Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter January 23, 2020

Effect of curcumin analogue synthetic product from cullilawan oil for the liver damage treatment in male mice (Mus musculus L.)

  • Imanuel Berly Delvis Kapelle EMAIL logo , Wasmen Manalu and Fensia Analda Souhoka


The active component in cullilawan oil can be synthesized into curcumin analogue product, which has pharmacological activity. The synthesis process by using conventional and microwave methods can produce different isomer products. Different synthesis products and models of animal are used to provide different hepatoprotective effects. The aim of this study was to use the curcumin analogue synthetic products (AKS-k and AKS-m) from cullilawan oil in male mice (Mus musculus L.) liver damage treatment induced by carbon tetrachloride (CCl4). The in vivo method was employed using biochemical of blood and histopathological images of liver cells as indicators. The results showed that the curcumin analogue synthetic product using microwave methods had better pharmacological effects than the conventional method product in terms of the results of blood biochemical analysis and microscopic images of liver cells.

  1. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  2. Research funding: This research fund is supported by Directorate of Research and Community Service, The Ministry of Research, Technology, and Higher Education for giving Research Post-Doctoral.

  3. Competing interests: The authors state no conflict of interest.

  4. Ethical approval: Research involving animals complied with all relevant national regulations and institutional policies for the care and use of animals (614/ETIK2019).


[1] Kapelle IB, Irawadi TT, Rusli MS, Mangunwidjaja D, Mas’ud ZA. Rekayasa Proses Sintesis Piperonal Kulit Lawang (Cinnamomum cullilawan Blume) Sebagai Prekursor Obat Kanker. Jurnal Penelitian Hasil Hutan 2016;34:217–29.10.20886/jphh.2016.34.3.217-229Search in Google Scholar

[2] Kapelle IB, Irawadi TT, Rusli MS, Mangunwidjaja D, Mas’ud ZA. Synthesis of new curcumin analogs from kulit lawang oils using the conventional method and microwave. Sci J Chem 2015;3:50–6.10.11648/j.sjc.20150303.13Search in Google Scholar

[3] Kapelle IB, Irawadi TT, Rusli MS, Mangunwidjaja D, Mas’ud ZA. Synthesis of asymmetric curcumin analogs from cullilawan oil using conventional and microwave method. Procedia Chem 2015;16:480–8.10.1016/j.proche.2015.12.082Search in Google Scholar

[4] Kapelle IB, Irawadi TT, Rusli MS, Mangunwidjaja D, Mas’ud ZA. The influence of synthesis methods against anti-cancer activity of curcumin analogous. Cancer Res J 2015;3:68–75.10.11648/j.crj.20150304.12Search in Google Scholar

[5] Yang CH, Yue J, Sims M, Pfeffer LM. The curcumin analog EF24 targets NF-kB and miRNA-21, and has potent anticancer activity in vitro and in vivo. PLoS One 2013;8:e71130.10.1371/journal.pone.0071130Search in Google Scholar PubMed PubMed Central

[6] Johnson JJ, Mukhtar H. Curcumin for chemoprevention of colon cancer. Cancer Let 2007;255:170–81.10.1016/j.canlet.2007.03.005Search in Google Scholar PubMed

[7] Khan RA, Khan MR, Ahmed M, Sahreen S, Shah NA, Shah MS, et al. Hepatoprotection with a chloroform extract of Launaea procumbens against CCl4-induced injuries in rats. BMC Complement Altern Med 2012;12:114.10.1186/1472-6882-12-114Search in Google Scholar PubMed PubMed Central

[8] Kapelle IB, Manalu W. The preventive effect of curcumin analogs (AKS-k) from cullilawan oil as hepatoprotector in rats (Rattus norvegicus L.) induced by CCl4. IJPSAT 2018;11:81–5.Search in Google Scholar

[9] Burt AD, Portmann BC, Ferrel LB. Pathology of the liver, 5th ed. New York: Churchill Livingstone, 2007.Search in Google Scholar

[11] Ruhu P, McDonald R. Use of antioxidant nutrient in the prevention and treatment of type 2 diabetes. J Am Coll Nutr 2001;20:363–9.10.1080/07315724.2001.10719169Search in Google Scholar PubMed

[13] Calce E, Bugatti V, Vittoria V, De Luca S. Solvent-free synthesis of modified pectin compounds promoted by microwave irradiation. Molecules 2012;17:12234–42.10.3390/molecules171012234Search in Google Scholar PubMed PubMed Central

[14] Al-Mousawi SM, El-Apasery MA, Elnagdi MH. Microwave assisted dyeing of polyester fabrics with disperse dyes. Molecules 2013;18:11033–43.10.3390/molecules180911033Search in Google Scholar PubMed PubMed Central

[15] Motoo T. Relative stability of cis- and trans-hydrindanones. Molecules 2015;20:1509–18.10.3390/molecules20011509Search in Google Scholar PubMed PubMed Central

[16] Hickey T, Claffey J, Fitzpatrick E, Hogan M, Pampillón C, Tacke M. Dimethylamino-functionalised and N-heteroaryl-substituted titanocene anticancer drugs: synthesis and cytotoxicity studies. Invest New Drugs 2007;25:425–33.10.1007/s10637-007-9061-8Search in Google Scholar PubMed

[17] Lullman H, Lutz H, Klaus M, Detlef B. Color atlas of pharmacology, 3rd ed, revised and expanded. Stuttgart and New York: Thieme, 2005.Search in Google Scholar

Received: 2019-09-02
Accepted: 2019-11-04
Published Online: 2020-01-23

© 2019 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 10.6.2023 from
Scroll to top button