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Licensed Unlicensed Requires Authentication Published by De Gruyter March 23, 2018

Evaluating of β-carotene role in ameliorating of favism-induced disturbances in blood and testis

Khaled M. M. Koriem and Mahmoud S. Arbid

Abstract

Background

Favism is an acute hemolytic anemia occurs in glucose 6-phosphate dehydrogenase (G6-PD) deficient individuals. β-Carotene occurs in vegetables such as carrots. This study aimed to establish the therapeutic effect of β-carotene to rebalance the testicular and blood proteins disturbances in favism.

Methods

Forty-eight male rats were divided into six equal groups; Groups 1, 2 and 3: normal rats were daily oral administrated with 1 ml saline, 1 ml corn oil and β-carotene (60 mg/kg dissolved in 1 ml corn oil), respectively, once a day over 15 days period. Group 4 (favism-induced group): normal rats injected intraperitoneal (ip) with diethyl maleate (5 μl/rat) and after 1 h injected ip with 1/3 LD50 of faba beans ethanolic extract for 15 day to induce favism. Groups 5 and 6: favism-induced rats were daily oral administered with 30 and 60 mg/kg β-carotene dissolved in 1 ml corn oil, respectively, once a day over 15 days.

Results

The results revealed that oral administration of corn oil or β-carotene into normal rats over 15 days period did not induce any change. In favism-induced groups, hematological parameters, liver function, serum glucose, G6-PD, luteinizing and follicle-stimulating hormones and sex-hormone binding globulin showed significant increase. Moreover, serum testosterone and dehydroepiandrosterone sulfate, testicular G6-PD, 3β-hydroxy steroid dehydrogenase, cholesterol and total protein were decreased. Treatment with both doses of β-carotene into favism groups restored all the abovementioned parameters to approach normal values. Favism inhibited blood proteins while β-carotene treatment into favism group stopped blood cells damage and blood proteins inhibition. These results were supported by histological studies.

Conclusions

In conclusion, taken β-carotene into favism group abolished testicular and blood proteins disturbances and this effect was dose dependent.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

[1] McMillan DC, Jollow DJ. Favism: divicine hemotoxicity in the rat. Toxicol Sci. 1999;51:310–16.10.1093/toxsci/51.2.310Search in Google Scholar PubMed

[2] Bicakci Z. A hemolysis trigger in glucose-6-phosphate dehydrogenase enzyme deficiency. Vicia sativa (Vetch). Saudi Med J. 2009;30:292–94.Search in Google Scholar PubMed

[3] Hegazy MI, Marquardt RR. Metabolism of vicine and convicine in rat tissues: absorption and excretion patterns and site of hydrolysis. J Sci Food Agric. 1984;35:139–46.10.1002/jsfa.2740350204Search in Google Scholar

[4] Arbid MS, Marquardt RR. Effect of intraperitoneally injected vicine and convicine on the rats: induction of favism-like sign. J Sci Food Agri. 1986;37:539–47.10.1002/jsfa.2740370606Search in Google Scholar

[5] Albano E, Tomasi A, Mannuzzu L, Arese P. Detection of free radical intermediate from divicine of Vicia faba. Biochem Pharmacol. 1984;33:1701–04.10.1016/0006-2952(84)90299-5Search in Google Scholar PubMed

[6] Marquardt RR. Vicine, convicine and their role aglycones-divine and isouramil. In: Cheeke P, editor. Toxicants of plant origin, 2nd ed. Boca Raton: CRC Press, 1989:614–23.Search in Google Scholar

[7] Gutierrez N, Avila CM, Duc G, Marget P, Suso MJ, Moreno MT, et al. CAPs markers to assist selection for low vicine and convicine contents in faba bean (Vicia faba L.). Theor Appl Genet. 2006;114:59–66.10.1007/s00122-006-0410-3Search in Google Scholar PubMed

[8] Arbid MS, Koriem KM, Asaad GF, Megahed HA. Effect of the antibiotic neomycin on the toxicity of the glycoside vicine in rats. J Toxicol. 2013;2013:8. Art ID 913128.10.1155/2013/913128Search in Google Scholar PubMed PubMed Central

[9] Koriem KM, Arbid MS, El-Gendy NF. The protective role of anise oil in oxidative stress and genotoxicity produced in favism. J Diet Suppl. 2016;13:505–21.10.3109/19390211.2015.1119775Search in Google Scholar PubMed

[10] Koriem KM, Arbid MS, Gomaa NE. Supplementation of α-tocopherol attenuates minerals disturbance, oxidative stress and apoptosis occurring in favism. Ind J Clin Biochem. 2017;32:446–52.10.1007/s12291-016-0623-4Search in Google Scholar PubMed PubMed Central

[11] Akçakaya H, Tok S, Dal F, Cinar SA, Nurten R. β-Carotene treatment alters the cellular death process in oxidative stress-induced K562 cells. Cell Biol Int. 2017;41:309–19.10.1002/cbin.10727Search in Google Scholar PubMed

[12] Wu L, Guo X, Hartson SD, Davis MA, He H, Medeiros DM. Lack of β, β-carotene-9´, 10´-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice. Mol Nutr Food Res. 2017;61:1600576 .10.1002/mnfr.201600576Search in Google Scholar PubMed PubMed Central

[13] Hu Y, Cui J, Sparks JA, Malspeis S, Costenbader KH, Karlson EW, et al. Circulating carotenoids and subsequent risk of rheumatoid arthritis in women. Clin Exp Rheumatol. 2017;35:309–12Search in Google Scholar PubMed

[14] Koriem KM, Arbid MS, El-Gendy NF. The protective effect of some antioxidants against the toxic effect of the alcoholic extract of faba beans on albino rats. Rev Latinoamer Quím. 2009;37:181–93.Search in Google Scholar

[15] El-Shabrawy OA. Toxicological Studies on Vicia Faba in laboratory animals. M. Sc. Thesis Medical Jurisprudence. Egypt: Alexandria University, 1971:86–91.Search in Google Scholar

[16] Behren W, Karber G. Determination of LD50. Archiv Experim Pathol Pharmacol. 1953;2:177–277.Search in Google Scholar

[17] Jahn NH. Nutritional toxicology. New York, USA: Academic Press, 1982:28–31.Search in Google Scholar

[18] Uthus EO. The effect on arsenic deprivation in rats of diethyl maleate, an in vivo chemical depletory of glutathione. Proc New Drugs Acad Sci Technol. 1992;46:70–75.Search in Google Scholar

[19] Naziroğlu M, Cay M, Ustündağ B, Aksakal M, Yekeler H. Protective effects of vitamin E on carbon tetrachloride induced liver damage in rats. Cell Biochem Funct. 1999;17:253–59.10.1002/(SICI)1099-0844(199912)17:4<253::AID-CBF837>3.0.CO;2-RSearch in Google Scholar PubMed

[20] Lin C, Choi YS, Park SG, Gwon L, Lee JG, Yon JM, et al. Enhanced protective effects of combined treatment with β-carotene and curcumin against hyperthermic spermatogenic disorders in mice. BioMed Res Int. 2016;2016:2572073 .10.1155/2016/2572073Search in Google Scholar PubMed

[21] Dubois L, Andersson K, Asplund A, Björkelund H. Evaluating real-time immunohistochemistry on multiple tissue samples, multiple targets and multiple antibody labeling methods. BMC Res Notes. 2013;6:542.10.1186/1756-0500-6-542Search in Google Scholar PubMed

[22] Koriem KM, Megahed HA, Arbid MS. Evaluation of some adverse effects of the glycoside convicine in Sprague-Dawley rats. Toxicol Environ Chem. 2008;90:415–20.10.1080/02772240701483855Search in Google Scholar

[23] Comporti M. Lipid peroxidation and cellular damage in toxic liver injury. Lab Invest. 1985;53:599–623.Search in Google Scholar PubMed

[24] Aksak Karamese S, Toktay E, Unal D, Selli J, Karamese M, Malkoc I. The protective effects of beta-carotene against ischemia/reperfusion injury in rat ovarian tissue. Acta Histochem. 2015;117:790–97.10.1016/j.acthis.2015.07.006Search in Google Scholar PubMed

[25] El-Demerdash FM, Yousef MI, Kedwany FS, Baghdadi HH. Cadmium-induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and beta-carotene. Food Chem Toxicol. 2004;42:1563–71.10.1016/j.fct.2004.05.001Search in Google Scholar PubMed

[26] Vardi N, Parlakpinar H, Cetin A, Erdogan A, Cetin Ozturk I. Protective effect of beta-carotene on methotrexate-induced oxidative liver damage. Toxicol Pathol. 2010;38:592–97.10.1177/0192623310367806Search in Google Scholar PubMed

[27] Gumpricht E, Dahl R, Devereaux MW, Sokol RJ. Beta-carotene prevents bile acid-induced cytotoxicity in the rat hepatocyte: evidence for an antioxidant and anti-apoptotic role of beta-carotene in vitro. Pediatr Res. 2004;55:814–21.10.1203/01.PDR.0000117845.23762.6BSearch in Google Scholar PubMed

[28] Saradha B, Mathur PP. Effect of environmental contaminants on male reproduction. Environ Toxicol Pharmacol. 2006;21:34–41.10.1016/j.etap.2005.06.004Search in Google Scholar PubMed

[29] Xiao M, Du G, Zhong G, Yan D, Zeng H, Cai W. Gas chromatography/mass spectrometry-based metabolomic profiling reveals alterations in mouse plasma and liver in response to fava beans. PLoS One. 2016;11:e0151103.10.1371/journal.pone.0151103Search in Google Scholar

[30] Schmitz G, Hohage H, Ullrich K. Glucose-6-phosphate: a key compound in glycogenosis I and favism leading to hyper- or hypolipidaemia. Eur J Pediatr. 1993;152:S77–84.10.1007/BF02072094Search in Google Scholar PubMed

[31] Dessì S, Batetta B, Spano O, Pulisci D, Mulas MF, Muntoni S. Serum lipoprotein pattern as modified in G6PD-deficient children during haemolytic anaemia induced by fava bean ingestion. Int J Exp Pathol. 1992;73:157–60.Search in Google Scholar PubMed

[32] Osman HG, Zahran FM, El-Sokkary AM, El-Said A, Sabry AM. Identification of Mediterranean mutation in Egyptian favism patients. Eur Rev Med Pharmacol Sci. 2014;18:2821–27.Search in Google Scholar PubMed

[33] Arese P, Gallo V, Pantaleo A, Turrini F. Life and death of Glucose-6-Phosphate Dehydrogenase (G6PD) deficient erythrocytes – role of redox stress and band 3 modifications. Transfus Med Hemother. 2012;39:328–34.10.1159/000343123Search in Google Scholar PubMed PubMed Central

[34] Yang HC, Chen TL, Wu YH, Cheng KP, Lin YH, Cheng ML. Glucose 6-phosphate dehydrogenase deficiency enhances germ cell apoptosis and causes defective embryogenesis in Caenorhabditis elegans. Cell Death Dis. 2013;4:e616.10.1038/cddis.2013.132Search in Google Scholar PubMed PubMed Central

[35] Aguilar-Mahecha A, Hales BF, Robaire B. Chronic cyclophosphamide treatment alters the expression of stress response genes in rat male germ cells. Biol Reprod. 2002;66:1024–32.10.1095/biolreprod66.4.1024Search in Google Scholar PubMed

[36] Moore MJ. Clinical pharmacokinetics of cyclophosphamide. Clin Pharm. 1991;20:194–208.10.2165/00003088-199120030-00002Search in Google Scholar PubMed

Received: 2017-12-07
Accepted: 2018-02-20
Published Online: 2018-03-23

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