Accessible Requires Authentication Published by De Gruyter July 24, 2014

In vitro anti-hyperglycemic and antioxidant properties of extracts from the stem bark of Ceiba pentandra

Christian Kuete Fofie, Sylvie Léa Wansi, Elvine Pami Nguelefack-Mbuyo, Albert Donatien Atsamo, Pierre Watcho, Albert Kamanyi, Tsabang Nole and Télesphore Benoît Nguelefack

Abstract

Background: The goal of the study was to determine the antidiabetic mechanisms and the antioxidant effects of aqueous (decoction and maceration) and methanol extracts from the stem bark of Ceiba pentandra.

Methods: These extracts were tested in vitro on glucose uptake by skeletal muscles and liver slices and on glucose release by liver slices. The antioxidant activities of C. pentandra extracts were investigated at concentrations ranging from 1 to 300 µg/mL on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2)-induced hemolysis, H2O2-induced brain lipid peroxidation, hydroxyl (˙OH) radical as well as their reducing power.

Results: The decoction similarly to insulin exhibited a significant glucose lowering activity. In a hyperglycemic milieu, it significantly increased glucose uptake by the liver by 56.57% and in the skeletal muscle by 94.19%. In a hypoglycemic milieu, it significantly reduced glucose release by the liver by 33.94%. The decoction, maceration and methanol extracts exhibited a significant radical scavenging activity on DPPH with respective EC50 of 87.84, 54.77 and 6.15 µg/mL versus 2.24 µg/mL observed with ascorbic acid. All the extracts showed a significant antioxidant effect on hydroxyl radical, against lipid peroxidation and H2O2-induced hemolysis. The decoction showed the greatest antihemolytic effect with a maximum inhibition of 77.57% at the concentration of 100 µg/mL. C. pentandra extracts also showed a concentration-dependent reducing power.

Conclusions: These results suggest that the antidiabetic effect of C. pentandra is due to its ability to increase glucose uptake and to reduce glucose release by target organs. The antioxidant properties of C. pentandra extracts are additional benefit for their antidiabetic effects.

Acknowledgments

The authors are grateful to the International Foundation for Sciences (IFS) for the grant N° F-4576-1 offered to Pr Télesphore Benoît Nguelefack.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2014-5-17
Accepted: 2014-7-6
Published Online: 2014-7-24
Published in Print: 2014-9-1

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