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Evaluation of the sub-chronic toxicity profile of the corm of Xanthosoma sagittifolium on hematology and biochemistry of alloxan-induced diabetic Wistar rats

  • Olayinka A. Oridupa EMAIL logo , Oluyemisi F. Folasire and Adedotun J. Owolabi



Hematological and biochemical changes associated with diabetes mellitus and probable reversal were assessed in alloxan-induced diabetic Wistar rats fed with varied percentages of Xanthosoma sagittifolium corm feed (Xs). The changes were compared to normoglycemic rats and diabetic rats treated with glibenclamide.


The study had eight groups in all with group 8 (control) consisting of five normoglycemic rats fed with normal rat pellets (Nrp). Diabetes was experimentally induced by intraperitoneal injection of alloxan to normoglycemic rats. Diabetic rats (serum glucose >200 mg/dL) at 48 h postinjection were randomly divided into the seven groups, each diabetic group consisting of five rats. One group was untreated and fed with Nrp, four groups were fed with 25 %, 50 %, 75 % or 100 % Xs, one group was fed with 100 % Xs and administered with glibenclamide, while a 7th group was fed with Nrp and administered with glibenclamide.


This study shows that treatment of diabetes with corm of X. sagittifolium increases cellular response to inflammation which is required for body defense against assaulting agents. Decreased serum protein levels observed in untreated diabetic rats were restored in diabetic rats fed with X. sagittifolium corm with particular increase in serum albumin levels but depression of globulin fraction, except in rats fed with X. sagittifolium feed and administered with glibenclamide. X. sagittifolium showed a potent antihyperglycemic effect and corrected the dyslipidemia in a manner comparable to that observed for glibenclamide. Although HDL levels were still low, significant (p<0.05) decrease of LDL levels was a positive indicator of reduced risk for development of cardiovascular and/or coronary heart disease.


X. sagittifolium corm can be recommended for inclusion in diets of diabetics without causing further deterioration of health of the diabetic patients.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2008;31:S62–S67.10.2337/dc09-S062Search in Google Scholar PubMed PubMed Central

2. Giugliano D, Marfella R, Coppola L, Verrazzo G, Acampora R, Giunta R, et al. Vascular effects of acute hyperglycemia in humans are reversed by l-arginine: evidence for reduced availability of nitric oxide during hyperglycemia. Circulation. 1997;95:1783–1790.10.1161/01.CIR.95.7.1783Search in Google Scholar PubMed

3. Capes SE, Hunt D, Malmberg K, Pathak P, Gerstein HC. Stress hyperglycemia and prognosis of stroke in nondiabetic and diabetic patients: a systematic overview. Stroke. 2001;32:2426–2432.10.1161/hs1001.096194Search in Google Scholar PubMed

4. Funnell MM, Brown TL, Childs BP, Haas LB, Hosey GM, Jensen B, et al. National standards for diabetes self-management education. Diabetes Care. 2009;32:S87–S94.10.2337/dc09-S087Search in Google Scholar PubMed PubMed Central

5. Kitabchi AE, Umpierrez GE, Miles JM, Fisher JN. Hyperglycemic crises in adult patients with diabetes. Diabetes Care. 2009;32:1335–1343.10.2337/dc09-9032Search in Google Scholar PubMed PubMed Central

6. Shukia R, Sharma SB, Puri D, Prabhu KM, Murthy PS. Medicinal plants for treatment of diabetes mellitus. Indian J Clin Biochem. 2000;15:169–177.10.1007/BF02867556Search in Google Scholar PubMed PubMed Central

7. Babu PA, Suneetha G, Boddepalli R, Lakshmi VV, Rani TS, et al. A database of 389 medicinal plants for diabetes. Bioinformation. 2006;1:130–131. : As per style, all author names should be listed if there are fewer than or equal to 6 authors. If there are more than 6 authors present, list out the first six authors followed by ellipses with last author name. So please check and provide accordingly for all single author et al. references.10.6026/97320630001130Search in Google Scholar PubMed PubMed Central

8. Ocvirk S, Kistler M, Khan S, Talukder SH, Hauner H. Traditional medicinal plants used for the treatment of diabetes in rural and urban areas of Dhaka, Bangladesh – an ethnobotanical survey. J Ethnobiol Ethnomed. 2013;9:43–50.10.1186/1746-4269-9-43Search in Google Scholar PubMed PubMed Central

9. Echebiri RN. Socio-economic factors and resource allocation in cocoyam production in Abia State, Nigeria: a case study. J Sustainable Trop Agric Res. 2004;9:69–73.Search in Google Scholar

10. Okoye BC, Asumugha GN, Okezie CA, Tanko L, Onyenweaku CE. Econometric assessment of the trend in cocoyam production in Nigeria, 1960/1961 – 2003/2006. Agric J. 2008;3:99–101.Search in Google Scholar

11. Onwueme IC. An analysis of the constraints in the delivery systems for the tropical root and tuber crops in tropical root crops in a developing economy. Proceedings of the ninth symposium. Accra, Ghana1991;52–53.Search in Google Scholar

12. Uguru MI. Crop production: tools, techniques and practice. Nsukka: Fulladu Publishing Company; 1996.Search in Google Scholar

13. Ogunniyi LT. Profit efficiency among cocoyam producers in Osun State, Nigeria. Int J Agric Econ Rural Dev. 2008;1:38–46.Search in Google Scholar

14. Eleje I. Cocoyam, a major national carbohydrate staple for the future in Nigeria. In: Arene OB, Ene LS, Odurukwe SO, Ezeh NO, editors. Proc. 1st national workshop on cocoyams. Umudike, Nigeira: NRCRI; 1987:11–16.Search in Google Scholar

15. Onwueme IC. Strategies for increasing cocoyam (Colocasia and Xanthosoma sp.) in Nigerian food basket. In: Arene OB, Ene LS, Odurukwe SO, Ezeh NO, editors. Proc. 1st national workshop on cocoyam. Umudike, Nigeria: NRCRI; 1987:35–42.Search in Google Scholar

16. Akobundu EN, Hoskins FH. Potential of corn-cowpea mixtures as infant food. J Food Sci Agric. 1987;2:111–114.Search in Google Scholar

17. Oti E, Akobundu EN. Potentials of cocoyam-soybean-crayfish mixtures in complementary feeding. Niger Agric J. 2008;39:137–145.10.4314/naj.v39i1.3279Search in Google Scholar

18. Luna B, Feinglos MN. Drug-induced hyperglycemia. JAMA. 2001;286:1945–1948.10.1001/jama.286.16.1945Search in Google Scholar PubMed

19. Eddouks M, Maghrani M, Lemhadri A, Ouahidi ML, Jouad H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet). J Ethnopharmacol. 2002;82:97–103.10.1016/S0378-8741(02)00164-2Search in Google Scholar

20. Hilmi Y, Abushama MF, Abdalgadir H, Khalid A, Khalid H. A study of antioxidant activity, enzymatic inhibition and in vitro toxicity of selected traditional sudanese plants with anti-diabetic potential. BMC Complement Altern Med. 2013;14–149.10.1186/1472-6882-14-149Search in Google Scholar PubMed PubMed Central

21. Satchell SC, Tooke JE. What is the mechanism of microalbuminuria in diabetes: a role for the glomerular endothelium? Diabetologia. 2008;51–714.10.1007/s00125-008-0961-8Search in Google Scholar PubMed PubMed Central

22. Palmiere C. Postmortem diagnosis of diabetes mellitus and its complications. Croat Med J. 2015;56:181–193.10.3325/cmj.2015.56.181Search in Google Scholar PubMed PubMed Central

23. Devasagayam HK, Ashodi D, Natesan S. Evaluation of anti-diabetic activity of ethanolic and aqueous extracts of stem and leaves of Alangium salvifolium and Pavonia zeylanica. Asian Pac J Trop Biomed. 2012;1:1–5.Search in Google Scholar

24. Saba AB, Rahman SA, Oridupa OA. Antidiabetic and hypolipidemic effects of Moringa oleifera ethanolic leaf extract on experimentally-induced diabetes in Wistar rats. J Nat Sci Eng Tech. 2012;11:32–41.Search in Google Scholar

25. Alba-Loureiro TC, Munhoz CD, Martins JO, Cerchiaro GA, Scavone C, Curi R, et al. Neutrophil function and metabolism in individuals with diabetes mellitus. Braz J Med Biol Res. 2007;40:1037–1044.10.1590/S0100-879X2006005000143Search in Google Scholar

26. Saba AB, Oridupa OA, Oyagbemi AA, Alao EO. Serum biochemical changes accompanying prolonged administration of ethanolic extract of whole fruit of Lagenaria breviflora (Benth) Roberty in Wistar rats. Afr J Biotech. 2010;9:7128–7133.Search in Google Scholar

27. Kolb H, Mandrup-Poulsen T. The global diabetes epidemic as a consequence of life- style-induced low-grade inflammation. Diabetologia. 2010;53:10–20.10.1007/s00125-009-1573-7Search in Google Scholar PubMed

28. Taylor JJ, Preshaw PM, Lalla E. A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes. J Clin Periodontol. 2013;40:S113–S134.10.1111/jcpe.12059Search in Google Scholar PubMed

29. Waikar SS, Bonventre JV. Can we rely on blood urea nitrogen as biomarker to determine when to initiate dialysis? Clin J Am Soc Nephrol. 2010;1:903–904.10.2215/CJN.02560706Search in Google Scholar PubMed

30. Manna P, Das J, Ghosh J, Sil PC. Contribution of type 1 diabetes to rat liver dysfunction and cellular damage via activation of NOS, PARP, IκBα/NF-κB, MAPKs, and mitochondria-dependent pathways: prophylactic role of arjunolic acid. Free Rad Biol Med. 2010;48:1465–1484.10.1016/j.freeradbiomed.2010.02.025Search in Google Scholar PubMed

31. Zhang C, Lu X, Tan Y, Li B, Miao X, Jin L, et al. Diabetes-induced hepatic pathogenic damage, inflammation, oxidative stress, and insulin resistance was exacerbated in zinc deficient mouse model. Plos ONE. 2012;7:49257.10.1371/journal.pone.0049257Search in Google Scholar PubMed PubMed Central

32. Ragavan B, Krishnakumari S. Effect of T. arjuna stem bark extract on histopathology of liver, kidney and pancreas of alloxan-induced diabetic rats. Afr J Biomed Res. 2006;9:189–197.10.4314/ajbr.v9i3.48904Search in Google Scholar

33. Daryoush M, Bahram AT, Yousef D, Mehrdad N. Brassica rapa L. extract alleviate early hepatic injury in alloxan-induced diabetic rats. J Med Plants Res. 2011;5:6813–6821.Search in Google Scholar

34. Barter P, Gotto AM, LaRosa JC, Maroni J, Szarek M, Grundy MS, et al. HDL cholesterol, very low levels of LDL cholesterol, and cardiovascular events. N Engl J Med. 2007;357:1301–1310.10.1056/NEJMoa064278Search in Google Scholar

35. Khera AV, Cuchel M, de la Llera-Moya M, Rodrigues A, Burke MF, Jafri K, et al. Cholesterol efflux capacity, high-density lipoprotein function, and atherosclerosis. New Engl J Med. 2011;364:127–135.10.1056/NEJMoa1001689Search in Google Scholar

36. Saba AB, Oridupa OA. Lipoproteins and cardiovascular diseases. In: Frank S, Kostner G, editors. Lipoproteins – role in health and diseases. Rijeka, Croatia: In-Tech Open Access; 2012:197–222. Chapter 8. Available at in Google Scholar

37. Grundy SM, Paternak R, Greenland P, Smith S, Fuster V. Assessment of cardiovascular risk by use of multiple-risk-factor assessment equations. Circulation. 1999;100:1481–1492.10.1161/01.CIR.100.13.1481Search in Google Scholar

38. Huxley R, Barzi F, Woodward M. Excess risk of fatal coronary heart disease associated with diabetes in men and women: meta-analysis of 37 prospective cohort studies. BMJ. 2006;332:73–78.10.1136/bmj.38678.389583.7CSearch in Google Scholar

39. Carnethon MR, Biggs ML, Barzilay J, Kuller LH, Mozaffarian D, Mukamal K, et al. Diabetes and coronary heart disease as risk factors for mortality in older adults. Am J Med. 2010;123:556.e1–9.10.1016/j.amjmed.2009.11.023Search in Google Scholar

40. Garvey WT, Kwon S, Zheng D, Shaughnessy S, Wallace P, Hutto A, et al. Effects of insulin resistance and type 2 diabetes on lipoprotein subclass particle size and concentration determined by nuclear magnetic resonance. Diabetes. 2003;52:453–462.10.2337/diabetes.52.2.453Search in Google Scholar

41. Grundy SM. Hypertriglyceridemia, insulin resistance, and the metabolic syndrome. Am J Cardiol. 1999;83:25F–29F.10.1016/S0002-9149(99)00211-8Search in Google Scholar

Received: 2016-7-19
Accepted: 2017-1-12
Published Online: 2017-3-11
Published in Print: 2017-3-10

© 2017 Walter de Gruyter GmbH, Berlin/Boston

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