singh A, Singh SK. Molluscicidal evaluation of three common plants from India. Fitoterapia 2005;76:747–51.10.1016/j.fitote.2005.08.002Search in Google Scholar PubMed
46. Fowles RG, Mootoo BS, Ramsewak RS, Khan A. Toxicity-structure activity evaluation of limonoids from Swietenia species on Artemia salina. Pharm Biol 2012;50:264–7.10.3109/13880209.2011.581670Search in Google Scholar PubMed
47. Logarto PA, Silva YR, Guerra SI, Iglesias BL. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine 2001;8:395–400.10.1078/0944-7113-00044Search in Google Scholar PubMed
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