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Licensed Unlicensed Requires Authentication Published by De Gruyter December 20, 2019

Coenzyme Q10 nanostructured lipid carriers as an inducer of the skin fibroblast cell and its irritability test in a mice model

  • Fenita Shoviantari ORCID logo , Tristiana Erawati EMAIL logo and Widji Soeratri



Coenzyme Q10 is a fat-soluble antioxidant that can help to prevent collagen and elastin damage and avoid wrinkles. Coenzyme Q10 has several disadvantages to be formulated in topical dosage forms, such as low water solubility and large molecular weight. These make coenzyme Q10 retained in the stratum corneum and cause low skin penetration, so proper formulation is required to get products that can penetrate the skin layer. A nanostructured lipid carrier (NLC) consists of a matrix of solid lipids and liquid lipids in a certain amount with nanoparticle size; it may help increase the penetration of active ingredients.


For the antiaging activity test, mice were grouped into four treatment groups and killed on the 14th day; then the back of the skin was stained with Masson trichrome staining. For the irritation test, the mice were grouped into three groups and killed after 24 h; then the back of the mice was stained with hematoxylin-eosin staining.


The number of fibroblasts in mice with NLC coenzyme Q10 is highest from all test groups. The irritation test results after 24 h of application preparation showed that NLC coenzyme Q10 did not irritate the skin of the back of male mice.


One percent coenzyme Q10 loaded in NLC induced the number of fibroblast cells in the mice model and showed no irritability effect in histopathology preparations.


Thanks to Yayasan Bhakti Wiyata who allowed finishing and publishing this research. This paper has been presented at the 8th APPEN Conference and 2nd HPC Conference on 8 – 9 October 2019, Universitas Airlangga, Surabaya, Indonesia.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Ethical approval: All procedure of this research has been approved by The Animal Care and Use Committee of Universitas Airlangga, Indonesia, with Ethical Clearance number 645-KE.


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Received: 2019-10-29
Accepted: 2019-11-15
Published Online: 2019-12-20

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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