Reactive oxygen species (ROS), products of normal cell metabolism may cause damage to biological macromolecules leading to severe health threats when they are present in high concentrations. Aromatic plants contain phytochemicals rich of antioxidants that prevent oxidant formation or scavenge oxidants produced under oxidative stress conditions. In the present study, we investigated the free radical scavenging effects, the antioxidant and ion toxicity preventive effect of Xylopia aethiopica (X. aethiopica), a plant of the family of Annonaceae used as spice in Cameroon. The scavenging properties of extracts of X. aethiopica were tested on 2, 2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), hydroxyl (OH), 2,2′-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals. The total antioxidant capacity was assayed by ferric reducing ability of plasma (FRAP), phosphomolybdenum antioxidant power (PAP), reduction assays. The protective potential was carried on superoxide dismutase (SOD), catalase and peroxidases. The results showed that both the ethanolic (BEE) and the hydroethanolic (BEH) extracts from the barks of X. aethiopica scavenged all the tested radicals. The sample BEH showed the highest total antioxidant capacity both in the FRAP and the PAP. This result was positively correlated to its higher phenolic content (30.74±0.44 CAE/g dried extract). The higher protective capacity of BEH on SOD, catalase and peroxidase activities was comparable to that of the vitamin C used as standard. In conclusion, X. aethiopica has a higher antioxidant and protective potential against ion-mediated oxidative damage and may be considered as a potential drug against metal-mediated toxicity.
The authors are grateful to Mr Nana from the National Herbarium for helping in collecting and identifying the plant material. The authors acknowledge the Department of Chemical Engineering Materials Environment of Sapienza University of Rome for their hospitality and access to scientific instrumentation.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. Moukette MB conducted the study and prepared the manuscript; Biapa NPC conducted the statistical analysis; Pieme CA designed the research and co-directed the research work with Ngogang YJ and provided reagents. All the authors read and approved the final manuscript.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report or in the decision to submit the report for publication.
1. OmoruyiB, BradleyG, AfolayanA.Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province. BMC Complement Altern Med2012;12:215.10.1186/1472-6882-12-215Search in Google Scholar PubMed PubMed Central
2. WangD, WangL, ZhuF, ZhuJ, ChenX, ZouL.In vitro and in vivo studies on the antioxidant activities of the aqueous extracts of Douchi (a traditional Chinese salt-fermented soybean food). Food Chem2008;107:1421–8.10.1016/j.foodchem.2007.09.072Search in Google Scholar
3. De MarcoE, SavareseM, PaduanoA, SacchiR.Characterization and fractionation of phenolic compounds extracted from olive oil mill wastewaters. Food Chem2007; 104:858–67.10.1016/j.foodchem.2006.10.005Search in Google Scholar
4. NanfackP, BiapaN, PiemeC, MoorVA, MouketteB, NgogangY.The in vitro antisickling and antioxidant effects of aqueous extracts Zanthoxyllum heitzii on sickle cell disorder. BMC Complement Altern Med2013;13:162.10.1186/1472-6882-13-162Search in Google Scholar PubMed PubMed Central
5. PiemeC, NgoupayoJ, NkoulouC, MouketteB, NonoB, MoorV, et al. Syzygium guineense extracts show antioxidant activities and beneficial activities on oxidative stress induced by ferric chloride in the liver homogenate. Antioxidants2014;3:1–18.10.3390/antiox3030618Search in Google Scholar PubMed PubMed Central
6. PiemeC, PenlapV, NkegoumB, TaziebouI, NgogangJ.In vivo antioxidant and potential antitumor activity extract of Leea guineensis Royen ex. L. (Leeaceae) on carcinomatous cells. Pharmacologyonline2008;1:538–47.Search in Google Scholar
7. PiemeC, PenlapV, EtoaF.Screening of plants for antimicrobial and antitumor activity. Western African Network of natural products research scientist (WANNPRES). Afr J Tradit Complement Altern Med2005;2:117–205.Search in Google Scholar
8. BiapaN, ObenJ, NgogangJ.Scavenging radical kinetic and antianaemic screening properties of some medicinal plants used in Cameroon. Int J Appl Res Nat Prod2011;4:29–35.Search in Google Scholar
9. BiapaP, MateiH, BâliciS, ObenJ, NgogangJ.Protective effects of stem bark of Harungana madagascariensis on the red blood cell membrane. BMC Complement Altern Med2013;13:1–9.10.1186/1472-6882-13-98Search in Google Scholar PubMed PubMed Central
10. Bakarnga-ViaI, HzoundaJ, FokouP, TchokouahaL, Gary-BoboM, GalludA, et al. Composition and cytotoxic activity of essential oils from Xylopia aethiopica (Dunal) A. Rich, Xylopia parviflora (A. Rich) Benth. and Monodora myristica (Gaertn) growing in Chad and Cameroon. BMC Complement Altern Med2014;14:125.10.1186/1472-6882-14-125Search in Google Scholar PubMed PubMed Central
11. AdaramoyeO, SarkarJ, SinghN, MeenaS, ChangkijaB, YadavP, et al. Antiproliferative action of Xylopia aethiopica fruit extract on human cervical cancer cells. Phytother Res2011;25:1558–63.10.1002/ptr.3551Search in Google Scholar PubMed
12. ChoumessiA, DanelM, ChassaingS, TruchetI, PenlapV, PiemeA, et al. Characterization of the antiproliferative activity of Xylopia aethiopica. Cell Div2012;7:8.10.1186/1747-1028-7-8Search in Google Scholar PubMed PubMed Central
15. ReR, PellegriniN, ProteggenteA, PannalaA, YangM, Rice-EvansC.Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med1999;26:1231–7.10.1016/S0891-5849(98)00315-3Search in Google Scholar
19. PrietoP, PinedaM, AguilarM.Spectrophotometric quantitative of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem1999;269:337–41.10.1006/abio.1999.4019Search in Google Scholar
21. SingletonV, RossiJ.Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic1965;16:144–58.Search in Google Scholar
22. ZhishenJ, MengchengT, JianmingW.The determination of flavonoid contents in mulberry and their scavenging effects on superoxide. Radicals Food Chem1999;64:555–9.10.1016/S0308-8146(98)00102-2Search in Google Scholar
24. KumarS, MishraA, PandeyA.Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models. BMC Complement Altern Med2013;13:120.10.1186/1472-6882-13-120Search in Google Scholar PubMed PubMed Central
25. GuptaV, SharmaM.Protective effect of Cinnamomum tejpata on lipid peroxide formation in isolated rat liver homogenate. Curr Res J Biol Sci2010;2:246–9.Search in Google Scholar
28. DongmoF, ObenE, MomoN, MandobE.Phytochemical constituents and antioxidant potential of some Cameroonian medicinal plants. Pharmacologyonline2007;2:436–52.Search in Google Scholar
30. ThilagamE, KumarappanC, ParimaladeviB, MandalS.Antioxidant Activity of Senna surattensis leaves. Int J Adv Pharm Biol Chem2012;1:454–60.Search in Google Scholar
32. HertogM, FeskensE, HollmanP, KatanM, KromhoutD.Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet1993;342:1007–11.10.1016/0140-6736(93)92876-USearch in Google Scholar
33. MouketteMB, PiemePC, BiapaNP, NjimouJ, NgogangYJ.Free radicals quenching potential, protective properties against oxidative mediated ion toxicity and HPLC phenolic profile of a Cameroonian spice: Piper guineensis. Toxicol Rep2015. DOI:10.1016/j.toxrep.2015.02.007.10.1016/j.toxrep.2015.02.007Search in Google Scholar PubMed PubMed Central
34. MukherjeeS, PawarN, KulkarniO, NagarkarB, ThopteS, BhujbalA, et al. Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana. BMC Complement Altern Med2011;11:38.10.1186/1472-6882-11-38Search in Google Scholar PubMed PubMed Central
35. SreedharV, NathL, GopalN, NathM.In-vitro antioxidant activity and free radical scavenging potential of roots of Vitex trifoliate. Res J Pharm Biol Chem Sci2010;1:1044–36.Search in Google Scholar
36. RosaLD, Alvarez-ParillaE, Gonzalez-AguilarG.Fruit and vegetables phytochemicals: chemistry, nutritional value and stability. Ames, IA: Blackwell Publishing,2010.Search in Google Scholar
37. MouketteMB, PiemePC, NjimouJ, BiapaNP, MarcoB, NgogangYJ.In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Biol Res2015. DOI:10.1186/s40659-015-0003-1.10.1186/s40659-015-0003-1Search in Google Scholar PubMed PubMed Central
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