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Licensed Unlicensed Requires Authentication Published by De Gruyter October 15, 2013

Chemical and pharmacological standardization of Ashwagandhadi lehyam: an ayurvedic formulation

  • Arun Rasheed EMAIL logo , K.V. Satyanarayana , P. Swetha Gulabi and M. Sindhura Rao


Standardization is an important measurement for ensuring the quality control of herbal drugs. It has become essential to develop reliable, specific and sensitive quality control methods. One of the prime ingredients of Ashwagandhadi lehyam is Withania somnifera L. Dunal (Solanaceae) (ashwagandha). In the present study, Ashwagandhadi lehyam was formulated and the quality assessment of the formulation was based on phytochemical screening and physico-chemical evaluation. Ashwagandhadi lehyam was formulated according to a working formula and subjected to phytochemical screening by FTIR analysis and HPTLC fingerprinting, heavy metal determination by AAS, determination of alcohol content, tested for Escherichia coli, Staphylococcus aureus, aerobic bacteria, yeasts and mould, oral toxicity studies and anti-epileptic activity by MES method. The physico-chemical studies showed total ash content as 6.45%, extractive values and some trace elements such as lead, mercury, cadmium and arsenic with 3.2, 0.05, 0.18 and 0.48 ppm, respectively. FTIR and HPTLC studies revealed the presence of functional groups of withanolides in Ashwagandhadi lehyam, resulting in its chemical standardization. The formulation exhibited less epileptic seizures in various phases when compared with that of standard phenytoin and found to possess better anti-inflammatory activity, thus making it biologically standardized. The physico-chemical and pharmacological analysis to standardize Ashwagandhadi lehyam confirmed its use as a safe anti-inflammatory agent and for various seizure disorders.


1. RasheedA, SatyanarayanaKV, Swetha GulabiP, SindhuraM, Neelufar ShamaS, Avinash KumarRG. Standardization: accessing key for herbal drug safety assurance. J Pharm Res2011;4:13225.Search in Google Scholar

2. GuptaM, BishtD, PandeyMM, OjhaSK, KhatoonS, RastogiS, et al. Standardization of Ashwagandhadi lehya – an important Ayurvedic formulation of Withania sominifera. Ind J Trad Know2011;10:5948.Search in Google Scholar

3. MukharjeePK. Quality control of herbal drugs-An approach to evaluation of botanicals. New Delhi, India: Business Horizons Pharmaceutical Publications, 2002.Search in Google Scholar

4. Ayurvedic formulary of India Part-1. 2nd revised Ed. Ministry of health and family welfare. New Delhi, India: Government of India, 2003:10813.Search in Google Scholar

5. GuptaMK, SharmaPK. Text book of pharmacognosy. Meerut, India: Pragati Prakashan Limited, 2007.Search in Google Scholar

6. NadkarniKM. Indian materia medica. Bombay, India: Popular Prakashan Limited, 1976.Search in Google Scholar

7. MohammadHM, MoyanoE, BonfillM, CusidoRM, PalazonJ. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules2009;14:237393.10.3390/molecules14072373Search in Google Scholar PubMed PubMed Central

8. World Health Organization. Quality control methods for medicinal plant materials. Geneva: WHO, 2007.Search in Google Scholar

9. Indian Pharmacopoeia. Ministry of health and family welfare. New Delhi, India: Government of India, 1996.Search in Google Scholar

10. HarborneJB. Phytochemical methods – a guide to modern techniques of plant analysis. New York: Chapman and Hall, 1984.Search in Google Scholar

11. WagnerH, BladtS. Plant drug analysis: a thin layerchromatography atlas. Berlin, Heidelberg: Springer-Verlag, 1996.10.1007/978-3-642-00574-9Search in Google Scholar

12. KalaiselvanV, ShahAK, PatelFB, ShahCN, KalaivaniM, RajasekharanA. Quality assessment of different marketed brands of Dasamoolaristam, an Ayurvedic formulation. Int J Ayur Res2010;1:1013.10.4103/0974-7788.59937Search in Google Scholar PubMed PubMed Central

13. ChattopadhyayRN, ChattopadhyayR, RoyS, MoitraSK. A simple method for plethysmometric measurement of paw volume of small laboratory animals in evaluation of anti inflammatory effects. Bull Calcutta School Trop Med1986;34:58.Search in Google Scholar

14. SwinyardEA. Laboratory evaluation of antiepileptic drugs. Epilepsia1969;10:10719.10.1111/j.1528-1157.1969.tb03838.xSearch in Google Scholar PubMed

Received: 2012-02-15
Accepted: 2013-09-15
Published Online: 2013-10-15

©2013 by Walter de Gruyter Berlin / Boston

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