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Licensed Unlicensed Requires Authentication Published by De Gruyter August 3, 2016

Anti-inflammatory, anti-diarrheal, thrombolytic and cytotoxic activities of an ornamental medicinal plant: Persicaria orientalis

  • Prawej Ansari , Md. Josim Uddin EMAIL logo , Md. Masudur Rahman , Md. Abdullah-Al-Mamun , Md. Rabiul Islam , Md. Hazrat Ali and A.S.M. Ali Reza



Persicaria orientalis, an ornamental medicinal plant, has been used in traditional medicine for the treatment of various diseases. Although the plant is reported to have some important pharmacological effects, many medicinal values remain unidentified. Our objective was to evaluate the anti-inflammatory, anti-diarrheal, thrombolytic, and cytotoxic properties of the methanol extract of P. orientalis leaves (Po-MeOH).


Anti-inflammatory activity was measured by the inhibition of hypotonicity-induced human red blood cell hemolysis and albumin denaturation technique in vitro of Po-MeOH. Diarrheal episodes were examined in mice with castor oil-induced diarrhea. The clot lysis and brine shrimp lethality bioassay in vitro were used to evaluate the thrombolytic and cytotoxic activities of the plant extract, respectively.


Using in vitro anti-inflammatory models, the results demonstrated that Po-MeOH at the five different dose ranges from 31.25 to 500 μg/mL significantly (p<0.05) protected (0.98%–50.71%) the erythrocyte membrane against lysis induced by hypotonic medium solution and protein denaturation (38.27%–79.22%) of bovine albumin, respectively. The extract exhibited a significant reduction of severity (75.17%) of castor oil-induced diarrhea in mice at the highest dose of 400 mg/kg compared to loperamide (82.06%) at 5 mg/kg. Po-MeOH also showed 33.14% clot lytic activity in the thrombolytic test and cytotoxicity with LC50 value 58.91 μg/mL in the brine shrimp bioassay.


These findings suggest that Po-MeOH has significant anti-inflammatory and anti-diarrheal effects along with moderate thrombolytic and lower cytotoxic properties that may warrant the further exploration.


We are grateful to the Department of Pharmacy, International Islamic University Chittagong, Bangladesh, for providing facilities for this research work.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. MJU and PA conceived and designed the experiments. PA, MJU, and MA carried out the anti-inflammatory and anti-diarrheal tests. MRI, MHA, and AR conducted the thrombolytic and cytotoxicity experiments. Under the supervision of MJU wrote the manuscript. MJU and MMR performed statistical analysis. All authors read and approved the final manuscript.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


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Received: 2016-2-22
Accepted: 2016-6-24
Published Online: 2016-8-3
Published in Print: 2017-1-1

©2017 Walter de Gruyter GmbH, Berlin/Boston

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