Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter September 17, 2019

Antioxidant activity, carotenoids, chlorophylls and mineral composition from leaves of Pallenis spinosa: an Algerian medicinal plant

Hanane Amrani-Allalou , Lila Boulekbache-Makhlouf EMAIL logo , Paula Mapelli-Brahm , Sabrina Sait , Gian Carlo Tenore , Akila Benmeziane , Nabil Kadri , Khodir Madani and Antonio Jesús Meléndez Martínez

Abstract

Background

Plant and medicinal herbs are important sources of bioactive compounds and minerals that can play a role in preventing various diseases and they are considered a factor indispensable for the proper functioning of the human body.

Methods

We investigated the content of carotenoids and chlorophylls of leaves from Pallenis spinosa (P. spinosa), as well as their antioxidant activity and mineral composition then, we optimized the solvent extraction for the recovery of total carotenoids and chlorophylls using spectrophotometric method. Finally, we tested the antioxidant activity of the optimized extract by three assays (DPPH, ABTS and FRAP) and we determined the mineral composition by Emission Spectrometer Induced Couple Plasma (ICP).

Results

Carotenoid (CART), chlorophylls (CHLa + b), chlorophyll a (CHLA), chlorophyll b (CHLB) contents were about 36.337 ± 0.312; 347.769 ± 6.326; 224.286 ± 5.601; 123.483 ± 1.339 mg/100 g dw, respectively. We revealed an interesting antioxidant capacity by the tested extract (DPPH: 127.522 ± 1.406 mmol ET/Kgdw, ABTS: 104.827 ± 1.222 mmol ET/Kgdw and FRAP 71.89 ± 0.495 ± 0.994 mmol ET/Kgdw). Carotenoids and chlorophylls content correlate positively with the antioxidant activity of P. spinosa leaves extract (r=0.646–0.986). Eight minerals have been detected (Mg, Ca, P, Fe, Mn, Zn, Cu and Cr), Mg and Ca being the predominant ones (6479.32 ± 48.33 and 3851.88 ± 130.63 mg/Kg, respectively).

Conclusions

These results have shown that P. spinosa leaves are a good source of carotenoids and chlorophylls with a potent antioxidant potential with high amount of minerals.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

[1] Barroso MF, Ramalhosa MJ, Alves RC, Dias A, Soares CM, Oliva-Teles MT, et al. Total antioxidant capacity of plant infusions: assessment using electrochemical DNA-based biosensor and spectrophotometric methods. Food Control 2016;68:153–61.10.1016/j.foodcont.2016.03.029Search in Google Scholar

[2] Cazzonelli CI, Roberts AC, Carmody ME, Pogson BJ. Transcriptional control of set domain group 8 and carotenoid isomerase during arabidopsis development. Mol Plant 2010;3:174–91.10.1093/mp/ssp092Search in Google Scholar

[3] Stinco CM, Benítez-González AM, Hernanz D, Vicario IM, Meléndez-Martínez AJ. Development and validation of a rapid resolution liquid chromatography method for the screening of dietary plant isoprenoids: carotenoids,tocopherols and chlorophylls. J Chromatogr A 2014;1370:162–70.10.1016/j.chroma.2014.10.044Search in Google Scholar

[4] Stahl W, Sies H. Antioxidant activity of carotenoids. Mol Aspects Med 2003;24:345–51.10.1016/S0098-2997(03)00030-XSearch in Google Scholar

[5] Wang L, Liu Y. Optimization of solvent extraction conditions for total carotenoids in rapeseed using response surface methodology. Natural Science 2009;1:23–9.10.4236/ns.2009.11005Search in Google Scholar

[6] Aparicio-Ruiz R, Gandul-Rojas B. Decoloration kinetics of chlorophylls and carotenoids in virgin olive oil by autoxidation. Food Res Int 2014;65,Part B:199–206.10.1016/j.foodres.2014.05.046Search in Google Scholar

[7] Bakó E, Deli J, Tóth G. HPLC study on the carotenoid composition of Calendula products. J Biochem Biophys Methods 2002;53:241–50.10.1016/S0165-022X(02)00112-4Search in Google Scholar

[8] Ingkasupart P, Manochai B, Song WT, Hong JH. Antioxidant activities and lutein content of 11 marigold cultivars (Tagetes spp.) grown in Thailand. Food Sci Technol 2015;35:380–5.10.1590/1678-457X.6663Search in Google Scholar

[9] Straumite E, Kruma Z, Galoburda R. Pigments in mint leaves and stems. Agron Res 2015;13:1104–11.Search in Google Scholar

[10] Amin Mir M, Kumar N, Dogra P, Ahmad B. Analysis on the food value and chlorophyll contents of various plant species of Dehradun. AJRBPS 2014;2:150–8.Search in Google Scholar

[11] Pohndorf RS, Jr TRS C, Pinto LA. Kinetics and thermodynamics adsorption of carotenoids and chlorophylls in rice bran oil bleaching. J Food Eng 2016;185:9–16.10.1016/j.jfoodeng.2016.03.028Search in Google Scholar

[12] Chen PX, Tang Y, Marcone MF, Pauls PK, Zhang B, Liu R, et al. Characterization of free, conjugated and bound phenolics and lipophilic antioxidants in regular- and non-darkening cranberry beans (Phaseolus vulgaris L.). Food Chem 2015;185:298–308.10.1016/j.foodchem.2015.03.100Search in Google Scholar PubMed

[13] Pathan A, Kamble A, Chowdhury S, Laware S. Sequential extraction and quantification of Tinospora cordifolia leaf pigments and metabolites. Int J Curr Microbiol App Sci 2015;4:638–44.Search in Google Scholar

[14] Telgenhoff D, Lam K, Ramsay S, Vasquez V, Villareal K, Slusarewicz P, et al. Influence of papain urea copper chlorophyllin on wound matrix remodeling. Wound Rep Reg 2007;15:727–35.10.1111/j.1524-475X.2007.00279.xSearch in Google Scholar

[15] Tachino N, Guo D, Dashwood WM, Yamane S, Larsen R, Dashwood R. Mechanisms of the in vitro antimutagenic action of chlorophyllin against benzo[a]pyrene: studiesof enzyme inhibition, molecular complex formation and degradation of the ultimate carcinogen. Mutat Res 1994;308:191–203.10.1016/0027-5107(94)90154-6Search in Google Scholar

[16] Dashwood R, Yamane S, Larsen R. Study of the forces stabilizing complexes between chlorophylls and heterocyclic amine mutagens. Environ Mol Mutagen 1996;27:211–8.10.1002/(SICI)1098-2280(1996)27:3<211::AID-EM6>3.0.CO;2-HSearch in Google Scholar

[17] Imelouane B, Tahri M, Elbastrioui M, Aouinti F, Elbachiri A. Mineral contents of some medicinal and aromatic plants growing in eastern morocco. J Mater Environ Sci 2011;2:104–11.Search in Google Scholar

[18] Ansari JA, Ahmad MK, Verma AK, Fatima N, Khan HJ, Waseem M, et al. Microwave assisted determination of minerals and toxic metals in traditionally used medicinal plant Zingiber officinale roscoe by inductively coupled plasma-optical emission spectrometer. Int J Adv Res 2015;3:879–87.Search in Google Scholar

[19] Subramanian R, Subbramaniyan P, Raj V. Determination of some minerals and trace elements in two tropical medicinal plants. Asian Pac J Trop Biomed 2012;2:S555–S558.10.1016/S2221-1691(12)60273-1Search in Google Scholar

[20] Wu Q-X, Shi Y-P, Jia Z-J. Eudesmane sesquiterpenoids from the Asteraceae family. Nat Prod Rep 2006;23:699–734.10.1039/b606168kSearch in Google Scholar

[21] Hegazy A, Boulos L, Kabiel H, Sharashy O. Vegetation and species altitudinal distribution in Al-Jabal Al-Akhdar landscape, Libya. Pak J Bot 2011;43:1885–98.Search in Google Scholar

[22] Lichtenthaler HK, Buschmann C. Extraction of phtosynthetic tissues: chlorophylls and carotenoids. Curr Protoc Food Anal Chem 2001;1:F4.2.1–F4.2.6.10.1002/0471142913.faf0402s01Search in Google Scholar

[23] Lichtenthaler HK. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Lester Packer, Roland Douce, editors. Methods in enzymology, vol. 148. Academic Press, 1987:350–82.Search in Google Scholar

[24] Jiménez-Escrig A, Jiménez-Jiménez I, Sánchez-Moreno C, Saura-Calixto F. Evaluation of free radical scavenging of dietary carotenoids by the stable radical 2, 2-diphenyl-1-picrylhydrazyl. J Sci Food Agric 2000;80:1686–90.10.1002/1097-0010(20000901)80:11<1686::AID-JSFA694>3.0.CO;2-YSearch in Google Scholar

[25] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26:1231–7.10.1016/S0891-5849(98)00315-3Search in Google Scholar

[26] Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Comp Anal 2006;19:669–75.10.1016/j.jfca.2006.01.003Search in Google Scholar

[27] Warkoyo SE. The solvent effectiveness of extraction process on seaweed pigment. Makara Teknologi 2011;15:5–8.Search in Google Scholar

[28] Nobre B, Marcelo F, Passos R, Beirão L, Palavra A, Gouveia L, et al. Supercritical carbon dioxide extraction of astaxanthin and other carotenoids from the microalga Haematococcus pluvialis. Eur Food Res Technol 2006;223:787–90.10.1007/s00217-006-0270-8Search in Google Scholar

[29] Denery JR, Dragull K, Tang C, Li QX. Pressurized fluid extraction of carotenoids from Haematococcus pluvialis and Dunaliella salina and kavalactones from Piper methysticum. Anal Chim Acta 2004;501:175–81.10.1016/j.aca.2003.09.026Search in Google Scholar

[30] Suzuki Y, Shioi Y. Identification of chlorophylls and carotenoids in major teas by high-performance liquid chromatography with photodiode array detection. J Agric Food Chem 2003;51:5307–14.10.1021/jf030158dSearch in Google Scholar PubMed

[31] Khettaf A, Belloula N, Dridi S. Antioxidant activity, phenolic and flavonoid contents of some wild medicinal plants in southeastern Algeria. Afr J Biotechnol 2016;15:524–30.10.5897/AJB2015.14459Search in Google Scholar

[32] Pasquet V, J-R C, Farhat F, Thiéry V, Piot J-M, J-B B, et al. Study on the microalgal pigments extraction process: performanceof microwave assisted extraction. Process Biochem 2011;46:59–67.10.1016/j.procbio.2010.07.009Search in Google Scholar

[33] Johan F, Jafri M, Lim H, Maznah WW Laboratory measurement: chlorophyll-aconcentration measurement with acetone method using spectrophotometer, 2015:744–8.10.1109/IEEM.2014.7058737Search in Google Scholar

[34] Jinasena M, Amarasinghe A, Amarasinghe B, Prashantha M. Extraction and degradation of chlorophyll a and b from alternanthera sessilis. J Natl Sci Found 2016;44:11–21.10.4038/jnsfsr.v44i1.7977Search in Google Scholar

[35] Stramarkou M, Papadaki S, Kyriakopoulou K, Krokida M. Recovery of functional pigments from four different species of microalgae. IOSR-JESTFT 2016;10:26–30.10.9790/2402-1009022630Search in Google Scholar

[36] Mitić V, Stankov-Jovanović V, Dimitrijević M, Cvetković J, Petrović G, Stojanović G. Chemometric analysis of chlorophyll a, b and carotenoid content in green leafy vegetables. Biologica Nyssana 2013;4:45–55.Search in Google Scholar

[37] Zakynthinos G, Varzakas T. Carotenoids: from plants to food industry. Current Res Nutr Food Sci 2016;4:38–51.10.12944/CRNFSJ.4.Special-Issue1.04Search in Google Scholar

[38] Žnidarčič D, Ban D, Šircelj H. Carotenoid and chlorophyll composition of commonly consumed leafy vegetables in Mediterranean countries. Food Chem 2011;129:1164–8.10.1016/j.foodchem.2011.05.097Search in Google Scholar PubMed

[39] Cervantes-Paz B, JdJ O-P, Ruiz-Cruz S, Rios-Velasco C, Ibarra-Junquera V, Yahia EM, et al. Effects of pectin on lipid digestion and possible implications for carotenoid bioavailability during pre-absorptive stages: a review. Food Res Int 2017;2:1–49.10.1016/j.foodres.2017.02.012Search in Google Scholar PubMed

[40] Žilić S, Janković M, Basić Z, Vančetović J, Maksimović V. Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L): comparisonwith medicinal herbs. J Cereal Sci 2016;69:363–70.10.1016/j.jcs.2016.05.003Search in Google Scholar

[41] Endo Y, Usuki R, Kaneda T. Antioxidant effects of chlorophyll and pheophytin on the autoxidation of oils in the dark. II. The mechanism of antioxidative action of chlorophyll. J Am Oil Chem Soc 1985;62:1387–90.10.1007/BF02545965Search in Google Scholar

[42] Medjahdi B, Ibn Tattou M, Barkat D, Benabedli K. La flore vasculaire des monts des Trara (nord ouest algérien). Acta Bot Malacit 2009;34:57–75.10.24310/abm.v34i0.6917Search in Google Scholar

[43] Batta A. A study design of clinical intervention of chromium in diabetics and its biological significance. European J Biomed Pharm Sci 2017;4:147–51.Search in Google Scholar

[44] Breinholt V, Schimerlik M, Dashwood R, Bailey G. Mechanisms of chlorophyllin anticarcinogenesis against aflatoxin B1: complexformation with the carcinogen. Chem Res Toxicol 1995;8:506–14.10.1021/tx00046a004Search in Google Scholar

[45] Hiraki A, Yukawa M, Kim J, Ueda S. Antiviral substance from silkworm faeces: purificationand its chemical characterization. Biol Pharm Bull 1997;20:547–55.10.1248/bpb.20.547Search in Google Scholar

[46] Bouslama L, Hayashi K, Lee J-B, Ghorbel A, Hayashi T. Potent virucidal effect of pheophorbide a and pyropheophorbide a on enveloped viruses. J Nat Med 2011;65:229–33.10.1007/s11418-010-0468-8Search in Google Scholar

[47] Ozenda P. Flora and vegetation of the Sahara. Paris: CNRS, 1991.Search in Google Scholar

[48] Quezel P, Santa S Nouvelle Flore de l’Algerie et des regions desertiques et meridionales, Tome II, edition CNRS, Paris, 1963. Google Scholar 1963:1016.Search in Google Scholar

[49] Bammi J, Douira A. Contribution à la connaissance de la flore vasculaire de la forêt de l’Achach, plateau central (Maroc). Acta Bot Malacit 2004;29:23–41.10.24310/abm.v29i0.7220Search in Google Scholar

[50] Zareh MM. Synopsis of the family Asteraceae in Egypt. Int J Agri Biol 2005;7:832–44.Search in Google Scholar

[51] Francisco-Ortega J, Park S-J, Santos-Guerra A, Benabid A, Jansen RK. Origin and evolution of the endemic Macaronesian Inuleae (Asteraceae): evidencefrom the internal transcribed spacers of nuclear ribosomal DNA. Biol J Linn Soc 2001;72:77–97.10.1111/j.1095-8312.2001.tb01302.xSearch in Google Scholar

[52] Benítez G, González-Tejero M, Molero-Mesa J. Pharmaceutical ethnobotany in the western part of Granada province (southern Spain): ethnopharmacologicalsynthesis. J Ethnopharmacol 2010;129:87–105.10.1016/j.jep.2010.02.016Search in Google Scholar

[53] Agelet A, Valles J. Studies on pharmaceutical ethnobotany in the region of Pallars (Pyrenees, Catalonia, Iberian Peninsula). Part II. New or very rare uses of previously known medicinal plants. J Ethnopharmacol 2003;84:211–27.10.1016/S0378-8741(02)00319-7Search in Google Scholar

[54] Boudjelal A, Henchiri C, Sari M, Sarri D, Hendel N, Benkhaled A, et al. Herbalists and wild medicinal plants in M’Sila (North Algeria): anethnopharmacology survey. J Ethnopharmacol 2013;148:395–402.10.1016/j.jep.2013.03.082Search in Google Scholar

[55] Bouabdelli F, Djelloul A, Kaid-Omar Z, Semmoud A, Addou A. Antimicrobial activity of 22 plants used in urolithiasis medicine in Western Algeria. Asian Pac J Trop Dis 2012;2:S530–S535.10.1016/S2222-1808(12)60215-1Search in Google Scholar

[56] MaJ P-V, Robledo A. Anti-insect activity of plant extracts from the wild flora in southeastern Spain. Biochem Syst Ecol 1999;27:1–10.10.1016/S0305-1978(98)00051-9Search in Google Scholar

[57] Ahmed AA, Jakupovic J, Bohlmann F. Dihydroxypallenone, a sesquiterpene with a new carbon skeleton from Pallenis spinosa. Phytochemistry 1990;29:3355–8.10.1016/0031-9422(90)80215-3Search in Google Scholar

[58] Appendino G, Cravotto G, Gariboldi P, Claudi F, Picci V. A sesquiterpene alcohol from Pallenis spinosa. Phytochemistry 1989;28:849–50.10.1016/0031-9422(89)80127-XSearch in Google Scholar

[59] Appendino G, Jakupovic J, Jakupovic S. Sesquiterpenoids from Pallenis spinosa. Phytochemistry 1997;46:1039–43.10.1016/S0031-9422(97)00386-5Search in Google Scholar

[60] Sanz JF, Marco JA. A germacrane derivative from Pallenis spinosa. Phytochemistry 1991;30:2788–90.10.1016/0031-9422(91)85146-QSearch in Google Scholar

[61] Ahmed AA, Spaller M, Mabry TJ. Flavonoids of Pallenis spinosa (Asteraceae). Biochem Syst Ecol 1992;20:785–6.10.1016/0305-1978(92)90038-FSearch in Google Scholar

[62] Senatore F, Bruno M. Composition of the essential oil of Pallenis spinosa (L.) Cass. (Asteraceae). Flavour Frag J 2003;18:195–7.10.1002/ffj.1180Search in Google Scholar

[63] Ravi Kumar S, Vallikannan B. Carotenoid composition and retinol equivalent in plants of nutritional and medicinal importance: efficacy of β-carotene from Chenopodium album in retinol-deficient rats. Food Chem 2010;119:1584–90.10.1016/j.foodchem.2009.09.047Search in Google Scholar

Received: 2017-06-05
Accepted: 2019-04-13
Published Online: 2019-09-17

© 2019 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 30.1.2023 from https://www.degruyter.com/document/doi/10.1515/jcim-2017-0081/html
Scroll Up Arrow