Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 25, 2016

Influence of piperine and quercetin on antidiabetic potential of curcumin

  • Ginpreet Kaur EMAIL logo , Mihir Invally and Meena Chintamaneni


Background: Curcumin is a nutraceutical obtained from the rhizomes of Curcuma longa with a significant medicinal value against numerous disorders. However, the potential cannot be completely exploited due to low in vivo bioavailability. Hence, in order to enhance the bioavailability of curcumin, we combined it with the bioavailability enhancers like piperine and quercetin.

Methods: The present study was targeted to explore the antidiabetic potential of combinatorial extract of curcumin with piperine and quercetin (CPQ) in streptozotocin- and nicotinamide-induced diabetic rats. Diabetes mellitus was induced by single intraperitoneal injection of streptozotocin (55 mg/kg) and nicotinamide (120 mg/kg–1). CPQ was orally administered at 100 mg kg–1 dose/day for a period of 28 days. At the end of 28 days, blood was analyzed for glucose, high density lipoprotein (HDL), low density lipoprotein (LDL) and total cholesterol level. Oral glucose tolerance test (OGTT) was also conducted at the end of 28 days.

Results: Oral administration of CPQ at the dose of 100 mg kg–1 significantly (p<0.01) reduced plasma glucose at the end of 28 days, as compared to the diabetic control group. The reduction in the plasma glucose produced by the CPQ extract was equivalent to that of glibenclamide and significantly more compared to curcumin alone (p<0.01). Furthermore, a significant (p<0.01) reduction in the raised LDL, cholesterol and triglycerides and improvement was observed in the group fed with CPQ compared to diabetic control as well as the alone (p<0.05) curcumin group. There was a significant improvement in the body weight with CPQ compared to diabetes control group. OGTT revealed a significantly high glucose tolerance in CPQ fed rats compared to the diabetic control as well as the rats fed with curcumin alone.

Conclusions: Treatment with combinatorial extract of curcumin presented a significantly better therapeutic potential when compared with curcumin alone, which reveals that CPQ, with reduced dose of curcumin may serve as a therapeutic agent in the treatment of type 2 diabetes mellitus.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Kitabchi A, Umpierrez G, Miles J, Fisher J. Hyperglycemic crises in adult patients with diabetes. Diabetes Care 2009;32:1335–43.10.2337/dc09-9032Search in Google Scholar

2. Patlak M. New weapons to combat an ancient disease: treating diabetes. FASEB J 2002;16:1853–7.10.1096/fj.02-0974bktSearch in Google Scholar

3. Waugh N, Cummins E, Royle P, Clar C, Marien M, Richter B, et al. Newer agents for blood glucose control in type 2 diabetes: systematic review and economic evaluation. Health Technol Assess 2010;14:12–48.10.3310/hta14360Search in Google Scholar

4. Mallery LH, Ransom T, Steeves B, Cook B, Dunbar P, Moorhouse P. Evidence-informed guidelines for treating frail older adults with type 2 diabetes: from the Diabetes Care program of Nova Scotia (DCPNS) and the Palliative and Therapeutic Harmonization (PATH) program. J Am Med Dir Assoc 2013;14:801–8.10.1016/j.jamda.2013.08.002Search in Google Scholar

5. International Diabetes Federation. Managing older people with type 2 diabetes: global guideline [Internet. Brussels: IDF, 2013. Available at: Accessed: 12 Nov 2015.Search in Google Scholar

6. Azevedo M, Alla S. Diabetes in sub-Saharan Africa: Kenya, Mali, Mozambique, Nigeria, South Africa and Zambia. Int J Diabetes Dev Ctries 2008;28:101–8.10.4103/0973-3930.45268Search in Google Scholar

7. Global burden of diabetes. International diabetes federation. Diabetic atlas fifth edition 2011, Brussels. Available at: Accessed: 18 Oct 2015.Search in Google Scholar

8. Ripsin CM, Kang H, Urban RJ. Management of blood glucose in type 2 diabetes mellitus. Am Fam Physician 2009;79:30–6.Search in Google Scholar

9. Olokoba AB, Obateru OA, Olokoba LB. Type 2 diabetes mellitus: a review of current trends. Oman Med J 2012;27:269–73.10.5001/omj.2012.68Search in Google Scholar

10. Dahanukar S, Kulkarni R, Rege N. Pharmacology of medicinal plants and natural products. Indian J Pharmacol 2000;32:S81–S118.Search in Google Scholar

11. Arora RB, Basu N, Kapoor V. Anti-inflammatory studies on Curcuma longa (turmeric). Indian J Med Res 1971;59:1289–305.Search in Google Scholar

12. Kiso Y, Suzuki Y, Watanabe N, Oshima Y, Hikino H. Antihepatotoxic principles of Curcuma longa rhizomes. Planta Med 1983;49:185–7.10.1055/s-2007-969845Search in Google Scholar

13. Ruby AJ, Kuttan G, Babu KD, Rajasekharan KN, Kuttan R. Anti-tumour and antioxidant activity of natural curcuminoids. Cancer Lett 1995;94:79–83.10.1016/0304-3835(95)03827-JSearch in Google Scholar

14. Srivastava R, Dikshit M, Srimal RC, Dhawan BN. Antithrombotic effect of curcumin. Thromb Res 1985;40:413–17.10.1016/0049-3848(85)90276-2Search in Google Scholar

15. Srinivasan K. Role of spices beyond food flavouring: nutraceuticals with multiple health effects. Food Rev Int 2005;2:167–88.10.1081/FRI-200051872Search in Google Scholar

16. Sharma RA, Euden SA, Platton SL, Cooke DN, Shafayat A. Phase I clinical trial of oral curcumin: biomarkers of systemic activity and compliance. Clin Cancer Res 2004;10:6847–54.10.1158/1078-0432.CCR-04-0744Search in Google Scholar

17. Shoba G, Joy D, Joseph T. Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers. Planta Med 1998;64:353–6.10.1055/s-2006-957450Search in Google Scholar

18. Ginpreet K, Meena C. Evaluation of antihyperlipidemic activity of a combinatorial extract of curcumin, piperine and quercetin in triton-induced hyperlipidemia in rats. Sci Int 2013;1:57–63.10.5567/sciintl.2013.57.63Search in Google Scholar

19. Ginpreet K, Meena C. Amelioration of obesity, glucose intolerance, and oxidative stress in high-fat diet and low-dose streptozotocin-induced diabetic Rats by combination consisting of “curcumin with piperine and quercetin,” ISRN Pharmacology 2012; 2012: Article ID 957283, 7 pages. doi:10.5402/2012/957283Search in Google Scholar

20. Astha M, Ginpreet K, Piperine MC. Quercetin enhances antioxidant and hepatoprotective effect of curcumin in paracetamol induced oxidative stress. Int J Pharmacol 2012;8:101–7.10.3923/ijp.2012.101.107Search in Google Scholar

21. Khandelwal KR. Practical pharmacognosy techniques and experiments, 14th ed. Pune (India): Nirali Prakashan, 2005.Search in Google Scholar

22. Kokate CK. Practical pharmacognosy, 4th ed. New Delhi: Vallabh Prakashan, 1997.Search in Google Scholar

23. OECD. 2001. Acute oral toxicity test method. In: OECD Guidelines for testing of chemicals, No. 423. Organization for economic cooperation and development, Paris, France.Search in Google Scholar

24. Shirwaikar A, Rajendran K, Punitha I. Antidiabetic activity of alcoholic stem extract of Coscinium fenestratum in streptozotocin-nicotinamide induced type 2 diabetic rats. J Ethnopharmacol. 2005;97:369–74.10.1016/j.jep.2004.11.034Search in Google Scholar

25. Patel A, Ginpreet K, Meena C. α-Glucosidase inhibitory activity of Curcumin and its comparison with combinatorial extract consisting of curcumin with piperine and quercetin. Pharmacology online 2011;3:796–801.Search in Google Scholar

26. Suryanarayana P, Satyanarayana A, Balakrishna N, Kumar PU, Reddy GB. Effect of turmeric and curcumin on oxidative stress and antioxidant enzymes in streptozotocin-induced diabetic rats. Med Sci Monit 2007;13:BR 286–302.Search in Google Scholar

27. Tokaç M, Taner G, Aydın S, Ozkardeş AB, Dündar HZ, Taşlıpınar MY, et al. Protective effects of curcumin against oxidative stress parameters and DNA damage in the livers and kidneys of rats with biliary obstruction. Food Chem Toxicol 2013;61:28–35.10.1016/j.fct.2013.01.015Search in Google Scholar

28. Kumar S, Sharma S, Vasudeva N. Screening of antidiabetic and antihyperlipidemic potential of oil from Piper longue and piperine with their possible mechanism. Expert Open Pharmacother 2013;14:1723–36.10.1517/14656566.2013.815725Search in Google Scholar

29. Jadhav R. Hypoglycaemic and antidiabetic activity of flavonoids: bowelled acid, pelagic acid, quercetin, ruin on streptozotocin-nicotinamide induced type 2 diabetic rats. Int J Pharm Pharm Sci 2002;4:251–6.Search in Google Scholar

30. Chen D, Wang MW. Development and application of rodent models for type 2 diabetes. Diabetes, Obesity Metab 2005;7:307–17.10.1111/j.1463-1326.2004.00392.xSearch in Google Scholar

31. Caspary WF. Sucrose malabsorption in man after ingestion of alpha-glucosidehydrolase inhibitor. Lancet 1978;1:1231–3.10.1016/S0140-6736(78)92466-2Search in Google Scholar

Received: 2016-3-5
Accepted: 2016-5-19
Published Online: 2016-6-25
Published in Print: 2016-9-1

©2016 by De Gruyter

Downloaded on 4.3.2024 from
Scroll to top button