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Licensed Unlicensed Requires Authentication Published by De Gruyter June 25, 2021

The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics

  • Victoria Yulita Fitriani , Budi Suprapti EMAIL logo and Muhammad Amin

Abstract

Objectives

This study aims to determine the characteristics of Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk, respectively as probiotic candidate based on exposure to pH, bile salts, pathogenic bacteria, and antibiotics.

Methods

In vitro studies were conducted to examine the resistance of Lactobacillus acidophilus and Lactobacillus reuteri in pH 2, 2.5, 3.2, and 7.2, resistance to bile salts, resistance to pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Enterococcus faecalis) and antituberculosis antibiotics.

Results

Viability of Lactobacillus acidophilus and Lactobacillus reuteri isolates remained unchanged (6.3 × 107 CFU/mL and 5.03 × 107 CFU/mL) at various acidic pH, and had a low survival rate in Ox gall 0.3% (bile salts). These isolates also showed antibacterial properties against pathogens in the gastrointestinal tract. Both of these bacteria are quite safe to be used together with ofloxacin, linezolid, moxifloxacin, and levofloxacin, antibiotic for tuberculosis therapy.

Conclusions

The results showed that Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk respectively fulfilled the characteristics of probiotic and could potentially be used as adjunct therapy in tuberculosis drug-resistance.


Corresponding author: Budi Suprapti, Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia, Phone: +628155086694, E-mail:

Acknowledgment

We would like to thank the Microbiology Laboratory, Department of Biology, Faculty of Science and Technology, Airlangga University for providing Lactobacillus acidophilus (BioLA246) and Lactobacillus reuteri (BioLR321) isolates for this research. Gratitude are due to The Ministry of Research Technology and Higher Education (KEMRISTEKDIKTI), Indonesia Endowment Fund for Education (LPDP), and BUDI-DN Scholarship.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: Not applicable.

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Received: 2020-11-29
Accepted: 2021-02-25
Published Online: 2021-06-25

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