Background: Conventional plant-based therapies act as an important therapeutic tool for the treatment of worm infections all over the world and continuous evaluation of medicinal plants to find new potential lead compounds should be carried out.
Methods: In-vitro analysis was conducted to evaluate the probable anthelmintic effect of crude aqueous and hydroalcoholic extracts of Ananas sativus leaves, Erythrina variegata barks and Alocasia indica rootstocks, against adult Paramphistomum cervi (Trematoda) and Haemonchus contortus (Nematode).
Results: Among all three concentrations (25, 50, and 100 mg/mL), the hydroalcoholic leaf extract of A. sativus exhibited paralysis and death time ranged between 7.26 to 26.76 min and 15.40 to 35.55 min respectively for P. cervi while that for H. contortus was 14.70 to 42.43 min and 23.43 to 56.34 min, respectively. Moreover, aqueous extract exhibited paralysis and death time ranged between 7.66 to 28.72 min and 18.30 to 33.00 min, respectively, for P. cervi whereas paralysis and death time ranged between 23.34 to 37.88 min and 31.08 to 58.30 min respectively for H. contortus. Both extracts of E. variegata bark and A. indica tuber showed comparatively less significant anthelmintic activity. All results were statistically significant (p<0.001).
Conclusions: A. sativus leaf displayed favorable anthelmintic activity on both P. cervi and H. contortus, whereas E. variegata barks and A. indica rootstocks showed insignificant result.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
1. KellyGS. Bromelain: A literature review and discussion of its therapeutic applications. Altern Med Rev1996;1:243–57.Search in Google Scholar
2. ThomsonAB, KeelanM, ThiesenA, ClandininMT, RopeleskiM, WildGE. Small bowel review normal physiology. Part 1. Digestive Dis Sci2001;46:2567–87.Search in Google Scholar
3. HaleLP, GreerPK, TrinhCT, GottfriedMR. Treatment with oral Bromelain decreases colonic inflammation in the IL-10-deficient murine model of inflammatory bowel disease. Clin Immunol2005;116:135–42.10.1016/j.clim.2005.04.011Search in Google Scholar
4. RahmanMZ, RahmanMS, KaiserA, HossainA, RashidMA. Bioactive isoflavones from Erythrina variegata L. Turk J Pharm Sci2010;7:21–8.Search in Google Scholar
5. WarrierPK, NambiarVP, AnonymousRC. Indian medicinal plants a compendium of 500 species. 1st ed. Hyderabad: Orient Longman Limited, 1994.Search in Google Scholar
6. GuptaVK. Anonymous. The wealth of India (A dictionary of Indian raw materials and industrial product). 3rd ed. New Delhi: National Institute of Science Communication and Council of Industrial and Scientific Research, 2002.Search in Google Scholar
7. PrajapatiND. Handbook of medicinal plants. Jodhpur: Agrobios India, 2003:32.Search in Google Scholar
8. MullaA, PrafullS, AjinkyaP, HarshadT, FahimS. Evaluation of antimicrobial activity of leaves of Alocasia indica Schott. Int J Pharm Technol Res2010;2:327–33.Search in Google Scholar
9. MullaA, VaradS, PatilR, BuradeK. Anthelmintic activity of leaves of Alocasia indica Schott. Int J Pharm Technol Res2010;2:26–30.Search in Google Scholar
10. TandonV, PalP, RoyB, RaoHSP, ReddyKS. In vitro anthelmintic activity of root-tuber extract of Flemingia vestita, an indigenous plant in Shillong, India. Parasitol Res1997;83:492–8.10.1007/s004360050286Search in Google Scholar
11. GotoC, KasuyaS, KogaK, OhtomoH, KagelN. Lethal efficacy of extract from zingiber officinale (traditional Chinese medicine) or -shogaol and -gingerol in anisakis larvae in vitro. Parasitol Res1990;76:653–6.10.1007/BF00931082Search in Google Scholar
12. AthanasiadouS, KyriazakisI, JacksonF, CoopRL. Direct anthelmintic effects of condensed tannins towards different gastrointestinal nematodes of sheep: in vitro and in vivo studies. Vet Parasitol2001;99:205–19.10.1016/S0304-4017(01)00467-8Search in Google Scholar
13. AkhtarMS, IqbalZ, KhanMN, LateefM. Anthelmintic activity of medicinal plants with particular reference to their use in animals in the Indo-Pakistan subcontinent. S Rumin Res2000;38:99–107.10.1016/S0921-4488(00)00163-2Search in Google Scholar
©2015 by De Gruyter