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Licensed Unlicensed Requires Authentication Published by De Gruyter September 28, 2018

Correlation between the blood level of irisin and the severity of acute myocardial infarction in exercise-trained rats

Shaimaa M. Bashar , Shereen M. Samir El-sherbeiny EMAIL logo and Mohamed Z. Boraie



Acute myocardial infarction is a major cause of death all over the world. Irisin is a novel myokine released after exercise. This work aimed to study the correlation between the serum irisin level and the severity of the acute myocardial infarction in the exercise-trained rats.


Forty-eight male rats were classified into four groups (12 for each): group I, control sedentary (C); group II, exercise-trained (EX) (swimming for 8 weeks); group III, isoprenaline-induced infarct (MI); and group IV, exercise-trained infarct (EX-MI) (swimming for 8 weeks followed by isoprenaline-induced infarction). ECG was recorded at start and end of the study, before and after induction of infarction. The serum level of irisin, lipid peroxidation [malondialdehyde (MDA)], total antioxidant status (TAS), creatine phosphokinase-MB (CK-MB), and troponin I was determined. The hearts were excised for histopathology and immunohistochemistry for caspase-3.


The infarct rats showed significant prolongation in QTc interval and elevation in the ST segment as well as significant elevation of serum CK-MB, troponin I, and MDA, whereas TAS and serum irisin level were significantly decreased. With exercise, we observed a high positive correlation between the serum irisin and QRS duration (+0.643), amplitude (+0.860), and TAS (+0.887). In addition, there was a high negative correlation between the serum irisin and ST elevation (−0.865), QTc (−0.886), CK-MB (−0.891), troponin (−0.882), and MDA (−0.868). This was confirmed by the negative correlation between serum irisin and both collagen deposition and caspase-3 expression (–0.823 and –0.822, respectively).


We recommend regular exercise or taking recombinant irisin as a supplement to protect at-risk individuals against acute myocardial infarction.


The Physiology Department and Medical Experimental Research Center of Mansoura University are acknowledged for their significant contribution to the experimental part of the study.

  1. Author contribution: Shereen M. Samir, Shaimaa M. Bashar, and Mohamed Z. Boraie designed and performed research, wrote the paper, and analyzed the data.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Ahmed E, Al Suwaidi J, El-Menyar A, Al Binali H, Singh R, Gehani A. Mortality trends in patients hospitalized with the ınitial acute myocardial ınfarction in a Middle Eastern country over 20 years. Cardio Res Pract 2014;28:1–8.10.1155/2014/464323Search in Google Scholar PubMed PubMed Central

2. Aydin S, Aydin S. Irisin concentrations as a myocardial biomarker. Biomark Cardio Dis 2016;489–504. DOI 10.1007/978-94-007-7678-4_3.10.1007/978-94-007-7678-4_3Search in Google Scholar

3. White M, Edwards A, Cordwell S, Van Eyk J. Mitochondria: a mirror into cellular dysfunction in heart disease. Proteom Clin Appl 2008;2:845–61.10.1002/prca.200780135Search in Google Scholar PubMed

4. Chiong M, Wang Z, Pedrozo Z, Cao D, Troncoso R. Cardiomyocyte death: mechanisms and translational implications. Cell Death Dis 2011;2:e244.10.1038/cddis.2011.130Search in Google Scholar PubMed PubMed Central

5. Boström P, Wu J, Jedrychowski M, Korde A, Ye L. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012;481:463–8.10.1038/nature10777Search in Google Scholar PubMed PubMed Central

6. Roca-Rivada A, Castelao C, Senin L, Landrove M, Baltar J, Belén Crujeiras A. Irisin is not only a myokine but also an adipokine. PLoS One 2013;8:e60563.10.1371/journal.pone.0060563Search in Google Scholar PubMed PubMed Central

7. Boström P, Fernández-Real J. Irisin, the metabolic syndrome and follistatin in humans. Nat Rev Endocrinol 2014;10:11–2.10.1038/nrendo.2013.230Search in Google Scholar PubMed

8. Lu Y, Li H, Shen S, Shen Z, Xu M. Swimming exercise increases serum irisin level and reduces body fat mass in high fat-diet fed Wistar rats. Lip Health Dis 2016;15:93.10.1186/s12944-016-0263-ySearch in Google Scholar PubMed PubMed Central

9. Huh J, Panagiotou G, Mougios V, Brinkoetter M, Vamvini M. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism 2012;61:1725–38.10.1016/j.metabol.2012.09.002Search in Google Scholar PubMed PubMed Central

10. Liu J, Wong M, Toy W, Tan C, Liu S. Lower circulating irisin is associated with type 2 diabetes mellitus. J Diabetes Complications 2013;27:365–9.10.1016/j.jdiacomp.2013.03.002Search in Google Scholar PubMed

11. Song H, Wu F, Zhang Y, Wang F, Jiang M. Irisin promotes human umbilical vein endothelial cell proliferation through the ERK signaling pathway & partly suppresses high glucose-induced apoptosis. PLoS One 2014;9:e110273.10.1371/journal.pone.0110273Search in Google Scholar PubMed

12. Zhu G, Wang J, Song M, Zhou F, Fu D. Irisin increased number and improved the function of endothelial progenitor cells in diabetes mellitus mice. J Cardiovasc Pharmacol 2016;68:67–73.10.1097/FJC.0000000000000386Search in Google Scholar PubMed

13. Moon H, Dincer F, Mantzoros C. Pharmacological concentrations of irisin increase cell proliferation without influencing markers of neurite outgrowth and synaptogenesis in mouse H19-7 hippocampal cell lines. Metabolism 2013;62:1131–6.10.1016/j.metabol.2013.04.007Search in Google Scholar PubMed

14. Zhang Y, Mu Q, Zhou Z, Song H, Wu F. Protective effect of irisin on atherosclerosis via suppressing oxidized low-density lipoprotein induced vascular inflammation & endothelial dysfunction. PLoS One 2016;11:e0158038.10.1371/journal.pone.0158038Search in Google Scholar PubMed

15. Kuloglu T, Aydin S, Eren M, Yilmaz M, Sahin I, Kalayci M. Irisin: a potentially candidate marker for myocardial infarction. Peptides 2014;55:85–91.10.1016/j.peptides.2014.02.008Search in Google Scholar PubMed

16. Aronis K, Moreno M, Polyzos S, Moreno-Navarrete J, Ricart W. Circulating irisin levels and coronary heart disease: association with future acute coronary syndrome and major adverse cardiovascular events. Int J Obes (Lond) 2015;39:156–61.10.1038/ijo.2014.101Search in Google Scholar PubMed

17. Li H, Shen Z, Lu Y, Lin F, Wu Y, Jiang Z. Muscle NT-3 levels increased by exercise training contribute to the improvement in caudal nerve conduction velocity in diabetic rats. Mol Med Rep 2012;6:69–74.Search in Google Scholar PubMed

18. Prasad K. Hypochloesterolemic and antiatherosclerotic effect of flax lignan complex isolated from flaxseed. Atherosclerosis 2015;179:269–75.10.1016/j.atherosclerosis.2004.11.012Search in Google Scholar

19. Mutiso S, Rono D, Bukachi F. Relationship between anthropometric measures and early electrocardiographic changes in obese rats. BMC Res Notes 2014;7:931.10.1186/1756-0500-7-931Search in Google Scholar PubMed

20. Phan D, Silka M, Lan Y, Chang R. Comparison of formulas for calculation of the corrected QT interval in infants and young children. J Pediatr 2015;166:960–4.10.1016/j.jpeds.2014.12.037Search in Google Scholar PubMed

21. Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chem Acta 1978;90:37–43.10.1016/0009-8981(78)90081-5Search in Google Scholar

22. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001;54:356–61.10.1136/jcp.54.5.356Search in Google Scholar PubMed PubMed Central

23. Lobo Filho H, Ferreira N, de Sousa R, de Carvalho E. Experimental model of myocardial infarction induced by isoproterenol in rats. Braz J Cardiol Sur 2011;26:469–76.10.5935/1678-9741.20110024Search in Google Scholar PubMed

24. Abbas A. Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats. J Physiol Biochem 2016;72:469–84.10.1007/s13105-016-0494-4Search in Google Scholar PubMed

25. Lin J, Hsu S, Wu S, Teng M, Chou H, Cheng S. QT interval independently predicts mortality and heart failure in patient with ST elevation myocardial infarction. Int J Med Sci 2015;12:968–73.10.7150/ijms.13121Search in Google Scholar PubMed PubMed Central

26. Coppola G, Carità P, Corrado E, Borrelli A, Rotolo A, Guglielmo M. ST segment elevations: always a marker of acute myocardial infarction? Indian Heart J 2013;65:412–23.10.1016/j.ihj.2013.06.013Search in Google Scholar PubMed PubMed Central

27. Punithavathi V, Prince S. The cardioprotective effects of a combination of quercetin and α-tocopherol on isoproterenol-induced myocardial infarcted rats. J Biochem Mol Toxicol 2011;25:28–40.10.1002/jbt.20357Search in Google Scholar PubMed

28. Sharmila S, Rajadurai M. Preventive effect of Bio-Aq on cardiac markers, lipids, and membrane bound enzymes in isoproterenol-induced myocardial infarction in rats. Asian J Pharm Clin Res 2012;5:107–13.Search in Google Scholar

29. Saravanan G, Ponmurugan P, Sathiyavathi M, Vadivukkarasi S, Sengottuvelu S. Cardioprotective activity of Amaranthus viridis Linn: effect on serum marker enzymes, cardiac troponin and antioxidant system in experimental myocardial infarcted rats. Int J Cardiol 2013;165:494–8.10.1016/j.ijcard.2011.09.005Search in Google Scholar PubMed

30. Khalil M, Tanvir E, Afroz R, Sulaiman S, Gan S. Cardioprotective effects of tualang honey: amelioration of cholesterol and cardiac enzymes levels. BioMed Res Int 2015;1–8. DOI 10.1155/2015/286051.10.1155/2015/286051Search in Google Scholar PubMed PubMed Central

31. Wang H, Zhao Y, Zhang S, Dubielecka P, Du J, Yano N. Irisin plays a pivotal role to protect the heart against ischemia and reperfusion injury. J Cell Physiol 2017;232:3775–85.10.1002/jcp.25857Search in Google Scholar PubMed PubMed Central

32. DeNicola M, Du J, Wang Z, Yano N, Zhang L, Wang Y, et al. Stimulation of glucagon-like peptide-1 receptor through exendin-4 preserves myocardial performance and prevents cardiac remodeling in infarcted myocardium. Am J Physiol Endocrinol Metab 2014;307:630–43.10.1152/ajpendo.00109.2014Search in Google Scholar PubMed PubMed Central

33. De Nigris F, Demontis M, Rodriguez-Porcel M, Anania V, Lerman L, Napoli C. Renal blood flow in hypercholesterolemic pigs is increased by chronic antioxidant treatment. J Vet Pharmacol Ther 2003;26:113–6.10.1046/j.1365-2885.2003.00461.xSearch in Google Scholar PubMed

34. Priscilla D, Prince P. Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact 2009;179:118–24.10.1016/j.cbi.2008.12.012Search in Google Scholar PubMed

35. Alcerczyk A, Bartosz G. Thiols are main determinants of total antioxidant capacity of cellular homogenates. Free Radic Res 2003;37:537–41.10.1080/1071576031000083189Search in Google Scholar PubMed

36. Tao L, Bei Y, Lin S, Zhang H. Exercise training protects against acute myocardial infarction via improving myocardial energy metabolism and mitochondrial biogenesis. Cell Physiol Biochem 2015;37:162–75.10.1159/000430342Search in Google Scholar PubMed

37. Sugiyama Y, Asai K,Yamada K, Kureya Y, Ijiri N. Decreased levels of irisin, a skeletal muscle cell-derived myokine, are related to emphysema associated with chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis 2017;12:765–72.10.2147/COPD.S126233Search in Google Scholar PubMed PubMed Central

38. Saing L, Wei Y, Tseng C. Ergothioneine represses inflammation and dysfunction in human endothelial cells exposed to oxidized low-density lipoprotein. Clin Exp Pharmacol Physiol 2015;43:720.Search in Google Scholar

39. Karthick M, Prince P. Preventive effect of rutin, a bioflavonoid, on lipid peroxides and antioxidants in isoproterenol-induced myocardial infarction in rats. J Pharm Pharmacol 2006;58: 701–7.10.1211/jpp.58.5.0016Search in Google Scholar PubMed

40. Sun S, Wu X, Song H, Li G. Baicalin ameliorates isoproterenol-induced acute myocardial infarction through INOS, inflammation, oxidative stress and P38MAPK pathway in rat. Int J Clin Exp Med 2015;8:22063.Search in Google Scholar PubMed

41. Haibo S, Fei W, Yuan Z, Yuzhu Z, Fang W. Irisin promotes human umbilical vein endothelial cell proliferation through the ERK signaling pathway and partly suppresses high glucose-induced apoptosis. PLoS One 2014;9:e110273.10.1371/journal.pone.0110273Search in Google Scholar PubMed PubMed Central

Received: 2018-05-08
Accepted: 2018-08-04
Published Online: 2018-09-28
Published in Print: 2018-12-19

©2019 Walter de Gruyter GmbH, Berlin/Boston

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