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Licensed Unlicensed Requires Authentication Published by De Gruyter February 8, 2018

Dose-dependent effects of vitamin 1,25(OH)2D3 on oxidative stress and apoptosis

  • Cagri Cakici , Turkan Yigitbasi EMAIL logo , Sule Ayla , Hadi Karimkhani , Feyza Bayramoglu , Pakize Yigit , Ertugrul Kilic and Nesrin Emekli



The purpose of this study is to examine the dose-dependent effects of vitamin 1,25(OH)2D3 on apoptosis and oxidative stress.


In this study, 50 male Balb/c mice were used as control and experiment groups. The mice were divided into 5 groups each consisting of 10 mice. Calcitriol was intraperitoneally administered as low dose, medium dose, medium-high dose and high dose vitamin D groups (at 0.5, 1, 5 and 10 μg/kg, respectively), for three times a week during 14 days. At the end of the study, annexin V was measured by enzyme-linked immunosorbent assay method, and total antioxidant capacity and total oxidant status values were measured by colorimetric method in serum. Hematoxylin eosin staining was performed in liver tissues and periodic acid schiff staining was performed in kidney tissues.


While comparing the results of medium-high dose (5 μg/kg) and high dose (10 μg/kg) vitamin D administration to that of the control group, it was observed that serum antioxidant status and annexin V levels decreased and glomerular mesenchial matrix ratio increased in kidney (p<0.05). In addition to these findings, in the group receiving high dose vitamin D (10 μg/kg), it was observed that the damage to the liver increased together with the the oxidative stress index values (p<0.05).


As a result, this study was the first in the literature to report that use of high-dose vitamin D (10 μg/kg) results in oxidant effect, rather than being an antioxidant, and causes severe histopathological toxicity in the liver and kidney.


We would like to special thank MSc. Ozge Biceroglu and MSc. Tugce Onel from Histology Department at the Medipol University who assisted with histopathological studies.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was funded by a grant from the Istanbul Medipol University (Grant no. BAP-2016/17).

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


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Received: 2017-8-4
Accepted: 2017-12-7
Published Online: 2018-2-8
Published in Print: 2018-6-27

©2018 Walter de Gruyter GmbH, Berlin/Boston

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