Abstract
Background: The effects of Orchis anatolica plant roots on adult male mice testicular structure and function were explored and compared with those of the red Korean Panax ginseng.
Methods: Both plants were administered orally to two separate mice groups at a dose of 800 mg/kg/day for 35 days and compared with control group. After treatment, 5 mice of each group were sacrificed and total mice weights, reproductive organs’ weights, spermatogenesis, and androgenic serum markers were investigated. The remaining mice from all groups were allowed to mate with virgin female mice to explore male fertility potential.
Results: Results indicated that body and organs’ weights were increased significantly in mice treated with Orchis anatolica where only slight changes were observed in red Korean Panax ginseng treated and control mice. Intestinal testicular Leydig cells and germinal cells counts were elevated significantly in mice treated with Orchis anatolica plant when compared with the other two groups. Testicular dynamics parameters recorded in both testes and caudae epididymides were found to be increased only in Orchis anatolica treated mice. Although testosterone serum titer was observed to be elevated in both treated mice groups a marked elevation was observed only in Orchis anatolica treated mice. Follicular stimulating hormone blood serum titer was also elevated obtained in Orchis anatolica treated mice when compared with the other two groups. The fertility index of male mice treated with Orchis anatolica plant was enhanced to a greater value when allowed mating with virgin female mice if compared with male mice fertility index recorded in the other two groups.
Conclusions: We can conclude that Orchis anatolica plant could be more useful to improve testicular functions and structural parameters in mice when compared with red Korean Panax ginseng.
References
1. CraggGM, NewmanDJ, SnaderKM. Natural products in drug discovery and development. J Nat Prod1997;60:52–60.10.1021/np9604893Search in Google Scholar
2. BulpittCJ, LiY, BulpittPF, WangJ. The use of orchids in Chinese medicine. R Soc Med2007;100:558–63.10.1177/0141076807100012014Search in Google Scholar
3. HossainMM. Therapeutic orchids: traditional uses and recent advances – an overview. Fitoterapia2011;82:102–40.10.1016/j.fitote.2010.09.007Search in Google Scholar
4. HewCS, ArdittiJ, LinWS. Three orchids used as herbal medicines in China: an attempt to reconcile Chinese and Western pharmacology. In: ArdittJ, PridgeonAM, editors. Orchid biology: reviews and perspectives. Dordrecht, Netherlands: Kluwer Academic, 1997:VII, 213–83.Search in Google Scholar
5. MaridassM, Zahir HussainMI, RajuG. Phytochemical survey of orchids in the Tirunelveli hills of South India. Ethnobot Leaflets2008;12:705–12.Search in Google Scholar
6. AllouhMZ, KhouriNA, DaradkaHM, KaddumiEG. Orchis anatolica root ingestion improves sexual motivation and performance in male rats. J Complement Integr Med2010;7:1–12.10.2202/1553-3840.1462Search in Google Scholar
7. KhouriNA, NawasrehM, Al-HussainSM, Al-KofahiAS. Effects of orchids (Orchis anatolica) on reproductive function and fertility in adult male mice. Reprod Med Biol2006;5:269–76.10.1111/j.1447-0578.2006.00152.xSearch in Google Scholar
8. ThakurM, DixitVK. Aphrodisiac activity of Dactylorhiza hatagirea (D. Don) soo in male albino rats. Evid Based Complement Altern Med2007;4 Suppl 3:419–23.10.1093/ecam/nem111Search in Google Scholar
9. KhouriNA, DaradkaH. Histopathological effects of short and long term treatment of Orchis anatolica crude root extract on female albino rats fertility and pregnancy. Pak J Biol Sci2012;15:198–202.10.3923/pjbs.2012.198.202Search in Google Scholar
10. KhouriNA, DaradkaH. Anti-diabetic effect of Orchis anatolica root extracts on alloxan induced diabetic rats. Comp Clin Pathol2013;22:347–54.10.1007/s00580-012-1415-8Search in Google Scholar
11. CiceroAF, BandieriE, ArlettiR. Lepidium meyenii walp improves sexual behaviour in male rats independently from its action on spontaneous locomotor activity. J Ethnopharmacol2001;75:225–9.10.1016/S0378-8741(01)00195-7Search in Google Scholar
12. BarbaraG. Orchis anatolica boiss. Diagn Pl Orient2004;1844:56.Search in Google Scholar
13. DafniA, BernhardtB. Pollination of terrestrial orchids of southern Australia and the Mediterranean region. Evol Biol1990;24:193–252.Search in Google Scholar
14. GravendeelB, SmithsonA, SlikFJ, SchuitemanA. Epiphytism and pollinator specialization: drivers for orchid diversity?Philos Trans R Soc Lond B Biol Sci2004;359:1523–35.10.1098/rstb.2004.1529Search in Google Scholar PubMed PubMed Central
15. KhouriNA, DaradkaHM, AlkofahiAS. Androgenic mediated effects of dried Orchis anatolica plant root bulbs on male rats’ reproductive system. Comp Clin Pathol2013;23:735–43.10.1007/s00580-013-1678-8Search in Google Scholar
16. BaegI-H, SoS-H. The world ginseng market and the ginseng (Korea). J Ginseng Res2013;37:1–7.10.5142/jgr.2013.37.1Search in Google Scholar
17. ChoiKT. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer. Acta Pharmacol Sin2008;29:1109–18.10.1111/j.1745-7254.2008.00869.xSearch in Google Scholar
18. JiaL, ZhaoY, LiangXJ. Current evaluation of the millennium phytomedicine-ginseng (II): collected chemical entities, modern pharmacology, and clinical applications emanated from traditional Chinese medicine. Curr Med Chem2009;16:2924–42.10.2174/092986709788803204Search in Google Scholar
19. JangDJ, MyeongSL, Byung-CheulS, Young-CheoulL. Edzard Ernst red ginseng for treating erectile dysfunction: a systematic review. Br J Clin Pharmacol2008;66:444–50.10.1111/j.1365-2125.2008.03236.xSearch in Google Scholar
20. HwangSY, SohnSH, WeeJJ, YangJB, KyungJS, KwakYS, et al. Panax ginseng improves senile testicular function in rats. J Ginseng Res2010;34:327–35.10.5142/jgr.2010.34.4.327Search in Google Scholar
21. SalvatiG, GenovesiG, MarcelliniL, PaoliniP, De NuccioI, PepeM, et al. Effects of Panax ginseng C.A. Meyer saponins on male fertility. Soc Sci Med2006;63:732–42.Search in Google Scholar
22. ChenX, 2TonyJ-F.Lee Ginsenosides-induced nitric oxide-mediated relaxation of the rabbit corpus cavernosum Brtish Journal of Pharmacology1995;115:15–8.10.1111/j.1476-5381.1995.tb16313.xSearch in Google Scholar
23. LüJ-M, YaoQ, ChenC. Ginseng compounds: an update on their molecular mechanisms and medical applications. Curr Vasc Pharmacol2009;7:293–302.10.2174/157016109788340767Search in Google Scholar
24. BakM-J, KimK-B, JunM, JeongW-S. Safety of red ginseng oil for single oral administration in Sprague–Dawley rats. J Ginseng Res2014;38:78–81.10.1016/j.jgr.2013.11.009Search in Google Scholar
25. WHO. Protocol MB50 A method for examining the effect of plant extracts administration orally on the fertility of male mouses. New York: Popper and Sons, 1983.Search in Google Scholar
26. PrasadMR, ChinoyNJ, ChinoyNJ, KadamKM. Changes in succinate dehydrogenase levels in the mouse epididymis under normal and altered physiological condition. Fertil Steril1972;23:186–90.10.1016/S0015-0282(16)38825-2Search in Google Scholar
27. AberrcrombieM. Estimation of nuclear population from microtome section. Anat Rec1946;94:238–48.10.1002/ar.1090940210Search in Google Scholar PubMed
28. DixonW, MasseyFJ. Introduction of statistical analysis. New York: McGRaw Hill, 1957:228.Search in Google Scholar
29. McCarthyMM. Handbook of neurochemistry and molecular neurobiology: chapter 11 sex differences in neurotransmitters and behavior. New York: Springer, 2007:459–85.Search in Google Scholar
30. O’ShaughnessyPJ, MonteiroA, VerhoevenG, DeGendtK, AbelMH. Effect of FSH on testicular morphology and spermatogenesis in gonadotropin-deficient hypogonadal mice lacking androgen receptors. Reproduction2010;139:177–84.10.1530/REP-09-0377Search in Google Scholar PubMed PubMed Central
31. SnyderEM, SmallCL, LiY, GriswoldMD. Regulation of gene expression by estrogen and testosterone in the proximal mouse reproductive tract. Biol Reprod2009;81:707–16.10.1095/biolreprod.109.079053Search in Google Scholar PubMed PubMed Central
32. ThackrayGV, PamelaL, MellonDC. Hormones in synergy: regulation of the pituitary gonadotropin genes. Mol Cell Endocrinol2010;314:192199.10.1016/j.mce.2009.09.003Search in Google Scholar PubMed PubMed Central
33. AkingbemiTB. Estrogen regulation of testicular function. Reprod Biol Endocrinol2005;3:51–61.10.1186/1477-7827-3-51Search in Google Scholar PubMed PubMed Central
34. SimorangkirDR, RamaswamyS, MarshallGR, PohlCR, PlantTM. A selective monotropic elevation of FSH, but not that of LH, amplifies the proliferation and differentiation of spermatogonia in the adult rhesus monkey (Macaca mulatta). Hum Reprod2009;24:584–1595.10.1093/humrep/dep052Search in Google Scholar PubMed PubMed Central
35. WuD, GoreAC. Changes in androgen receptor, estrogen receptor alpha, and sexual behavior with aging and testosterone in male rats. Horm Behav2010;58:306–16.10.1016/j.yhbeh.2010.03.001Search in Google Scholar PubMed PubMed Central
36. WalkerWH, ChengJ. FSH and testosterone signaling in Sertoli cells. Reproduction2005;130:15–28.10.1530/rep.1.00358Search in Google Scholar PubMed
37. McLachlanRI, WrefordNG, O’DonnellL, de KretserDM, RobertsonDM. The endocrine regulation of spermatogenesis: independent roles for testosterone and FSH. J Endocrinol1996;148:1–9.10.1677/joe.0.1480001Search in Google Scholar PubMed
38. WangRS, YehS, ChenLM, LinHY, ZhangC, NiJ, et al. Androgen receptor in Sertoli cell inessentials for germ cell nursery and junctional complex formation in mouse testes. Endocrinology2006;147:5624–33.10.1210/en.2006-0138Search in Google Scholar PubMed
39. HoldcraftRW, BraunRE. Androgen receptor function is required in Sertoli cells for the terminal function is required in Sertoli cells for the terminal differentiation of haploid spermatids. Development2004;131:459–67.10.1242/dev.00957Search in Google Scholar PubMed
40. TsaiMY, YehSD, WangRS, YehS, ZhangC, LinHY, et al. Differential effects of spermatogenesis and fertility in mice lacking androgen receptor in individual testis cells. Proc Natl Acad Sci USA2006;103:18975–80.10.1073/pnas.0608565103Search in Google Scholar PubMed PubMed Central
41. DierichA, SairamMR, MonacoL, FimiaGM, GansmullerA, LeMeurM, et al. Impairing follicle-stimulating hormone (FSH) signaling in vivo: targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance. PNAS1998;95:13612–17.10.1073/pnas.95.23.13612Search in Google Scholar PubMed PubMed Central
42. De KretserDM. Is spermatogenic damage associated with Leydig cell dysfunction?J Clin Endocrinol Metab2004;89:3158–60.10.1210/jc.2004-0741Search in Google Scholar PubMed
43. GriffinDK, EllisPJ, DunmoreB, BauerJ, AbelMH, AffaraNA. Transcriptional profiling of luteinizing hormone receptor-deficient mice before and after testosterone treatment provides insight into the hormonal control of postnatal testicular development and cell differentiation. Biol Reprod2010;82:1139–50.10.1095/biolreprod.109.082099Search in Google Scholar PubMed PubMed Central
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