Abstract
Background: Traditional remedies employ herbal drugs for the treatment of liver ailments and hepatoprotection. Thus, the present study was designed to evaluate the hepatoprotective effect of “extract of Anacyclus pyrethrum Linn” (APE) against antitubercular drug-induced hepatotoxicity in rats.
Methods: Group I rats (normal control) received vehicle (1 % CMC), while group II rats (hepatotoxic control) isoniazid (INH) plus rifampicin (RIF) each 50 mg/kg/day po, for 28 days. Group III, IV and V rats were administered with APE 200, APE 400 and silymarin 100 mg/kg/day po, respectively, for 28 days. Concurrently, hepatotoxicity was tried to induce by coadministration of INH and RIF each 50 mg/kg/day po for 28 days in group III, IV and V rats. After 24 h of the last dosing, blood was obtained under light anesthesia and the rats were killed. Hepatoprotective effect was assessed by liver weight, relative liver weight and biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), serum bilirubin, cholesterol, total protein and albumin levels.
Results: Group IV rats showed significant (p<0.01) decrease in SGPT, SGOT, ALP, LDH, cholesterol, serum bilirubin, liver weight and relative liver weight Levels, while significant (p<0.01) increase in final body weight (b. wt.), total protein and albumin levels as compared to group II rats. Hepatoprotective effect of APE 400 mg/kg/day was comparable to that of silymarin 100 mg/kg/day and the hepatic marker levels were also restored. Hepatoprotective effect of APE was well supported by the histopathological results.
Conclusions: Hydroalcoholic APE root possesses hepatoprotective activity as it exhibited the protective effect against INH plus RIF-induced hepatotoxicity in rats.
Acknowledgments
The authors are thankful to the Faculty of Pharmacy, Integral University, for providing all the necessary facilities related to the present research work.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Jalayer NN, Niakan M, Khodadadi E. Determination of antimicrobial activity of Anacyclus pyrethrum extract against some of the oral bacteria: An in vitro study. J Dent Shiraz Univ Med Sci 2012;13(2):59–63.Search in Google Scholar
2. Puri HS. Rasayana: Ayurvedic herbs for longevity and rejuvenation. London: Taylor and Francis, 2003:71–3.Search in Google Scholar
3. Selles C, AmineDib ME, Djabou N, Beddou F, Muselli A, Tabti B, et al. Antimicrobial activity and evolution of the composition of essential oil from Algerian Anacyclus pyrethrum L. through the vegetative cycle. Nat Prod Res 2013;27(23):2231–4.10.1080/14786419.2013.811409Search in Google Scholar
4. Doudach L, Meddah B, Alnamer R, Chibani F, Cherrah Y. In vitro antibacterial activity of the methanolic and aqueous extracts of Anacyclus pyrethrum used in Moroccan traditional medicine. Int J Pharm Pharm Sci 2012;4(3):402–5.Search in Google Scholar
5. Dalila B, Korrichi L, Dalila S. Immunologically active polysaccharides isolated from Anacyclus pyrethrum. Libyan Agric Res Cen J Intl 2010;1(3):128–33.Search in Google Scholar
6. Rimbau V, Risco E, Canigueral S, Iglesias J. Antiinflammatory activity of some extracts from plants used in the traditional medicine of north-African countries. Phytother Res 1996;10(5):421–3.10.1002/(SICI)1099-1573(199608)10:5<421::AID-PTR851>3.0.CO;2-USearch in Google Scholar
7. Zaidi SMA, Pathan SA, Singh S, Jamil S, Ahmad FJ, Khar RK. Anticonvulsant, anxiolytic and neurotoxicity profile of Aqarqarha (Anacyclus pyrethrum) DC (compositae) root ethanolic extract. Pharmacol Pharm 2013;4:535–41.10.4236/pp.2013.47077Search in Google Scholar
8. Sharma V, Thakur M, Chauhan NS, Dixit VK. Evaluation of the anabolic, aphrodisiac and reproductive activity of Anacyclus pyrethrum DC in male rats. Sci Pharm 2009;77:97–110.10.3797/scipharm.0808-14Search in Google Scholar
9. Tyagi S, Mansoori MH, Singh NK, Shivhare MK, Bhardwaj P, Singh RK. Antidiabetic effect of Anacyclus pyrethrum DC in alloxan induced diabetic rats. Eur J Biol Sci 2011;3(4):117–20.Search in Google Scholar
10. Kalim MD, Bhattacharyya D, Banerjee A, Oxidative CS. DNA damage preventive activity and antioxidant potential of plants used in Unani system of medicine. BMC Complement Altern Med 2010;10:77–87.10.1186/1472-6882-10-77Search in Google Scholar
11. Selles C, AmineDib ME, Allali H, Tabti B. Evaluation of antimicrobial and antioxidant activities of solvent extracts of Anacyclus pyrethrum L., from Algeria. Mediterranean J Chem 2012;2(2):408–15.10.13171/mjc.2.2.2012.01.11.22Search in Google Scholar
12. Sujith K, Darwin R, Suba V. Toxicological evaluation of ethanolic extract of Anacyclus pyrethrum in albino Wistar rats. Asian Pac J Trop Dis 2012;2(6):437–41.10.1016/S2222-1808(12)60096-6Search in Google Scholar
13. Hanane E, Aminata S, Fatima E, Amar B, Mohamed A, Belghiti TZ. Phytochemical study of Anacyclus pyrethrum (L.) of Middle Atlas (Morocco), and in vitro study of antibacterial activity of pyrethrum. Adv Nat Appl Sci 2014;8(8):131–40.Search in Google Scholar
14. Navarro VJ, Senior JR. Drug-related hepatotoxicity. N Engl J Med 2006;354:731–9.10.1056/NEJMra052270Search in Google Scholar
15. Abideen PS. Implementation of self reporting pharmacovigilance in anti tubercular therapy using knowledge based approach. J Pharmacovigil 2013;1(1):1–5.Search in Google Scholar
16. Tasduq SA, Peerzada K, Koul S, Bhat R, Johri RK. Biochemical manifestations of antituberculosis drugs induced hepatotoxicity and the effect of silymarin. Hepatol Res 2005;31(3):132–5.10.1016/j.hepres.2005.01.005Search in Google Scholar
17. Jehangir A, Nagi AH, Shahzad M, Azam Z. The hepato-protective effect of Cassia fistula (Amaltas) leaves in isoniazid and rifampicin induced hepatotoxicity in rodents. Biomedica 2010;26(1):25–9.Search in Google Scholar
18. Pundir R, Singh G, Pandey AA, Saraf SA. Demand of herbal hepatoprotective formulations in Lucknow – A survey. Pharm Res 2009;1:23–33.Search in Google Scholar
19. Bafna PA, Balaraman R. Effect of activit, a herbomineral formulation, on experimentally induced gastric lesions in rats. J Appl Pharm Sci 2011;1(10):134–9.Search in Google Scholar
20. Banskota AH, Tezuka Y, Adnyam IK, Ishi E, Midrikawa K, Matsuhsige K, et al. Helicobacter pylori activities of constituents from Brazilian propolis. Phytomedicine 2001;8:16–23.10.1078/0944-7113-00004Search in Google Scholar
21. Rahman MA, Hussain A. Anticancer activity and apoptosis inducing effect of methanolic extract of Cordia dichotoma against human cancer cell line. Bangladesh J Pharmacol 2015;10:27–34.10.3329/bjp.v10i1.20883Search in Google Scholar
22. Mujahid M, Siddiqui HH, Hussain A, Hussain S. Hepatoprotective effects of Adenanthera pavonina against anti-tubercular drugs-induced hepatotoxicity in rats. Pharmacogn J 2013;5:286–90.10.1016/j.phcgj.2013.08.003Search in Google Scholar
23. Belur B, Kandaswamy N, Mukherjee KL. Medical laboratory technology – A procedure manual for routine diagnostic tests. New Delhi: Tata McGraw Hill Co. Ltd., Laboratory Techniques in Histopathology, 1990:1124–88.Search in Google Scholar
24. Yong L, Jing Z, Peiyu X, Yimei W, Jun Z, Li J, et al. Protective effects of metallothionein on isoniazid and rifampicin-induced hepatotoxicity in mice. PloS ONE 2013;8(8):1–8.Search in Google Scholar
25. Santhosh S, Sini TK, Anandan R, Mathee PT. Hepatoprotective activity of chitosan against isoniazid and rifampicin-induced toxicity in experimental rats. Eur J Pharmacol 2007;572:69–73.10.1016/j.ejphar.2007.05.059Search in Google Scholar
26. Zhang ZH, Tang JH, Zhan ZL, Zhang XL, Wu HH. Cellular toxicity of isoniazid together with rifampicin and the metabolites of isoniazid on QSG-7701 hepatocytes. Asian Pac J Trop Med 2012;5:306–9.10.1016/S1995-7645(12)60044-3Search in Google Scholar
27. Chen X, Xu J, Zhang C, Yu T, Wang H. The protective effects of ursodeoxycholic acid on isoniazid plus rifampicin induced liver injury in mice. Eur J Pharmacol 2011;659:53–60.10.1016/j.ejphar.2011.03.007Search in Google Scholar PubMed
28. Georgieva N, Gadjeva V, Tolekova A. New isonicotinoylhydrazones with SSA protect against oxidative-hepatic injury of isoniazid. Trakia J Sci 2004;2(1):37–43.Search in Google Scholar
29. Kale BP, Kothekar MA, Tayade HP, Jaju JB. Effect of aqueous extracts of Azadirachta indica leaves on hepatotoxicity induced by anti-tubercular drugs in rats. Indian J Pharmacol 2003;35:177–80.Search in Google Scholar
30. Kumar VK, Lalitha KG. Acute oral toxicity studies of Anacyclus pyrethrum DC root in albino rats. Int J Pharm Pharm Sci 2013;5(4):675–8.Search in Google Scholar
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