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Licensed Unlicensed Requires Authentication Published by De Gruyter December 20, 2019

Coenzyme Q10 nanostructured lipid carriers as an inducer of the skin fibroblast cell and its irritability test in a mice model

Fenita Shoviantari ORCID logo, Tristiana Erawati and Widji Soeratri

Abstract

Background

Coenzyme Q10 is a fat-soluble antioxidant that can help to prevent collagen and elastin damage and avoid wrinkles. Coenzyme Q10 has several disadvantages to be formulated in topical dosage forms, such as low water solubility and large molecular weight. These make coenzyme Q10 retained in the stratum corneum and cause low skin penetration, so proper formulation is required to get products that can penetrate the skin layer. A nanostructured lipid carrier (NLC) consists of a matrix of solid lipids and liquid lipids in a certain amount with nanoparticle size; it may help increase the penetration of active ingredients.

Methods

For the antiaging activity test, mice were grouped into four treatment groups and killed on the 14th day; then the back of the skin was stained with Masson trichrome staining. For the irritation test, the mice were grouped into three groups and killed after 24 h; then the back of the mice was stained with hematoxylin-eosin staining.

Results

The number of fibroblasts in mice with NLC coenzyme Q10 is highest from all test groups. The irritation test results after 24 h of application preparation showed that NLC coenzyme Q10 did not irritate the skin of the back of male mice.

Conclusions

One percent coenzyme Q10 loaded in NLC induced the number of fibroblast cells in the mice model and showed no irritability effect in histopathology preparations.

Acknowledgments

Thanks to Yayasan Bhakti Wiyata who allowed finishing and publishing this research. This paper has been presented at the 8th APPEN Conference and 2nd HPC Conference on 8 – 9 October 2019, Universitas Airlangga, Surabaya, Indonesia.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Ethical approval: All procedure of this research has been approved by The Animal Care and Use Committee of Universitas Airlangga, Indonesia, with Ethical Clearance number 645-KE.

References

[1] Ganceviciene R, Liakou AI, Theodoridis A, Makrantonaki E, Zouboulis CC. Skin anti-aging strategies. Dermato-Endocrinol 2012;4:308–19.10.4161/derm.22804Search in Google Scholar

[2] Burke KE. Implementation of cosmetic dermatology into therapeutic: antiaging regimens. In: Draelos DZ, editor(s). Cosmetic dermatology products and procedures. Durham, NC: Wiley Blackwell Publication, 2010:480–7.10.1002/9781444317657.ch59Search in Google Scholar

[3] Ramos-e-Silva M, Celem LR, Ramos-e-Silva S, Fucci-da-Costa AP. Anti-aging cosmetics: facts and controversies. Clin Dermatol 2013;31:750–8.10.1016/j.clindermatol.2013.05.013Search in Google Scholar

[4] Baumann LS. The Baumann skin typing system. In: Farage M, Miller K, Maibach H. editors. Textbook of aging skin. 2nd ed. New York: Springer, Berlin, Heidelberg, 2009:34–41.Search in Google Scholar

[5] Inui M, Ooe M, Fujii K, Matsunaka H, Yoshida M, Ichihashi M. Mechanisms of inhibitory effects of CoQ10 on UVB-induced wrinkle formation in vitro and in vivo. BioFactors 2008;32:237–43.10.1002/biof.5520320128Search in Google Scholar

[6] Lucangioli S, Tripodi V. The importance of the formulation in the effectiveness of coenzyme Q10 supplementation in mitochondrial disease therapy. Der Pharmacia Sinica 2012;3:406–7.Search in Google Scholar

[7] Korkmaz E, Gokceh E, Ozer O. Development and evaluation of coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced dermal delivery. Acta Pharm 2013;63:517–29.10.2478/acph-2013-0039Search in Google Scholar

[8] Müller RH, Radtke M, Wissing SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Deliv RevAdv . 2002;54:S131–55.10.1016/S0169-409X(02)00118-7Search in Google Scholar

[9] Puglia C, Blasi P, Rizza L, Schoubben A, Bonina F, Rossi C, et al. Lipid nanoparticles for prolonged topical delivery: an in vitro and in vivo investigation. Int J Pharm 2008;357:295–304.10.1016/j.ijpharm.2008.01.045Search in Google Scholar PubMed

[10] Phatak AA, Chaudhari PD. Development and evaluation of nanostructured lipid carrier (NLC) based topical delivery of an anti-inflammatory drug. J Pharm Res 2013;7:677–85.10.1016/j.jopr.2013.08.020Search in Google Scholar

[11] Kogan A, Garti N. Nanoemulsions as transdermal drug delivery vehicles. Adv Colloid Interface Sci 2006;123–6:369–85.10.1016/j.cis.2006.05.014Search in Google Scholar PubMed

[12] Derle DV. Essentials of physical pharmacy. Hyderabad [India]: PharmaMed Press. 2008;73–9.Search in Google Scholar

[13] Rowe RC, Sheskey PJ, Quinn ME. Handbook of pharmaceutical excipients, 6th ed. London: The Pharmaceutical Press, 2009:5–6; 17–9; 75–6; 389–90; 466–8; 470–2; 473–4; 549–53; 675–8.Search in Google Scholar

[14] Anwar E. Eksipien dalam Sediaan Farmasi Karakterisasi dan Aplikasi. Penerbit Dian Rakyat, Jakarta, 2012.Search in Google Scholar

[15] Kumar B, Jain SK, Prajapati SK, Mahor A, Kumar A. Development and characterization of transdermal microemulsion gel for an antiviral drug. Int J Pharm Sci Res 2010;1:57–73.Search in Google Scholar

[16] Santos P, Watkinson AC, Hadgraft J, Lane ME. Application of microemulsions in dermal and transdermal drug delivery. Skin Pharmacol Physiol 2008;21:246–59.10.1159/000140228Search in Google Scholar PubMed

[17] Shai A, Maibach H, Baran R. Skin structure. Handbook of cosmetic skin care. London: Informa Healtcare, 2009:4–13.10.3109/9781616310004.002Search in Google Scholar

Received: 2019-10-29
Accepted: 2019-11-15
Published Online: 2019-12-20

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