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Beneficial effect of ghee consumption over mustard oil on lipid profile: A study in North Indian adult population

Hanjabam Barun Sharma, Soniya Vyas, Jayant Kumar and Soumen Manna



Ghee (G) is attributed with numerous health benefits in Ayurveda. However, due to the high saturated fat content, it has been predicted to increase the cardiovascular disease risk. Hence, the current study was performed to evaluate the effect of G consumption as compared to mustard oil (MO) on lipid profile.


Two hundred (100 males) apparently healthy adults (≥40 years) were randomly selected out of the total individuals interviewed in a house-to-house survey. They were divided into three groups based on G and MO consumption: (A) MO >1 L/month, G<0.5 kg/month; (B) MO 1–0.5 L/month, G 1.25–0.5 kg/month; and (C) MO <0.5–0.2 L/month, G>1.25 kg/month. Serum lipid parameters were compared among the groups.


Group C had the significantly lowest triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), TC/HDL and LDL/HDL and highest high-density lipoprotein (HDL). A similar finding was found when analysis was done separetely for male and female.


A favorable lipid profile might suggest a possible beneficial effect of predominantly G consumption over MO.


The authors would like to express their sincere acknowledgement to all participants as well as their family members. They further would like to thank all the teachers in Dr S. N. Medical College, Jodhpur, India.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

  6. Conflicts of Interest of each author/contributor: There are no conflicts of interest.

  7. A statement that the manuscript has been read and approved by all the authors: All authors have checked and approved the manuscript. The requirements for authorship as stated earlier in this document have been met. Author believes that the manuscript represents honest work.


[1] Sserunjogi ML, Abrahamsen RK, Narvhus J. A review paper: current knowledge of ghee and related products. Int Dairy J. 1998;8(8):677–88.10.1016/S0958-6946(98)00106-X Search in Google Scholar

[2] Kumar MV, Sambaiah K, Lokesh BR. Hypocholesterolemic effect of anhydrous milk fat ghee is mediated by increasing the secretion of biliary lipids. J Nutr Biochem. 2000;11(2):69–75.1071559010.1016/S0955-2863(99)00072-8 Search in Google Scholar

[3] Joshi KS. Docosahexaenoic acid content is significantly higher in ghrita prepared by traditional Ayurvedic method. J Ayurveda Integr Med. 2014;5(2):85–88.10.4103/0975-9476.13173024948858 Search in Google Scholar

[4] Ghafoorunissa. Dietary lipids and heart disease–the Indian context. National Med J India. 1994;7(6):270–76. Search in Google Scholar

[5] Shankar SR, Yadav RK, Ray RB, Bijlani RL, Baveja T, Jauhar N, et al. Serum lipid response to introducing ghee as a partial replacement for mustard oil in the diet of healthy young Indians. Indian J Physiol Pharmacol. 2005;49(1):49–56.15881858 Search in Google Scholar

[6] Mishra S, Manchanda S. Cooking oils for heart health. J Prev Cardiol. 2012;1:123–31. Search in Google Scholar

[7] Sharma H, Zhang X, Dwivedi C. The effect of ghee (clarified butter) on serum lipid levels and microsomal lipid peroxidation. Ayu. 2010;31(2):134–40.10.4103/0974-8520.7236122131700 Search in Google Scholar

[8] Jacobson MS. Cholesterol oxides in Indian ghee: possible cause of unexplained high risk of atherosclerosis in Indian immigrant populations. Lancet. 1987;2(8560):656–58.2887943 Search in Google Scholar

[9] Raheja BS. Ghee, cholesterol, and heart disease. Lancet. 1987;2(8568):1144.2890036 Search in Google Scholar

[10] Vagbhatta A. In: Vaidya Harishastri Paradkar, editor(s). Ashtang Hridaya, Sutra Sthana, Reprinted ed. New Delhi: Rashtriya Sanskrit Samsthana, 2002:73–74. Search in Google Scholar

[11] Siri-Tarino PW, Sun Q, Hu FB, Krauss RM. Meta-analysis of prospective cohort studies evaluating the association of saturated fat with cardiovascular disease. Am J Clinical Nutr. 2010;91(3):535–46.10.3945/ajcn.2009.27725 Search in Google Scholar

[12] De Souza RJ, Mente A, Maroleanu A, Cozma AI, Ha V, Kishibe T, et al. Intake of saturated and trans unsaturated fatty acids and risk of all cause mortality, cardiovascular disease, and type 2 diabetes: systematic review and meta-analysis of observational studies. BMJ. 2015;351:h3978.26268692 Search in Google Scholar

[13] Nelson RH. Hyperlipidemia as a risk factor for cardiovascular disease. Primary Care. 2013;40(1):195–211.10.1016/j.pop.2012.11.00323402469 Search in Google Scholar

[14] Ghafoorunissa. Antiatherogenic potential of oils in Indian Subjects consuming cereal based diets. Proc Nutr Soc India. 1999;46:33–46. Search in Google Scholar

[15] Shankar SR, Bijlani RL, Baveja T, Jauhar N, Vashisht S, Mahapatra SC, et al. Effect of partial replacement of visible fat by ghee (clarified butter) on serum lipid profile. Indian J Physiol Pharmacol. 2002;46(3):355–60.12613401 Search in Google Scholar

[16] Singhal S, Goyle A, Gupta R. Quantitative food frequency questionnaire and assessment of dietary intake. National Med J India. 1998;11(6):268–75. Search in Google Scholar

[17] Gupta R, Prakash H. Association of dietary ghee intake with coronary heart disease and risk factor prevalence in rural males. J Indian Med Assoc. 1997;95(3):67–9, 83. Search in Google Scholar

[18] Tremblay AJ, Morrissette H, J-M G, Bergeron J, Gagné C, Couture P. Validation of the Friedewald formula for the determination of low-density lipoprotein cholesterol compared with β-quantification in a large population. Clin Biochem. 2004;37(9):785–90.10.1016/j.clinbiochem.2004.03.008 Search in Google Scholar

[19] Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18(6):499–502.4337382 Search in Google Scholar

[20] Sharma HB, Gender KJ. Difference in aerobic capacity and the contribution by body composition and haemoglobin concentration: a study in young Indian national hockey players. J Clin Diagn Res. 2016;10(11):CC09–CC13. Search in Google Scholar

[21] Hanjabam B, Meitei KK. Anthropometric basis for the physiological demand of anaerobic power and agility in young Indian national level field hockey players. Fiziologia - Physiology. 2015;25.3(87):41–48. Search in Google Scholar

[22] Field A. Discovering statistics using SPSS, 3rd ed. India: SAGE, 2009. Search in Google Scholar

[23] Raheja BS. Dietary fats and habits and susceptibility of Asian Indians to NIDDM and atherosclerotic heart disease. J Diabet Assoc India. 1991;31:21–28. Search in Google Scholar

[24] Ramsden CE, Zamora D, Leelarthaepin B, Majchrzak-Hong SF, Faurot KR, Suchindran CM, et al. Use of dietary linoleic acid for secondary prevention of coronary heart disease and death: evaluation of recovered data from the Sydney Diet Heart Study and updated meta-analysis. BMJ. 2013;346:e8707.10.1136/bmj.e870723386268 Search in Google Scholar

[25] Wendlinger C, Hammann S, Vetter W. Various concentrations of erucic acid in mustard oil and mustard. Food Chem. 2014;153:393–97.10.1016/j.foodchem.2013.12.07324491745 Search in Google Scholar

[26] Dixit S, Das M. Fatty acid composition including trans-fatty acids in edible oils and fats: probable intake in Indian population. J Food Sci. 2012;77(10):T188–99.2295053810.1111/j.1750-3841.2012.02875.x Search in Google Scholar

[27] Kumar MV, Sambaiah K, Mangalgi SG, Murthy NA, Lokesh BR. Effect of medicated ghee on serum lipid levels in psoriasis patients. Indian J Dairy & Biosci. 1999;10:20–23. Search in Google Scholar

[28] Nath BS, Usha MA, Mk R. Effect of deep frying on cholesterol oxidation in ghee. J Food Sci Technol. 1996;33:425–26. Search in Google Scholar

[29] Bhatia E, Kansal VK. Dairy Ghee opposed to soybean oil attenuates diet induced hypercholesterolemia in rats. Milchwissenschaft. 2011;66(1):15–18. Search in Google Scholar

[30] Baudet MF, Jacotot B. Dietary fats and lecithin-cholesterol acyltransferase activity in healthy humans. Ann Nutr Metab. 1988;32(5-6):352–59.325469110.1159/000177491 Search in Google Scholar

[31] Osada K, Sasaki E, Sugano M. Lymphatic absorption of oxidized cholesterol in rats. Lipids. 1994;29(8):555–59.10.1007/BF025366277990662 Search in Google Scholar

[32] Goldstein JL, Brown MS. Regulation of the mevalonate pathway. Nature. 1990;343(6257):425–30.196782010.1038/343425a0 Search in Google Scholar

[33] Jiang J, Wolk A, Vessby B. Relation between the intake of milk fat and the occurrence of conjugated linoleic acid in human adipose tissue. Am J Clin Nutr. 1999;70(1):21–27.1039313410.1093/ajcn/70.1.21 Search in Google Scholar

[34] Lee KN, Kritchevsky D, Pariza MW. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis. 1994;108(1):19–25.10.1016/0021-9150(94)90034-57980704 Search in Google Scholar

[35] Nicolosi RJ, Rogers EJ, Kritchevsky D, Scimeca JA, Huth PJ. Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery. 1997;22(5):266–77.9209699 Search in Google Scholar

[36] Mougios V, Matsakas A, Petridou A, Ring S, Sagredos A, Melissopoulou A, et al. Effect of supplementation with conjugated linoleic acid on human serum lipids and body fat. J Nutr Biochem. 2001;12(10):585–94.1203126410.1016/S0955-2863(01)00177-2 Search in Google Scholar

[37] Yu S, Derr J, Etherton TD, Kris-Etherton PM. Plasma cholesterol-predictive equations demonstrate that stearic acid is neutral and monounsaturated fatty acids are hypocholesterolemic. Am J Clin Nutr. 1995;61(5):1129–39.773303910.1093/ajcn/61.5.1129 Search in Google Scholar

[38] German JB, Dillard CJ. Saturated fats: what dietary intake?. Am J Clin Nutr. 2004;80(3):550–59.10.1093/ajcn/80.3.55015321792 Search in Google Scholar

[39] Molkentin J. Occurrence and biochemical characteristics of natural bioactive substances in bovine milk lipids. British J Nutr. 2000;84(Suppl 1):S47–53. Search in Google Scholar

[40] Spiteller G. The important role of lipid peroxidation processes in aging and age dependent diseases. Mol Biotechnol. 2007;37(1):5–12.1791415710.1007/s12033-007-0057-6 Search in Google Scholar

[41] Raheja BS. Diabetes and atherosclerosis as immune-inflammatory disorders: options for reversal of disease processes. J Assoc Phys India. 1994;42(5):385–90, 95-6. Search in Google Scholar

[42] Strobel C, Jahreis G, Kuhnt K. Survey of n-3 and n-6 polyunsaturated fatty acids in fish and fish products. Lipids Health Disease. 2012;11:144.10.1186/1476-511X-11-144 Search in Google Scholar

[43] Dhiman TR, Anand GR, Satter LD, Pariza MW. Conjugated linoleic acid content of milk from cows fed different diets. J Dairy Sci. 1999;82(10):2146–56.1053160010.3168/jds.S0022-0302(99)75458-5 Search in Google Scholar

[44] Tyagi A, Kewalramani N, Dhiman T, Kaur H, Singhal K, Kanwajia S. Enhancement of the conjugated linoleic acid content of buffalo milk and milk products through green fodder feeding. Anim Feed Sci Technol. 2007;133(3):351–58.10.1016/j.anifeedsci.2006.05.003 Search in Google Scholar

Received: 2017-08-04
Accepted: 2017-12-21
Published Online: 2018-01-24

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