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Licensed Unlicensed Requires Authentication Published by De Gruyter March 29, 2017

Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats

  • Anthoneth Ndidi Ezejiofor , Zelinjo Nkeiruka Igweze , Nnaemeka Arinze Udowelle and Orish Ebere Orisakwe EMAIL logo



In continuation of our study of the biological activity of the aqueous extract of Costus afer stem used in tradomedicare in Nigeria, this work has investigated the biochemical, nephroprotective, hepatoprotective and protective effects of aqueous extract of C. afer stem on alloxan-induced hyperglycaemic albino Wistar rats.


Thirty male albino Wistar rats (150−200 g) were weight-matched into six groups of five rats each. Groups 1 and 2 were the normal (non-induced) and toxic (alloxan-induced) controls, respectively. Groups 3−6 were induced and treated with 1, 2 and 3 g/kg of the aqueous extract of C. afer stem and glibenclamide (5 mg/kg), respectively. Food and fluid intake, body weight, absolute and relative weight of organs (liver, pancreas, and kidneys) and biochemical parameters were measured.


There was a significant reduction in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin triglyceride, creatinine, urea and electrolyte when compared with toxic control group. The liver marker enzymes were restored in all the treated groups. The aqueous stem extract possessed hypoglycaemic effect and reversed the histopathological damage in alloxan-induced diabetic rats.


This study suggests that aqueous stem extract of C. afer possesses both hypoglycaemic and organ protective properties.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Jimoh A, Tanko Y, Mohammed A. Anti-diabetic effect of methanolic leaf of cissus cornifolia on alloxan induced hyperglycaemic wistar rats. Ann Biol Res 2013;4:46–54.Search in Google Scholar

2. Babitha S, Banji D, Banji OJ. Antioxidant and hepatoprotective effects of flower extract of Millingtonia hortensis Linn. on carbon tetrachloride induced hepatotoxicity. J Pharm Bioallied Sci 2012;4:307–12.10.4103/0975-7406.103258Search in Google Scholar

3. Kidd PM. Glutathione (Monograph). In: Czap K, Miller AL, Head KA, et al., editors. Alternative medicine review monographs volume one. Dover, ID: Thorne Research, Inc., 2002: 184–92.Search in Google Scholar

4. Iwu MM. Handbook of African medicinal plants [M]. Boca Raton, FL, USA: CRC Press, 1993:464.Search in Google Scholar

5. Oliver-Bever BE. Medicinal plants in tropical West Africa. Cambridge, UK: Cambridge University Press, 1986.10.1017/CBO9780511753114Search in Google Scholar

6. Ezejiofor AN, Orish CN, Orisakwe OE. Effect of aqueous leaves extract of Costus afer Ker Gawl (Zingiberaceae) on the liver and kidney of male albino Wistar rat. Ancient Sci Life 2013;33:4–9.10.4103/0257-7941.134554Search in Google Scholar

7. Nandhakumar J, Sethumathi PP, Malini A, Sengottuvelu S, Duralsamy R, Karthikeyan D, et al. Antidiabetic activity of methanol leaf extract of costus pictus D. Don in alloxan induced diabetic rats. J Health Sci 2007;53:655–63.10.1248/jhs.53.655Search in Google Scholar

8. Jayasri MA, Gunasekaran S, Radha A, Mathew TL. Antidiabetic effect of Costus pictus leaves in normal and streptozotocin-induced diabetic rats. J Diabetes Metab 2008;16:117–22.Search in Google Scholar

9. Udem SC, Ezeasor CK. The acute and subchronic toxicity studies of aqueous leaf and stem bark extract of Costus afer ker (zingiberaceae) in mice. Comp Clin Pathol 2010;19: 75–80.10.1007/s00580-009-0906-8Search in Google Scholar

10. Reyes BA, Bautista ND, Tanquilut NC, Anunciado RV, Leung AB, Sanchez GC, et al. Anti-diabetic potentials of Momordica charantia and Andrographis paniculata and their effects on estrous cyclicity of alloxan-induced diabetic rats. J Ethnopharmacol 2006;105:196–200.10.1016/j.jep.2005.10.018Search in Google Scholar

11. Mahmoudzadeh-Sagheb H, Heidari Z, Shahraki M, Moudi B. A stereological study of effects of aqueous extract of Tamarindus indica seeds on pancreatic islets in streptozotocin-induced diabetic rats. Pak J Pharm Sci 2010;23:427–34.Search in Google Scholar

12. Abdel-Barry JA, Abdel-Hassan IA, Al-Hakeim MHH. Hypoglycaemic and antihyperglycaemic effects of Trigonella foenum-graecum leaf in normal and alloxan induced diabetic rats. J Ethnopharmacol 1997;58:149–55.10.1016/S0378-8741(97)00101-3Search in Google Scholar

13. Nannipieri M, Gonzales C, Baldi S, Posadas R, Williams K, Haffner SM, et al. Liver enzymes, metabolic syndrome, and incident diabetes: the Mexico City diabetes study. Diabetes Care 2005;28:1757–62.10.2337/diacare.28.7.1757Search in Google Scholar PubMed

14. Villegas R, Xiang YB, Elasy T, Cai Q, Xu W, Li H, et al. Liver enzymes, type 2 diabetes, and metabolic syndrome in middle-aged, urban Chinese men. Metab Syndr Relat Disord 2011;9:305–11.10.1089/met.2011.0016Search in Google Scholar PubMed PubMed Central

15. Najmi AK, Pillai KK, Pal SN, Aqil M. Free radical scavenging and hepatoprotective activity of jigrine against galactosamine induced hepatotoxicity in rats. J Ethnopharmacol 2005;97: 521–5.10.1016/j.jep.2004.12.016Search in Google Scholar PubMed

16. Rajesh V, Perumal P, Sundarrajan T. Antidiabetic activity of methanol extract of Smilax zeylanica Linn in streptozotocin induced diabetic rats. Intern J Pharmacol 2010;6. Available at: in Google Scholar

17. Felig P, Mark E, Ohman JL, Lahil CF. Plasma amino acids level in diabetic ketoacidosis. Diabetes 1970;19:727–8.10.2337/diab.19.10.727Search in Google Scholar

18. Larson RA. The antioxidants of higher plants. Phytochemistry 1988;27:969–78.10.1016/0031-9422(88)80254-1Search in Google Scholar

Received: 2016-3-19
Accepted: 2017-2-7
Published Online: 2017-3-29
Published in Print: 2017-7-26

©2017 Walter de Gruyter GmbH, Berlin/Boston

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