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Licensed Unlicensed Requires Authentication Published by De Gruyter April 7, 2015

The effect of Terminalia chebula extract vs. silver sulfadiazine on burn wounds in rats

  • Ebrahim Nasiri , Seyed Jalal Hosseinimehr EMAIL logo , Mohammad Azadbakht , Jafar Akbari , Reza Enayati-fard and Sohail Azizi

Abstract

Background: The aim of this study was to evaluate the efficacy of ethanol extract of Terminalia chebula in the treatment of hot water-induced burn wounds and to compare the results with silver sulfadiazine (SSD) in rats.

Methods: Five groups of ten rats were burned with hot water. Animals were administrated with topical cream at control, normal saline, 5 %, 10 % T. chebula extracts and standard SSD creams. Several parameters were assessed, including wound area, visual inspection of wound, bacteriology assessment, percentage of wound contraction and histopathological examination.

Results: The average area of wound on 10th day was 11.2 ± 3.3, 11 ± 3.2, 8.5 ± 2.3, 9.5 ± 4.2 and 5.1 ± 2.2 cm2 for SSD1 %, base cream, normal saline, herbal extract 5 % and 10 %, respectively. T. chebula 10 % cream exhibited lower wound size than SSD1 % and control groups at 10th day post burn injury. On day 15, the percentage of wound contraction in T. chebula was 5 % and 10 % more than 18 % and 53 % in comparison to SSD1 % group, respectively. Histopathological results exhibited well-formed horizontally oriented collagen fibers for appropriate tension. Strength of scar on burned tissue was better in T. chebula 5 % and 10 % as compared with control and SSD groups.

Conclusions: The results of this study showed that T. chebula extract was capable of accelerating wound healing in rats by wound contraction, and had beneficial effects more than SSD 1 % cream in the management of burn injury.

Acknowledgments

This study was supported by a grant from Mazandaran University of Medical Sciences, Sari, Iran. This research was the subject of a PhD thesis of Ebrahim Nasiri, a student of Mazandaran University of Medical Sciences.

Author contributions

All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.Research funding: None declared.Employment or leadership: None declared.Honorarium: None declared.Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

[1] UpadhyayN, KumarR, MandotraS, MeenaR, SiddiquiM, SawhneyR, et al. Safety and healing efficacy of sea buckthorn Hippophae rhamnoides seed oil on burn wounds in rats. Food Chem Toxic2009;47(6):114653.10.1016/j.fct.2009.02.002Search in Google Scholar

[2] KhorasaniGA, HosseinimehrSJ, AzadbkhtM, ZamaniA, MahdaviMR. Aloe versus silver sulfadiazine creams for second-degree burns: a randomized controlled study. Surg Today2009;39:58791.10.1007/s00595-008-3944-ySearch in Google Scholar

[3] PriyaKS, GnanamaniA, RadhakrishnanN, BabuM. Healing potential of Datura alba on burn wounds in albino rats. J Ethnopharmacol2002;83(3):19399.10.1016/S0378-8741(02)00195-2Search in Google Scholar

[4] HosseinimehrSJ, KhorasaniG, AzadbakhtM, ZamaniP, GhasemiM, AhmadiA. Effect of aloe cream versus silver sulfadiazine for healing burn wounds in rats. Acta Dermatovenerol Croat2010;18(1):27.Search in Google Scholar

[5] PereiraT, Dos SantosD, Lima-RibeiroMHM, De Pontes-FilhoNT, Carneiro-LeãoAMdA, CorreiaMTDSDevelopment of animal model for studying deep second-degree thermal burns. Biomed Res Int2012;46084:17.10.1155/2012/460841Search in Google Scholar PubMed PubMed Central

[6] HassanzadehG, HajmamouchehriF, Beheshti roiA, HassanzadehN, ShafighN, BarzroudipourM, et al. Comparing effects of silver sulfadiazine, sucrafate and brassica oleracea extract on burn wound healing. Life Sci J2013;10(6s):85255.Search in Google Scholar

[7] SingerAJ, ClarkRAF. Cutaneous wound healing. N Engl J Med1999;341:73846.10.1056/NEJM199909023411006Search in Google Scholar PubMed

[8] VelnarT, BaileyT, The WoundSV. Healing process: an overview of the cellular and molecular mechanisms. J Int Med Res2009;37:152842.10.1177/147323000903700531Search in Google Scholar PubMed

[9] LiHL, ChenLP, HuYH, QinY, LiangG, XiongYX, et al. Crocodile oil enhances cutaneous burn wound healing and reduces scar formation in rats. Acad Emerg Med2012;19(3):26573.10.1111/j.1553-2712.2012.01300.xSearch in Google Scholar PubMed

[10] EpsteinFH, SingerAJ, ClarkRA. Cutaneous wound healing. N Engl J Med1999;341(10):73846.10.1056/NEJM199909023411006Search in Google Scholar

[11] MoenadjatY, SetiabudyR, DalimaAW, AstrawinataGS. The safety and efficacy of feracrylum as compared to silver sulfadiazine in the management of deep partial thickness burn: a clinical study report. Med J Indones2008;17:25971.10.13181/mji.v17i4.329Search in Google Scholar

[12] FullerFW. The side effects of silver sulfadiazine. J Burn Care Res2009;30(30):46470 (Abstract).10.1097/BCR.0b013e3181a28c9bSearch in Google Scholar PubMed

[13] HermansM. Results of a survey on the use of different treatment options for partial and full thickness burns. Burns1998;24(6):53951.10.1016/S0305-4179(98)00079-5Search in Google Scholar

[14] GuptaPC. Biological and pharmacological properties of Terminalia chebula Retz (haritaki), an overview. Int J Pharm Pharm Sci2012;4(3):6268.Search in Google Scholar

[15] GurungS, Škalko-BasnetN. Wound healing properties of Carica papaya latex: in vivo evaluation in mice burn model. J Ethnopharmacol2009;121(2):33841.10.1016/j.jep.2008.10.030Search in Google Scholar

[16] SüntarIP, AkkolEK, YılmazerD, BaykalT, KırmızıbekmezH, AlperM, et al. Investigations on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol2010;127(2):46877.10.1016/j.jep.2009.10.011Search in Google Scholar

[17] AkhoondinasabMR, AkhoondinasabM, SaberiM, IranpourM. Assessing effect of three herbal medicines in second and third degree burns in rats and comparison with silver sulfadiazine ointment. Burns2015;41(1):12531.10.1016/j.burns.2014.04.001Search in Google Scholar

[18] ChengH-Y, LinT-C, YuK-H, YangC-M, LinC-C. Antioxidant and free radical scavenging activities of Terminalia chebula. Biol Pharm Bull2003;26(9):133135.10.1248/bpb.26.1331Search in Google Scholar

[19] NaikG, PriyadarsiniK, NaikD, GangabhagirathiR, MohanH. Studies on the aqueous extract of Terminalia chebula as a potent antioxidant and a probable radioprotector. Phytomedicine2004;11(6):53038.10.1016/j.phymed.2003.08.001Search in Google Scholar

[20] MalekzadehF, EhsanifarH, ShahamatM, LevinM, ColwellRR. Antibacterial activity of black myrobalan (Terminalia chebula Retz) against Helicobacter pylori. Int J Antimicrob Agents2001;18:8588.10.1016/S0924-8579(01)00352-1Search in Google Scholar

[21] VinodN, SurenderS, YogendraKG. Anti-arthritic and disease modifying activity of Terminalia chebula Retz, in experimental models. JPP2010;62:18016.10.1111/j.2042-7158.2010.01193.xSearch in Google Scholar PubMed

[22] GuptaD, BhaskarDJ, GuptaRK, KarimB, GuptaV, PuniaH, et al. Effect of Terminalia chebula extract and chlorhexidine on salivary pH and periodontal health: 2 weeks randomized control trial. Phytother Res2014;28:99298.10.1002/ptr.5075Search in Google Scholar PubMed

[23] MomenSM. Tohfatol-momenin (Tohfehe-Hakim Momen). Tehran: Traditional Medicine of Research Center of Shahid Beheshti University of Medical Sciences, Nashre Shar Constitiute, 2008.Search in Google Scholar

[24] UpadhyayA, AgrahariP, SinghDK. A review on the pharmacological aspects of Terminalia chebula. Int J Pharmacol2014;10(6):28998.10.3923/ijp.2014.289.298Search in Google Scholar

[25] KhorasaniGA, HosseinimehrSJ, ZamaniP, GhasemiM, AhmadiA. The effect of saffron (Crocus sativus)extract for healing of second-degree burn wound in rats. Keio J Med2008;57(4):19095.10.2302/kjm.57.190Search in Google Scholar

[26] de OliveiraAP, de Souza FrancoE, Rodrigues BarretoR, CordeiroDP, de MeloRG, de AquinoCMF, et al. Effect of semisolid formulation of Persea americana Mill (avocado) oil on wound healing in rats. Evid Based Complement Alternat Med. 2013;472382:18.10.1155/2013/472382Search in Google Scholar

[27] KhooYT, HalimAS, SinghKKB, MohamadNA. Wound contraction effects and antibacterial properties of tualang honey on full-thickness burn wounds in rats in comparison to hydrofibre. BMC Complement Alternat Med2010;10(48):1048.10.1186/1472-6882-10-48Search in Google Scholar

[28] HaghdoostF, Baradaran MahdaviMM, ZandifarA, SaneiMH, ZolfaghariB, JavanmardSH. Pistacia atlantica resin has a dose-dependent effect on angiogenesis and skin burn wound healing in rat. Evid Based Complement Alternat Med2013;893425:18.10.1155/2013/893425Search in Google Scholar

[29] EdrakiM, AkbarzadehA, HosseinzadehM, TanidehN, SalehiA, HealingK-HO. Effect of sea buckthorn, olive oil, and their mixture on full-thickness burn wounds. Adv Skin Wound Care2014;27(7):31723.10.1097/01.ASW.0000451061.85540.f9Search in Google Scholar

[30] LonchinS, SurjeetS, PitchumaniS, PadmavathiS, GowriC. Influence of Terminalia chebula on dermal wound healing in rats. Phytother Res2002;16:22731.10.1002/ptr.827Search in Google Scholar

[31] ElizabetKM. Antimicrobial activity of Terminalia chebula on Pseudomonas aeruginosa from nasal secretions of chronic sinus patient. J Hum Ecol2002;13(4):31114.10.1080/09709274.2002.11905554Search in Google Scholar

[32] KannanP, RamadeviS. Antibacterial activity of Terminalia chebula fruit extract. Afr J Microbiol Res2009;3(4):18084.Search in Google Scholar

[33] AnejaKR, RadhikaJ. Evaluation of antimicrobial properties of fruit extracts of Terminalia chebula against dental caries pathogens. Jundishapur J Microbiol2009;2(3):10511.Search in Google Scholar

[34] LiK, DiaoY, ZhangH, WangS, ZhangZ, YuB, et al. Tannin extracts from immature fruits of Terminalia chebula fructus Retz. Promote cutaneous wound healing in rats. BMC Complement Alternat Med2011;86(11):19.10.1186/1472-6882-11-86Search in Google Scholar

[35] SaleemA, HusheemM, HarkonenP, PihlajaK. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula Retz. Fruit J Ethnopharmacol2002;81:32736.10.1016/S0378-8741(02)00099-5Search in Google Scholar

[36] GhoshPK, GabaA. Phyto-extracts in wound healing. J Pharm Pharm Sci2013;16(5):760820.10.18433/J3831VSearch in Google Scholar PubMed

[37] BansoA, AdeyemoSA. Evaluation of antibacterial properties of tannins isolated from Dichrostachys cinerea. Afr J Biotechnol2007;6(15):178587.10.5897/AJB2007.000-2262Search in Google Scholar

[38] SenCK, KhannaS, GordilloG, BagchiD, BagchiM, OxygenSR. Oxidants, and antioxidants in wound healing. Ann N Y Acad Sci2002;957(1):23949.10.1111/j.1749-6632.2002.tb02920.xSearch in Google Scholar PubMed

[39] KuntalD. Wound healing potential of aqueous crude extract of Stevia rebaudiana in mice. Rev Bras Farmacogn2013;23(2):35157.10.1590/S0102-695X2013005000011Search in Google Scholar

[40] MohajeriG, MasoudpourH, HeidarpourM, KhademiEF, GhafghaziS, AdibiS, et al. The effect of dressing with fresh kiwifruit on burn wound healing. Surg2010;148(5):96368.10.1016/j.surg.2010.02.013Search in Google Scholar PubMed

Received: 2014-11-13
Accepted: 2015-1-15
Published Online: 2015-4-7
Published in Print: 2015-6-1

©2015 by De Gruyter

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