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Licensed Unlicensed Requires Authentication Published by De Gruyter July 22, 2017

Effects of pomegranate peel extract on histopathology, testosterone levels and sperm of testicular torsion–detorsion induced in adult Wistar rats

Mandana Beigi Boroujeni , Said Saied Shahrokhi , Mahdi Birjandi , Abolfazl Abbaszadeh , Fatemeh Beyranvand , Sakineh Hamoleh , Zohreh Zandbaf and Mohammadreza Gholami EMAIL logo



In the present study, effects of pomegranate peel extract have been evaluated on decreasing the damage induced by testis torsion.


In this study, 30 adult Wistar rats were randomly divided into three groups of control, experimental (1) and experimental (2). Control: no ischemia, received vehicle alone, exposed to sham operation. Experimental (1): Received the vehicle alone during ischemia followed by 60 days’ reperfusion. Experimental (2): After performing ischemia reperfusion, 500 mg/kg of pomegranate peel extract has been used for 60 days. Blood samples and sperm samples were collected. Testes were harvested and stained with haematoxylin and eosin to study the structure of seminiferous tubules.


The statistical comparison between sperm count and their viability and testosterone hormone amount showed a significant difference between control and experimental (1) groups and control and experimental (2) groups. The results showed an improvement of morphological condition of seminiferous tubules.


Pomegranate peel extract has revealed desirable changes on the effective parameters in infertility.


This study was approved in the Lorestan University of Medical Sciences. The authors thank the head and staff of Razi Herbal Medicines Research Center of Lorestan Medical University. Special thanks to Lorestan University of Medical Sciences for the financial support, Khorramabad, Iran.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report or in the decision to submit the report for publication.

  3. Research funding: None declared.

  4. Employment or leadership: None declared.

  5. Honorarium: None declared.


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Received: 2017-1-24
Accepted: 2017-4-23
Published Online: 2017-7-22

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