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Accessible Unlicensed Requires Authentication Published by De Gruyter November 9, 2019

Antihyperglycaemic, haemorheological and antioxidant activities of Lychnis chalcedonica L. extract in a streptozotocin-induced rat model of diabetes mellitus

Mark B. Plotnikov, Larisa N. Zibareva, Aleksander S. Vasil’ev, Oleg I. Aliev, Anna M. Anishchenko and Mikhail Yu. Maslov

Abstract

Background

New therapeutic strategies, such as the use of agents to correct rheological disorders, are needed for the prevention and treatment of angiopathy in diabetic patients. The aim of this work was to study the antihyperglycaemic, haemorheologic and antioxidant activities of an extract from the flowering plant Lychnis chalcedonica L. (ELC) and 20-hydroxyecdysone using the streptozotocin-induced model of diabetic rats.

Methods

The streptozotocin-induced model of diabetes was produced using streptozotocin at a dose of 50 mg/kg (ip). Animals from the experimental groups were treated with ELC (150 mg/kg) or 20-hydroxyecdysone (1.1 mg/kg) intragastrically in 1% aqueous starch mucilage daily, for 14 days; rats of control groups received an equal volume of starch mucilage. The following parameters were measured: glucose concentration (GC) in blood, whole blood viscosity (WBV), conjugated dienes in RBC membranes. Macro- and microrheological indicators (viz. plasma viscosity, haematocrit, RBC aggregation (T1/2) and the RBC elongation index (EI)) were additionally measured in rats that received ELC, and in the control group.

Results

After treatment with ELC, the GC in rats was 19% lower than that in the control group (14.7 ± 0.9 mM compared to 18.2 ± 1.1 mM). Rats with streptozotocin-induced diabetes have hyperviscosity syndrome, which is characterized by increased WBV, increased RBC aggregation and decreased deformability. ELC treatment reduced WBV at shear rates of 10–90 s−1 by 5–8%, and T1/2 and EI in the experimental group were 31% and 5–10% higher compared to the control group. 20-Hydroxyecdysone decreased WBV at shear rates of 10–90 s−1 by 3–11%. Finally, ELC and 20-hydroxyecdysone lowered the content of conjugated dienes by 27% and by 26% compared to the control groups.

Conclusion

In the streptozotocin-induced diabetic rat model, ELC showed measurable antihyperglycaemic activity; ELC and 20-hydroxyecdysone demonstrated similar haemorheological, and antioxidant activities.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The authors declare that there is no conflict of interest.

References

[1] Vekasi J, Marton ZS, Kesmarky G, Cser A, Russai R, Horvath B. Hemorheological alterations in patients with diabetic retinopathy. Clin Hemorheol Microcirc 2001;24:59–64.Search in Google Scholar

[2] Le Devehat C, Khodabandehlou T, Vimeux M. Impaired hemorheological properties in diabetic patients with lower limb arterial ischaemia. Clin Hemorheol Microcirc 2001;25:43–8.Search in Google Scholar

[3] Negrean V, Suciu I, Sâmpelean D, Cozma A. Rheological changes in diabetic microangiopathy. Rom J Intern Med 2004;42:407–13.Search in Google Scholar

[4] Le Devehat C, Vimeux M, Bondoux G, Kholndabandehlou T. Red blood cell aggregation in diabetic mellitus. Int Angiol 1990;9:11–15.Search in Google Scholar

[5] Le Devehat C, Kholndabandehlou T, Vimeux M. Relationship between hemorheological and microcirculatory abnormalities in diabetes mellitus. Diabet Metab 1994;20:401–4.Search in Google Scholar

[6] Kotov SV, Kalinin AP, Rudakova IG. Diabetic neuropathy. Moscow: Meditsina, 2000:232 (in Russian).Search in Google Scholar

[7] Solerte SB, Fioravanti M, Cerutti N, Severgnini S, Locatelli M, Pezza N, et al. Retrospective analysis of hemorheologic effects of pentoxifylline in diabetic patients with angiopathic complications. Acta Diabetol 1997;34:67–74.Search in Google Scholar

[8] Vereshchagin EI, Sobolevskaia KA, Yakubova AI. Useful Plants of West Siberia. Moscow-Leningrad: Nauka, 1959:347 (in Russian).Search in Google Scholar

[9] Plotnikov MB, Aliev OI, Vasil’ev AS, Maslov MYu, Zibareva LN, Dmitruk SE, et al. Hemorheological effects of Lychnis chalcedonica L. extract in experimental hypertension in rats. Eksp Klin Farmakol 2000;63:54–6 (in Russian).Search in Google Scholar

[10] Plotnikov MB, Aliev OI, Vasil’ev AS, Maslov MYu, Suslov NI, Zibareva LN. Hemorheological and cerebroprotective activity of Lychnis chalcedonica L. extract in rats with cerebral ischemia. Bull Exp Biol Med 2005;139:60–3.Search in Google Scholar

[11] Lafont R, Dinan L. Practical uses for ecdysteroids in mammals including humans: and update. J Insect Sci 2003;3:1–30.Search in Google Scholar

[12] Zibareva LN, Baltaev UA, Revina TA, Abubakirov NK. Phytoecdysteroids of genus Lychnis plants. Khimiia prirodnykh soedinenii 1991;4:584–5 (in Russian).Search in Google Scholar

[13] Zibareva LN, Saatov Z, Abubakirov NK, Stachisterone D. Viticosterone Е and α-ecdysone from Lychnis chalcedonica. Khimiia prirodnykh soedinenii 1991;4:585–6 (in Russian).Search in Google Scholar

[14] Smolyakova IM, Avdeenko SN, Kalinkina GI, Yusubov MS, Zibareva LN. The study of the chemical composition Lychnis chalcedonica, cultivated in Western Siberia. II. HPLC-chromatographic study of phenolic compounds and ecdysteroids Lychnis chalcedonica, cultivated in Western Siberia. Khimiia rastitel’nogo syr’ia 2010;3:95–102 (in Russian).Search in Google Scholar

[15] Shin S, Hou JX, Suh JS, Singh M. Validation and application of a microfluidic ektacytometer (RheoScan-D) in measuring erythrocyte deformability. Clin Hemorheol Microcirc 2007;37:319–28.Search in Google Scholar

[16] Stoltz JE, Donner M. New trends in clinical hemorheology: an introduction to the concept of the hemorheological profile. Schweiz Med Wochenschr 1991;43:41–9.Search in Google Scholar

[17] Tappel AL. Protection against free radical lipid peroxidation reactions. Adv Exp Med Biol 1978;97:111–31.Search in Google Scholar

[18] Dodge JT, Mitchell C, Hanahan DJ. The preparation and chemical characteristics of hemoglobin-free ghost of human erythrocytes. Arch Biochem Biophys 1963;100:119–30.Search in Google Scholar

[19] Imai S, Toyosato T, Sakai M, Sato Y, Fujioka S. Screening results of plants for phytoecdysones. Chem Pharm Bull (Tokyo) 1969;17:335–9.Search in Google Scholar

[20] Zibareva L, Volodin V, Saatov Z, Savchenko T, Whiting P, Lafont R, et al. Distribution of phytoecdysteroids in the Caryophyllaceae. Phytochemistry 2003;64:499–517.Search in Google Scholar

[21] Zibareva LN, Baltaev UA, Sviridova TP, Saatov Z, Abubakirov NK. Species of the genus Lychnis L. is a promising source of ecdysteroids. Rastitel'nye Resursy 1995;31:1–9 (in Russian).Search in Google Scholar

[22] Matsuda H, Kawaba T, Yamamoto Y. Pharmacological studies of insect metamorphosing steroids from Achyranthis radix. Nippon Yakurigaku Zasshi 1970;66:551–63.Search in Google Scholar

[23] Yoshida T, Otaka T, Uchiyama M, Ogawa S. Effect of ecdysterone on hyperglycemia in experimental animals. Biochem Pharmacol 1971;20:3263–8.Search in Google Scholar

[24] Syrov VN, Iuldasheva NKh, Égamova FR, Ismailova GI, Abdullaev ND, Khushbaktova ZA. Estimation of the hypoglycemic effect of phytoecdysteroids. Eksp Klin Farmakol 2012;75:28–31 (in Russian).Search in Google Scholar

[25] Kosovsky MI, Syrov VN, Mirakhmedov MM, Katkova SP, Khushbatkova ZA. The effect of nerobol and ecdysterone on processes related to insulin regulatory function in normal and in experimental insulin resistance. Probl Endokrinol 1989;35:77–81 (in Russian).Search in Google Scholar

[26] Islam MN, Ishita IJ, Jung HA, Choi JS. Vicenin 2 isolated from Artemisia capillaris exhibited potent anti-glycation properties. Food Chem Toxicol 2014;69:55–62.Search in Google Scholar

[27] Ku SK, Bae JS. Vicenin-2 and scolymoside inhibit high-glucose-induced vascular inflammation in vitro and in vivo. Can J Physiol Pharmacol 2016;94:287–95.Search in Google Scholar

[28] Boudjelal A, Siracusa L, Henchiri C, Sarri M, Abderrahim B, Baali F, et al. Antidiabetic effects of aqueous infusions of Atemisia herba-alba and Ajuga iva in alloxan-induced diabetic rats. Planta Med 2015;81:696–704.Search in Google Scholar

[29] Panda S, Kar A. Apigenin (4',5,7-trihydroxyflavone) regulates hyperglycaemia, thyroid dysfunction and lipid peroxidation in alloxan-induced diabetic mice. J Pharm Pharmacol 2007;59:1543–8.Search in Google Scholar

[30] Rauter AP, Martins A, Borges C, Mota-Filipe H, Pinto R, Sepodes B, et al. Antihyperglycaemic and protective effects of flavonoids on streptozotocin-induced diabetic rats. Phytother Res 2010;24:S133–8.Search in Google Scholar

[31] Babu N, Singh M. Influence of hyperglycemia on aggregation, deformability and shape parameters of erythrocytes. Clin Hemorheol Microcirc 2004;31:273–80.Search in Google Scholar

[32] Attali JR, Valensi P. Diabetes and hemorheology. Diabetes Metab 1990;16:1–6 (in French).Search in Google Scholar

[33] Singh M, Shin S. Changes in erythrocyte aggregation and deformability in diabetes mellitus: a brief review. Indian J Exp Biol 2009;47:7–15.Search in Google Scholar

[34] Tsukada K, Sekizuka E, Oshio C, Minamitani H. Direct measurement of erythrocyte deformability in diabetes mellitus with a transparent microchannel capillary model and high-speed video camera system. Microvasc Res 2001;61:231–9.Search in Google Scholar

[35] Comu FM, Ozturk L, Alkan M, Pampal K, Arslan M, Isik B, et al. Investigation of the effects of propofol and vitamin C administration on erythrocyte deformability in rats with streptozotocin-induced diabetes mellitus. Bratisl Lek Listy 2014;115:400–4.Search in Google Scholar

[36] Spasov AA, Samokhina MP, Bulanov AE, Naumenko LV, Agarkov DIu, Sorokina EV. Hemorheological activity of Gymnema sylvestre extracts in streptozotocin-induced diabetes. Eksp Klin Farmakol 2008;71:31–3 (in Russian).Search in Google Scholar

[37] Xue WL, Li XS, Zhang J, Liu YH, Wang ZL, Zhang RJ. Effect of Trigonella foenum-graecum (fenugreek) extract on blood glucose, blood lipid and hemorheological properties in streptozotocin-induced diabetic rats. Asia Pac J Clin Nutr 2007;16:422–6.Search in Google Scholar

[38] Zibareva LN, Aliev OI, Koltunov AA, Plotnikov MB. Patent RU No 2160502 “Hemorheological medication”. Available at: (in Russian).Search in Google Scholar

[39] McMillan DE, Gion KM. Glucosylated hemoglobin and reduced erythrocyte deformability in diabetes. Horm Metab Res 1981;11:108–12.Search in Google Scholar

[40] Schwartz RS, Madsen JW, Rybicki AC, Nagel RL. Oxidation of spectrin and deformability defects in diabetic erythrocytes. Diabetes 1991;40:701–8.Search in Google Scholar

[41] Veiraiah A. Hyperglycemia, lipoprotein glycation, and vascular disease. Angiology 2005;56:431–8.Search in Google Scholar

[42] Watanabe H, Kobayashi A, Yamamoto T, Suzuki S, Hayashi H, Yamazaki N. Alterations of human erythrocyte membrane fluidity by oxygen-derived free radicals and calcium. Free Rad Biol Med 1990;8:507–14.Search in Google Scholar

[43] Huang Y, Liu D, Sun S. Mechanism of free radicals on the molecular fluidity and chemical structure of the red cell membrane damage. Clin Hemorheol Microcirc 2000;23:287–90.Search in Google Scholar

[44] Gayathri M, Kannabiran K. Effect of 2-hydroxy-4-methoxy benzoic acid isolated from Hemidesmus indicus on erythrocyte membrane bound enzymes and antioxidant status in streptozotocin-induced diabetic rats. Indian J Pharm Sci 2012;74:474–8.Search in Google Scholar

[45] Chandra A, Mahdi AA, Singh RK, Mahdi F, Chander R. Effect of Indian herbal hypoglycemic agents on antioxidant capacity and trace elements content in diabetic rats. J Med Food 2008;11:506–12.Search in Google Scholar

[46] Mohamed J, Shing SW, Idris MH, Budin SB, Zainalabidin S. The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes. Clinics (Sao Paulo) 2013;68:1358–63.Search in Google Scholar

[47] Kuzmenko AI, Morozova RP, Nikolenko IA, Korniets GV, Kholodova YuD. Effects of vitamin D3 and ecdysterone on free-radical lipid peroxidation. Biochemistry (Mosc) 1997;62:609–12.Search in Google Scholar

[48] Syrov VN. Phytoecdysteroids: their biological effects in the body of higher animals and the outlook for their use in medicine. Eksp Klin Farmakol 1994;57:61–6 (in Russian).Search in Google Scholar

[49] Symeonidis A, Athanassiou G, Psiroyannis A, Kyriazopoulou V, Kapatais-Zoumbos K, Missirlis Y, et al. Impairment of erythrocyte viscoelasticity is correlated with levels of glycosylated haemoglobin in diabetic patients. Clin Lab Haematol 2001;23:103–9.Search in Google Scholar

[50] Cinar Y, Senyol AM, Duman K. Blood viscosity and blood pressure: role of temperature and hyperglycemia. Am J Hypertens 2001;14:433–8.Search in Google Scholar

Received: 2017-02-28
Accepted: 2019-09-24
Published Online: 2019-11-09

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